Buğdaydan elde edilen Rhizoctonia anastomosis gruplarının moleküler karakterizasyonu

Yıl 2025, Cilt: 65 Sayı: 4, 100 - 110, 01.01.2026

Harun Bayraktar , Filiz Ünal , Fatma Sara Dolar

https://doi.org/10.16955/bitkorb.1551947

Öz

Orta Anadolu buğday tarlalarından toplanan bitki ve toprak örneklerinden izole edilen Rhizoctonia izolatları MseI, Ava II/HincII, MunI, MspI (HapII), HinfI, EcoRI, HaeIII, HinfI ve HhaI enzimleri kullanılarak PCR-RFLP ve DNA sekans analizine tabi tutulmuştur. Analiz sonucunda Rhizoctonia izolatlarının çok çekirdekli Rhizoctonia solani AG 2-1, AG 2 BI, AG 3, AG 4 HG II, AG 5, AG 8 ve Waitea circinata var. circinata, Waitea circinata var. zeae, Waitea circinata var. oryzae grup I, çift çekirdekli Rhizoctonia AG DI, AG E, AG G, AG I ve AG K olduğu belirlenmiştir. Filogenetik ağaçlarda aynı grup ve altgrupta yer alan izolatlar aynı grupta yer almışlardır. Farklı grup ve alt gruptaki izolatlar ise farklı dallarda yer alarak grup ve altgruplar arasındaki genetik farklılığı ortaya koymuştur.

Anahtar Kelimeler

Rhizoctonia , anastomosis grup , PCR , RFLP , buğday

Proje Numarası

TÜBİTAK 110O622

Molecular characterization of Rhizoctonia anastomosis groups from wheat

Öz

Rhizoctonia isolates isolated from plant and soil samples collected from Central Anatolian wheat fields were subjected to PCR-RFLP and DNA sequence analysis using MseI, Ava II/HincII, MunI, MspI (HapII), HinfI, EcoRI, HaeIII, HinfI and HhaI enzymes. As a result of the analysis, Rhizoctonia isolates were determined to be multinucleate Rhizoctonia solani AG 2-1, AG 2 BI, AG 3, AG 4 HG II, AG 5, AG 8, and Waitea circinata var. circinata, Waitea circinata var. zeae, Waitea circinata var. oryzae group I, binucleate Rhizoctonia AG DI, AG E, AG G, AG I and AG K. Isolates in the same group and subgroup in phylogenetic trees were in the same group. Isolates from different groups and subgroups were located in various branches, revealing the genetic differences between the groups and subgroups.

Anahtar Kelimeler

Rhizoctonia , anastomosis group , PCR , RFLP , wheat

Etik Beyan

Çalışmanın tüm süreçlerinin araştırma ve yayın etiğine uygun olduğunu beyan ederim.

Destekleyen Kurum

TÜBİTAK

Proje Numarası

TÜBİTAK 110O622

Teşekkür

This study was supported by The Scientific and Technological Research Council of Turkey

