Chemical composition of Achillea millefolium L. (Asteraceae) essential oil and insecticidal effect against Sitophilus granarius (Coleoptera: Curculionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae)

Yıl 2020, , 85 - 93, 31.03.2020

Mustafa Alkan

https://doi.org/10.16955/bitkorb.674239

Cited By: 4

Öz

Stored product pests are responsible for the quality and quantitative losses worldwide in stored products. Studies on the development of plant-based pest control strategies have been increasing in recent years. In this study, the insecticidal and behavioral effect of essential oil obtained from the Achillea millefolium L. (Asteraceae) plant were measured under laboratory conditions against two main stored product pests, Sitophilus granarius (Coleoptera: Curculionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae). Contact and fumigant activity tests were conducted at 0.10 (v/v) and 0.15 (v/v) concentration, and mortality rates were recorded at the end of the 24th and 48th hours. Pure acetone was used in the control group. Repellent activity tests were established at three different concentrations (0.025 µl/cm2, 0.06 µl/cm2 and 0.125 µl/cm2) and the effects of 2nd, 4th, 8th, and 12th hours were recorded. Additionally, the essential oil composition of A. millefolium was determined by using GC-MS. For both insects, essential oil did not demonstrate significant fumigant activity. At the end of 48 hours, the highest fumigant activity was determined with a mortality rate of 19.7% for R. dominica. This oil did not show any fumigant activity against Sitophilus granarius. At the end of 24 hours, the highest contact activity was determined against R. dominica at the dose of 0.15 (v/v) with a mortality rate of 99.2%. In the same period, the contact activity was determined as 83.4% for S. granarius. Consequently, the essential oil showed significant repellent activity against both pests.

Anahtar Kelimeler

essential oil, GC-MS

Teşekkür

The author would like to thank Dr. Ünal Asav (Plant Protection Central Research Institute, Ankara, Turkey) for identifications plant metarial. The author also extends sincerest thanks to Dr. Kaan Polatoğlu (Altinbas Universtiy, School of Pharmacy, İstanbul, Turkey) for his inputs in GC-MS analysis. This study was presented as a oral presentation at the 1st International Congress on Sustainable Agriculture and Technology, April 1-3, 2019, Gaziantep

Achillea millefolium L. (Asteraceae) uçucu yağının kimyasal bileşimi ve Sitophilus granarius (Coleoptera: Curculionidae) ve Rhyzopertha dominica (Coleoptera: Bostrichidae)’ya karşı insektisidal aktivitesi

Öz

Depo zararlıları tüm dünyada depolanan ürünlerde kalite ve kantite kayıplarına neden olmaktadır. Son yıllarda zararlılarla mücadelede bitkisel temelli mücadele stratejilerinin geliştirilmelerine yönelik çalışmalar artmaktadır. Bu çalışmada, Achillea millefolium L. (Asteraceae) bitkisinden elde edilen uçucu yağın iki önemli depolanmış ürün zararlısı Sitophilus granarius (Coleoptera: Curculionidae) ve Rhyzopertha dominica (Coleoptera: Bostrichidae)'ya karşı insektisidal ve davranışsal etkileri laboratuvar ortamında test edilmiştir. Kontakt ve fumigant aktivite testleri 0,10 (v/v) ve 0,15 (v/v) konsantrasyonda kurulmuş ve 24. ve 48. saatlerin sonunda ölü bireyler kaydedilmiştir. Kontrol grubunda saf aseton kullanılmıştır. Repellent aktivite testleri üç farklı konsantrasyonda (0,025 µl/cm2, 0,06 µl/cm2 ve 0,125 µl/cm2) kurulmuş ve 2, 4, 8 ve 12 saat sonunda böceklerin tercihleri kaydedilmiştir. Ayrıca, A. millefolium'un uçucu yağ içerikleri GC-MS kullanılarak belirlenmiştir. Denemede kullanılan uçucu yağlar her iki böcek türü için kayda değer bir fumigant aktivite göstermemiştir. En yüksek fumigant aktivite, %19,7 ölüm oranıyla 48 saatin sonunda R. dominica için belirlenmiştir. Sitophilus granarius üzerinde uçucu yağ herhangi bir fumigant aktivite göstermemiştir. 24 saat sonunda en yüksek kontakt aktivite 0,15 (v/v) uygulama dozunda R. dominica’ya karşı belirlenmiş ve %99,2 ölüm oranı tespit edilmiştir. Aynı zaman diliminde S. granarius için kontakt aktivite %83,4 olarak belirlenmiştir. Bu uçucu yağ her iki zararlı içinde önemli derecede repellent aktivite göstermiştir.

