Buğdaydan elde edilen Rhizoctonia anastomosis gruplarının moleküler karakterizasyonu

Öz

Orta Anadolu buğday tarlalarından toplanan bitki ve toprak örneklerinden izole edilen Rhizoctonia izolatları MseI, Ava II/HincII, MunI, MspI (HapII), HinfI, EcoRI, HaeIII, HinfI ve HhaI enzimleri kullanılarak PCR-RFLP ve DNA sekans analizine tabi tutulmuştur. Analiz sonucunda Rhizoctonia izolatlarının çok çekirdekli Rhizoctonia solani AG 2-1, AG 2 BI, AG 3, AG 4 HG II, AG 5, AG 8 ve Waitea circinata var. circinata, Waitea circinata var. zeae, Waitea circinata var. oryzae grup I, çift çekirdekli Rhizoctonia AG DI, AG E, AG G, AG I ve AG K olduğu belirlenmiştir. Filogenetik ağaçlarda aynı grup ve altgrupta yer alan izolatlar aynı grupta yer almışlardır. Farklı grup ve alt gruptaki izolatlar ise farklı dallarda yer alarak grup ve altgruplar arasındaki genetik farklılığı ortaya koymuştur.

Anahtar Kelimeler

• Rhizoctonia
• anastomosis grup
• PCR
• RFLP
• buğday

Molecular characterization of Rhizoctonia anastomosis groups from wheat

Abstract

Rhizoctonia isolates isolated from plant and soil samples collected from Central Anatolian wheat fields were subjected to PCR-RFLP and DNA sequence analysis using MseI, Ava II/HincII, MunI, MspI (HapII), HinfI, EcoRI, HaeIII, HinfI and HhaI enzymes. As a result of the analysis, Rhizoctonia isolates were determined to be multinucleate Rhizoctonia solani AG 2-1, AG 2 BI, AG 3, AG 4 HG II, AG 5, AG 8, and Waitea circinata var. circinata, Waitea circinata var. zeae, Waitea circinata var. oryzae group I, binucleate Rhizoctonia AG DI, AG E, AG G, AG I and AG K. Isolates in the same group and subgroup in phylogenetic trees were in the same group. Isolates from different groups and subgroups were located in various branches, revealing the genetic differences between the groups and subgroups.

Keywords

• Rhizoctonia
• anastomosis group
• PCR
• RFLP
• wheat

Kaynakça

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