Kaynakça

• Boysen M., Borja M., del Moral C., Salazar O., Rubio V., 1996. Identification at strain level of Rhizoctonia solani AG 4 direct isolates by sequence of asymmetric PCR products of the ITS regions. Current Genetics, 29 (2), 174–181. doi: 10.1007/BF02221582
• Carling D.E., Kuninaga S., Brainard K.A., 2002. Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani anastomosis group-2 (AG-2) and AG-BI. Phytopathology, 92 (1), 43–50.
• Ceresini P.G., Shew H.D., Vilgalys R.J., Cubeta M.A., 2002. Genetic diversity of Rhizoctonia solani AG 3 from potato and tobacco in North Carolina. Mycologia, 94 (3), 437-449.
• Chen C., Cerda K.A., Kaminski J.E., Douhan G.W., Wong F.P., 2009. Geographic distribution and rDNA-ITS region sequence diversity of Waitea circinata var. circinata isolated from annual bluegrass in the United States. Plant Disease, 93 (9), 906-911. doi: 10.1094/PDIS-93-9-0906
• Cubeta M.A., Vilgalys R., 1997. Population Biology of the Rhizoctonia solani complex. Phytopathology, 87 (4), 480-484. https://doi.org/10.1094/PHYTO.1997.87.4.480
• Demirci E., 1998. Rhizoctonia species and anastomosis groups isolated from barley and wheat in Erzurum, Turkey. Plant Pathology, 47 (1), 10-15. https://doi.org/10.1046/j.1365-3059.1998.00214.x
• Dong W., Li Y., Duan C., Li X., Naito S., Conner R.L., Yang G., Li C., 2017. Identification of AG-V, a new anastomosis group of binucleate Rhizoctonia spp. from taro and ginger in Yunnan province. European Journal of Plant Pathology, 148 (4), 839-851.
• Erper I., Ozer G., Kalendar R., Avci S., Yildirim E., Alkan M., Turkkan M., 2021. Genetic diversity and pathogenicity of Rhizoctonia spp. isolates associated with red cabbage in Samsun (Turkey). Journal of Fungi, 7 (3), 234. doi: 10.3390/jof7030234
• González V., Salazar O., Julián M.C., Acero J., Portal M.A., Muñóz R., López-Córcoles H., Gómez-Acebo E., López-Fuster P., Rubio V., 2002. Ceratobasidium albasitensis. A new Rhizoctonia-like fungus isolated in Spain. Persoonia, Molecular Phylogeny and Evolution of Fungi, 17 (4), 601614.
• Guillemaut C., Edel-Hermann V., Camporota P., Alabouvette C., Richard-Molard M., Steinberg C., 2003. Typing of anastomosis groups of Rhizoctonia solani by restriction analysis of ribosomal DNA, Canadian Journal of Microbiology, 49 (9), 556–68. doi: 10.1139/w03-066
• Hyakumachi M., Priyatmojo A., Kubota M., Fukui H., 2005. New anastomosis groups, AG-T and AG-U, of binucleate Rhizoctonia causing root and stem rot of cut-flower and miniature roses. Phytopathology, 95 (7), 784–792. doi: 10.1094/PHYTO-95-0784
• Kumar S., Stecher G., Tamura K., 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evoluation, 33 (7), 1870-1874. doi: 10.1093/molbev/msw054
• Kuninaga S., 1996. DNA base sequence complementary analyses. In: Rhizoctonia species: taxonomy, molecular biology, ecology, pathology and disease control. Sneh B., Jabaji-Hare S.H., Neate S., Dijst G. (Eds.). Kluwer, Dordrecht, pp 73–80.
• Kuninaga S., Carling D.E., Takenuichi T., Yokosawa R., 2000. Comparison of rDNA-ITS sequences between potato and tobacco strains in Rhizoctonia solani AG-3. Journal of General Plant Pathology, 66 (1), 2-11. https://doi.org/10.1007/PL00012917
• Lee S.B., Taylor J.W., 1990. Isolation of DNA from fungal mycelia and single spores. In: PCR protocols: a guide to methods and applications. . Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., (Eds.). Academic Press, New York, 282-287 pp.
• Ma M., Tan T.K., Wong S.M., 2003. Identification and molecular phylogeny of Epulorhiza isolates from tropical orchids. Mycological Research, 107 (9), 1041–1049. doi: 10.1017/s0953756203008281
• Meyer L., Nel L.H., Carling D.E., 1997. Anastomosis grouping and molecular genetic typing of Rhizoctonia solani causing crater disease of wheat in South Africa. Diagnosis and identification of plant pathogens. Proceedings 4th International Symposium of the European Foundation for Plant Pathology, 9-12 September 1996, 53-57, Bonn, Germany.
• Misawa T., Kurose D., 2018. First report of binucleate Rhizoctonia AG U causing black scurf on potato tubers in Japan. New Disease Reports, 38, 24.
• Ogoshi A., Cook R.J., Bassett E.N., 1990. Rhizoctonia species and anastomosis groups causing root rot of wheat and barley in the Pacific Northwest. Phytopathology, 80 (9), 785-788.
• Okubara P.A., Schroeder K.L., Patilitz T.C., 2008. Identification and quantification of Rhizoctonia solani and R. oryzae using real-time polymerase chain reaction. Phytopathology, 98 (7), 837-847.
• Otero J.T., Ackerman J.D., Bayman P., 2002. Diversity and host specificity of endophytic Rhizoctonia-like fungi from tropical orchids. American Journal of Botany, 89 (11),18521858. doi: 10.3732/ajb.89.11.1852
• Pascual C.B., Toda T., Raymondo A.D., Hyakumachi M., 2000. Characterization by conventional techniques and PCR of Rhizoctonia solani isolates causing banded leaf sheath blight in maize, Plant Pathology, 49 (1), 108–118. https://doi.org/10.1046/j.1365-3059.2000.00429.x
