Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi

Betül Yılmaz Öztürk; Derviş Öztürk

doi:10.46309/biodicon.2020.764145

Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi

Abstract

Kimyasal reaksiyonlarla sentezlenen nanopartiküllerin çeşitli omurgalılarda ve omurgasızlarda geniş bir toksisite yelpazesi sergilese de antifungal aktivitelerinden yararlanmak üzere büyük miktarlarda üretilmektedir. Klasik kimyasal yöntemlerin dikkat çekici dezavantajları nedeniyle, metalik nanopartiküllerin yeşil sentezi son yıllarda büyük ilgi görmeye başlamıştır. Bu yöntem düşük maliyetli, çevre dostu ve basit yaklaşımları ile klasik kimyasal yöntemlere bir alternatif olarak önerilmektedir. Bu çalışmada amacımız, gümüş nanopartiküllerin hücre dışı sentezinde Tilia rubra DC. ekstraktının uygunluğunu belirlemek ve elde edilen materyali karakterize etmektir. Bunun yanısıra biyosentezlenmiş gümüş nanopartiküllerinin antifungal etkisi değerlendirilmiştir. T. rubra ekstraktı, gümüş nanopartiküllerin biyosentezi için ilk kez çalışılmış olup UV-Vis spektrofotometresinde 427 nm'de yüzey plazmon rezonansı oluşturdu. Ayrıca geçirimli elektron mikroskop (TEM) görüntüleri nanopartiküllerin küresel morfolojiye sahip olduğunu gösterdi. TEM görüntülerine göre nanopartiküllerin boyutlarının 5-15 nm arasında olduğu bulundu. X-ışını kırınımı (XRD) analizi ise partiküllerin yüz merkezli kübik geometriye sahip kristal yapıda olduğunu belirledi. Gümüş iyonlarının biyo-indirgenmesinden sorumlu olası biyomolekülleri tanımlamak için Fourier dönüşümlü kızılötesi spektroskopisi (FTIR) analizi yapıldı. Biyosentezlenmiş gümüş nanopartiküllerin patojen bir maya olan Candida albicans üzerindeki antifungal etkisi agar difüzyon metodu ile incelendi ve sonuç olarak sentezlenen gümüş nanopartiküllerinin, Candida albicans üzerinde önemli inhibe edici etki gösterdi. Biyosentezlenmiş gümüş nanopartiküllerin çevre dostu sentez prosedürü gelecekte endüstriyel ve biyomedikal uygulamalar için kullanılma potansiyeline sahip olduğunu desteklemektedir.

Keywords

Thanks

Bu çalışma Eskişehir Osmangazi Üniversitesi Merkez Araştırma Laboratuvarı Uygulama ve Araştırma Merkezi'nde (ARUM) çalışılmıştır.

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Details

Primary Language

Turkish

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Betül Yılmaz Öztürk ^*
0000-0002-1817-8240
Türkiye

Derviş Öztürk
0000-0001-7189-7407
Türkiye

Publication Date

December 15, 2020

Submission Date

July 4, 2020

Acceptance Date

October 26, 2020

Published in Issue

Year 2020 Volume: 13 Number: 3

DOI

https://doi.org/10.46309/biodicon.2020.764145

IZ

https://izlik.org/JA46SW74EE

Cite

RIS / Bibtex

APA

Yılmaz Öztürk, B., & Öztürk, D. (2020). Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi. Biological Diversity and Conservation, 13(3), 244-251. https://doi.org/10.46309/biodicon.2020.764145

AMA

1.Yılmaz Öztürk B, Öztürk D. Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi. BioDiCon. 2020;13(3):244-251. doi:10.46309/biodicon.2020.764145

Chicago

Yılmaz Öztürk, Betül, and Derviş Öztürk. 2020. “Tilia Rubra DC. Ekstraktı Kullanılarak Gümüş Nanopartikülün Hücre Dışı Biyosentezi Ve Antifungal Aktivitesi”. Biological Diversity and Conservation 13 (3): 244-51. https://doi.org/10.46309/biodicon.2020.764145.

EndNote

Yılmaz Öztürk B, Öztürk D (December 1, 2020) Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi. Biological Diversity and Conservation 13 3 244–251.

IEEE

[1]B. Yılmaz Öztürk and D. Öztürk, “Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi”, BioDiCon, vol. 13, no. 3, pp. 244–251, Dec. 2020, doi: 10.46309/biodicon.2020.764145.

ISNAD

Yılmaz Öztürk, Betül - Öztürk, Derviş. “Tilia Rubra DC. Ekstraktı Kullanılarak Gümüş Nanopartikülün Hücre Dışı Biyosentezi Ve Antifungal Aktivitesi”. Biological Diversity and Conservation 13/3 (December 1, 2020): 244-251. https://doi.org/10.46309/biodicon.2020.764145.

JAMA

1.Yılmaz Öztürk B, Öztürk D. Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi. BioDiCon. 2020;13:244–251.

MLA

Yılmaz Öztürk, Betül, and Derviş Öztürk. “Tilia Rubra DC. Ekstraktı Kullanılarak Gümüş Nanopartikülün Hücre Dışı Biyosentezi Ve Antifungal Aktivitesi”. Biological Diversity and Conservation, vol. 13, no. 3, Dec. 2020, pp. 244-51, doi:10.46309/biodicon.2020.764145.

Vancouver

1.Betül Yılmaz Öztürk, Derviş Öztürk. Tilia rubra DC. ekstraktı kullanılarak gümüş nanopartikülün hücre dışı biyosentezi ve antifungal aktivitesi. BioDiCon. 2020 Dec. 1;13(3):244-51. doi:10.46309/biodicon.2020.764145

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