Rhizopogon roseolus Corda Th. Fr.’ un kültürel özellikleri

Yıl 2017, Cilt: 10 Sayı: 3, 79 - 84, 15.04.2017

Perihan Güler

Öz

Bu çalışmada Rhizopogon roseolus Corda Th. Fr.’un spor ve miselyumlarının anatomik ve morfolojik özellikleri çalışıldı. Mantar örnekleri Kırıkkale-Türkiye’den toplandı ve laboratuvara getirildi. Alınan doku örnekleri patates dekstroz agar PDA besiyerinde doku kültürü yöntemi ile gelişti. 25˚C’de inkübe edildi. Miseller inokulasyondan 2 gün sonra besiyeri üzerinde karışık olarak gelişim gösterdi. Özellikle koloninin en dış bölgesindeki misellerde yoğunluğun arttığı ve pigmentasyon oluşumu gözlendi. Misellerde önce hafif sarımsı renkte gelişme olduğu daha sonra koloninin en dışında pembemsi pigmentasyon geliştiği görüldü. Kolonizasyon oluşumu 20 günde tamamlandı R.roseolus’un spor ve miselleri hem ışık hem de scanning elektron mikroskop SEM ile incelendi. R.roseolus’un sporları düz ve boyutları 3.5-4x6.5-7 µm. olup genellikle oval şekillidir

Anahtar Kelimeler

Rhizopogon roseolus, Türkiye mikobiyotası, Kırıkkale makromantarları, miselyum gelişimi

Cultural characteristics of Rhizopogon roseolus Corda Th. Fr

Öz

In this study, the morphological and anatomical features of Rhizopogon roseolus Corda Th. Fr. spores and mycelium were examined. The mushroom samples were collected from Kırıkkale-Turkey and brought to the laboratory. Taken tissue fragments were grown on potato dextrose agar PDA medium by tissue culture method. They were incubated in 25˚C. After 2 days from inoculation mycelium showed mixed growth on the agar medium. Particularly, in the outermost regions of the colony, density increased and pigmentation was observed. It was first observed to develop a slightly yellowish color and then pinkish pigmentation developed at the outermost part of the colony. Colonization occurred in 20 days. The spores and mycelium of R.roseolus were examined by both light microscopy and scanning electron microscopy SEM . The spores of R. roseolus are smooth and have dimensions of .5-4x6.5-7 µm. usually oval shape

Anahtar Kelimeler

Rhizopogon roseolus, Turkey mycobiota, Kırıkkale macrofungus, mycelium development

Kaynakça

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