Anahtar Kelimeler

Asteraceae, fumigant aktivite, kontakt aktivite, repellent aktivite, uçucu yağ

Kaynakça

• Alkan, M., Gökçe, A., 2012. Tanacetum abrotanifolium (L.) DRUCE (Asteraceae)’un gövde ve çiçek ekstraktlarının Sitophilus granarius ve Sitophilus oryzae (Col., Curculionidae)’ye olan kontakt ve davranışsal etkileri. Türkiye Entomoloji Dergisi, 36 (3), 377-389.
• Başer, K. H. C., 2009. Uçucu yağlar ve aromaterapi. Fitomed, 7, 8-25.
• Bond, E. J., 1984. Manual of fumigation for insect control. FAO Plant Production and Protection Paper 54.
• Caballero-Gallardo, K., Olivero-Verbel, J., Stashenko, E. E., 2011. Repellent activity of essential oils and some of their individual constituents against Tribolium castaneum Herbst Journal of Agricultural and Food chemistry, 59(5), 1690-1696.
• García, M., Donadel, O. J., Ardanaz, C. E., Tonn, C. E., Sosa, M. E., 2005. Toxic and repellent effects of Baccharis salicifolia essential oil on Tribolium castaneum. Pest Management Science: formerly Pesticide Science, 61(6), 612-618.
• Gökce¸ A., Whalon, M. E., Cam, H., Yanar, Y., Demirtas, I., GOREN, N., 2007. Contact and Residual Toxicities of 30 Plant Extracts to Colorado Potato Beetle Larvae. Archives of Phytopathology and Plant Protection, 149(2), 1-10.
• Güncan, A., Durmuşoğlu, E., 2004. Bitkisel kökenli doğal insektisitler üzerine bir değrlendirme. Hasad dergisi, 233, 26-32.
• Isman, M.B., Grieneisen, M.L. 2014. Botanical insecticide research: many publications, limited useful data. Trends in plant science, 19(3), 140-145.
• Juliana. G., Su, H.C.F., 1983. Laboratory studies on several plant materials as insect repellents for protection of cereal grains. Journal of Economic Entomology, 76, 154-157.
• Kim, S. I., Park, C., Ohh, M.H., Cho, H.C., Ahn, Y.J., 2003. Contact and fumigant activities of aromatic plant extracts and essential oils against Lasioderma serricorne (Coleoptera: Anobiidae). Journal of Stored Products Research, 39(1), 11-19.
• Kordali, S., Memis, K., Cakir, A., 2007. Toxicity of monoterpenes against larvae and adults of Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Industrial Crops and Products, 26, 278-297
• Liu, C. H., Mishra, A.K., Tan, R.X., Tang, C., Yang, H., Shen, Y.F., 2006. Repellent and insecticidal activities of essential oils from Artemisia princeps and Cinnamomum camphora and their effect on seed germination of wheat and broad bean. Bioresource technology, 97(15), 1969-197
• McDonald, L.L.. Guy, R.H., Speirs, R.D., 1970. Preliminary evaluation of new candidate materials as toxicants, repellents and attractants against stored product insect. Marketing research report no.882. Agricultural Research Service. US Deparment of Agriculture. Washington. 183 pp.
• Mutungi, C., Affognon, H., Njoroge, A., Baributsa, D., Murdock, L., 2014. Storage of mung bean (Vigna radiata[L.] Wilczek) and pigeonpea grains (Cajanus cajan [L.] Millsp) in hermetic triple-layer bags stops losses caused by Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Journal of Stored Product Research, 58, 39–47.
• Nerio, L. S., Olivero-Verbel, J., Stashenko, E.E., 2009. Repellent activity of essential oils from seven aromatic plants grown in Colombia against Sitophilus zeamais Motschulsky (Coleoptera). Journal of Stored Products Research, 45(3), 212-214.
• Obeng-Ofori, D., Reichmuth, C.H., Bekele, J., Hassanali, A., 1997. Biological activity of 1, 8 cineole, a major component of essential oil of Ocimum kenyense (Ayobangira) against stored product beetles. Journal of Applied Entomology, 121(1-5): 237-243.
• Papachristos, D.P., Karamanoli, K.I., Stamopoulos, D.C., Menkissoglu‐Spiroudi, U., 2004. The relationship between the chemical composition of three essential oils and their insecticidal activity against Acanthoscelides obtectus (Say). Pest Management Science: formerly Pesticide Science, 60(5), 514-520.
• Polatoğlu, K., Karakoç, Ö. C., Gören, N., 2013. Phytotoxic. DPPH scavenging. insecticidal activities and essential oil composition of Achillea vermicularis. A. teretifolia and proposed chemotypes of A. biebersteinii (Asteraceae). Industrial crops and products, 51, 35-45.
• Shanker, C., Solanki, K.R., 2000. Botanical insecticides: A historical perspective. India. Asian Agrihistory, 4(2), 21-30.
• Taiz, L., Zeiger, E., 2002. Plant Physiology. Sinauer Associates, Sunderland, Massachusetts, 603 pp
• Taylor, R.W.D., 1994. Methyl bromide- Is there any future for this noteworthy fumigant? Journal of Stored Products Research, 30, 253-260.
• Wang, J., Zhu, F., Zhou, X.M., Niu, C. Y., Lei, C.L., 2006. Repellent and fumigant activity of essential oil from Artemisia vulgaris to Tribolium castaneum (Herbst)(Coleoptera: Tenebrionidae). Journal of Stored Products Research, 42(3), 339-347.