• Pope E.J., Carter D.A., 2001. Phylogenetic placement and host specificity of mycorrhizal isolates belonging to AG-6 and AG-12 in the Rhizoctonia solani species complex. Mycologia, 93 (4), 712-719. https://doi.org/10.1080/00275514.2001.12063202
• Priyatmojo A., Escopalao V.E., Tangonan N.G., Pascual C.B., Suga H., Kageyama K., Hyakumachi M., 2001. Characterization of a new subgroup of Rhizoctonia solani anastomosis group 1 (AG-1-ID), causal agent of a necrotic leaf spot on coffee. Phytopathology, 91 (11), 1054-1061.
• Roberts F.A., Sivasithamparam K., 1986. Identity and pathogenicity of Rhizoctonia spp. associated with bare patch diseases of cereals at field site in Western Australia. Netherlands Journal of Plant Pathology, 92 (5), 185-195. https://doi.org/10.1007/BF01977685
• Salazar O., Julian M.C., Hyakumachi M., Rubio V., 2000a. Phylogenetic grouping of cultural types of Rhizoctonia solani AG 2-2 based on ribosomal ITS sequences. Mycologia, 92 (3), 505–509. doi:10.1080/00275514.2000.12061186
• Salazar O., Julian M.C., Rubio V., 2000b. Primer based on specific rDNA-ITS sequences for PCR detection of Rhizoctonia solani, R. solani AG 2 subgroups and ecological types, and binucleate Rhizoctonia. Mycological Research, 104 (3), 281–285. doi:10.1017/S0953756299001355
• Sambrook J., Fritsch E.F., Maniatis T., 1989. Molecular cloning: a Laboratory manual (3 volume), 2nd Ed. Cold Spring Harbor Laboratory Press, New York.
• Sharon M., Freeman S., Kuninaga S., Sneh B., 2007. Genetic diversity, anastomosis groups, and pathogenicity of Rhizoctonia spp. isolates from strawberry. European Journal of Plant Pathology, 117 (3), 247–265.
• Sharon M., Kuninaga S., Hyakumachi M., Naito S., Sneh B., 2008. Classification of Rhizoctonia spp. using rDNA-ITS sequence analysis supports the genetic basis of the classical anastomosis grouping. Mycoscience, 49 (2), 93–114.
• Sneh B., Jabaji-Hare S., Neate S., Dijst G., 1996. Rhizoctonia species: taxonomy, molecular biology, ecology, pathology and diseases control (1st Ed.), Kluwer Academic Publishers, Dordrecht, 1-559.
• Toda T., Hyakumachi M., Suga H., Kageyama K., Tanaka A., Tani T., 1999. Differentiation of Rhizoctonia AG-D isolates from turfgrass into subgroups I and II based on rDNA and RAPD analysis. European Journal of Plant Pathology, 105, 835–846. https://doi.org/10.1023/A:1008736404263
• Toda T., Mushika T., Hyakumachi M., 2004. Development of specific PCR primers for the detection of Rhizoctonia solani AG 2-2 LP from the leaf sheaths exhibiting large-patch symptom on zoysia grass. FEMS Microbiology Letters, 232 (1), 67–74. doi: 10.1016/S0378-1097(04)00016-3
• Toda T., Mushika T., Hayakawa T., Tanaka A., Tani T., Hyakumachi M., 2005. Brown ring patch: a new disease on bentgrass caused by Waitea circinata var. circinata. Plant Disease, 89 (6), 536-542. doi: 10.1094/PD-89-0536
• Tomaso-Peterson M., Trevathan L.E., 2007. Characterization of Rhizoctonia-like fungi isolated from agronomic crops and turfgrasses in Mississippi. Plant Disease, 91 (3), 260-265. doi: 10.1094/PDIS-91-3-0260
• Ünal F., Dolar F.S., 2012. First report of Rhizoctonia solani AG 8 in Turkey. Journal of Phytopathology, 160 (1), 52–54. https://doi.org/10.1111/j.1439-0434.2011.01856.x
• Ünal F., Dolar F.S., Yıldırım A.F., Demirci E., 2014. Isolation and Identification of binucleat Rhizoctonia spp. from wheat field soils in the Central Anatolia Region, Turkey. Turkish Journal of Agricultural and Natural Sciences, 1 (2), 1933-1938.
• Ünal F., Bayraktar H., Yıldırım A.F., Akan K., Dolar F.S., 2015. Kayseri, Kırşehir, Nevşehir ve Aksaray illeri buğday ekim alanlarındaki Rhizoctonia tür ve anastomosis gruplarının belirlenmesi. Bitki Koruma Bülteni (Plant Protection Bulletin), 55 (2), 107-122.
• White T.J., Bruns T., Lee S., Taylor J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications, Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. (Eds.). Academic Press, Inc., New York. pp. 315-322.
• Yang Y.G., Zhao C., Guo Z.J., Wu X.H., 2015. Characterization of a new anastomosis group (AG-W) of binucleate Rhizoctonia, causal agent for potato stem canker. Plant Disease, 99 (12), 1757-1763. doi: 10.1094/PDIS-01-15-0036-RE.
• Zhao C., Li Y., Liu H., Li S., Han C., Wu X.A., 2019. Binucleate Rhizoctonia anastomosis group (AG-W) is the causal agent of sugar beet seedling damping-off disease in China. European Journal of Plant Pathology, 155 (1), 53–69. doi:10.1007/s10658-019-01749-4
• Zhou Q.X., Chang K.F., Hwang S.F., Strelkov S.E., Gossen B.D., Chen Y.Y., 2009. Pathogenicity and genetic diversity of Rhizoctonia solani isolates from lupin and other crops in Alberta, Canada. Canadian Journal of Plant Pathology, 31 (3), 340–347. https://doi.org/10.1080/07060660909507608