Turunçgil Psorosis Virüsü (CPsV)’nün Çukurova bölgesinde dağılımı ve kılıf protein genine göre moleküler olarak tanılanması

Öz

Bu çalışmada, Çukurova Bölgesi’nde Turunçgil kavlama hastalığı etmeni Citrus psorosis virus (CPsV)’nin portakal, mandarin ve altıntop bahçelerinde yaygınlığı belirlenmiş, alınan bitki örneklerinin biyolojik ve moleküler karakterizasyonu yapılmıştır. CPsV izolatlarının arasındaki farklılıklar nükleotid dizilimleri analiz edilerek belirlenmiştir. CPsV’nin tanılanması ve karakterizasyonu için 50 farklı turunçgil ağacından şiddetli kabuk kavlama lezyonu ve genç yapraklarda damar bantlaşması, meşe yaprağı deseni simptomu, yaşlı yaprak simptomları gösteren farklı izolatlar toplanmıştır. Toplanan örnekler RT-PCR çalışmalarıyla analiz edilmiştir. Kullanılan farklı primer çiftleri ile CPsV’nin kılıf protein bölgesi belirlenmiştir. Yapılan biyolojik indeksleme çalışmalarında ise tohumdan yetiştirilen Pineapple portakal (Citrus sinensis) fidanlarına CPsV’nin inokulasyonu yapılmış ve enfekteli indikatör bitkilerin ilk gelişen sürgünlerinde CPsV’nin damar bantlaşması ve şok simptomları gözlemlenmiştir. Blast analizi ve NCBI gen bankasındaki CPsV’nin referans genomlarının karşılık gelen bölgeleri ile nükleotid dizilimlerinin %98 oranında benzerliğe sahip olduğu belirlenmiştir.

Anahtar Kelimeler

• turunçgil,CPsV,RT-PCR,biyolojik indeksleme,Türkiye

Distribution and molecular detection of Citrus psorosis virus (CPsV) based on coat protein gene in Cukurova region

Abstract

This study was conducted in the Cukurova region to determine the prevalence of Citrus psorosis virus (CPsV), a citrus disease agent in orange, mandarin, and grapefruit orchards. Biological and molecular characterization of collected plant samples were also carried out and the differences between the CPsV isolates were separated by analyzing nucleotide sequences. For the identification and characterization of CPsV, isolates were collected from 50 different citrus trees showing severe barking lesions, vein banding, and oak leaf pattern symptoms on young leaves, old leaves symptoms. The leaf samples were tested by RT-PCR analysis using different primer pairs to identify the coat protein region of CPSV. For the biological indexing studies, CPsV was inoculated to Pineapple orange (Citrus sinensis) plants grown from the seed. Vein banding symptoms and shock reactions of CPsV were observed in the first growth flushes of infected indicator plants during biological indexing assays. As a result of Blast analysis, CPsV in the NCBI GenBank had 98% similarity to nucleotide sequences with corresponding regions of the reference genomes.

Keywords

• citrus,CPsV,RT-PCR,biological indexing,Turkey

Kaynakça

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