Determination of the insecticide resistance mechanisms by biochemical assays in <em>Myzus persicae</em> (Sulz.) (Hemiptera:Aphididae) populations collected from Icel-Turkey

Yıl 2008, Cilt: 48 Sayı: 3, 15 - 31, 10.11.2009

A. Velioğlu G. Moores A. Devonshıre S. Toros

Öz

The carboxylesterase correlated with insecticide resistance in different Myzus persicae populations collected from Icel were analysed by using microplate reader and electrophoresis. Total carboxylesterase activities and carboxylesterase E4 band patterns of the populations were determined and the values were found as extremely high levels at all of Icel populations. Results of the detailed analyses from microplate assay, immunoassay and electrophoresis showed that Icel populations have very high levels and FE4 type of carboxylesterase.

Icel populations were also examined the case of insecticide resistance resulted from insensitivity of acetylcholinesterase (AChE). It was found that three Icel populations have insensitive AChE to pirimicarb. Icel populations showed “multiple resistance mechanismâ€?, because of high carboxylesterase activities and also insensitive AChE.

Anahtar Kelimeler

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İçel’den toplanan Myzus persicae (Sulz.) (Hemiptera: Aphididae) popülasyonlarında insektisitlere direnç mekanizmalarının biyokimyasal yöntemlerle belirlenmesi

Öz

Anahtar Kelimeler

Yeşil şeftali yaprakbiti, insektisit direnci, karboksilesteraz, asetilkolinesteraz, immunoassay, PAGE

Kaynakça

• Anonymous, 2008. http://www.rothamsted.ac.uk/insect-survey/STMyzus_persicae.php.
• Anstead, J. A., Williamson, M. S., Eleftherianos, I. and Denholm, I. 2004. High-throughput detection of knockdown resistance in Myzus persicae using allelic discriminating quantitative PCR. Insect Biochemistry and Molecular Biology, 34, 871–877.
• Bizzaro, D., Mazzoni, E., Barbolini, E., Giannini, S., Cassanelli, S., Pavesi, F., Cravedi, P. and Manicardi, G. C. 2005. Relationship among expression, amplification, and methylation of FE4 esterase genes in Italian populations of Myzus persicae (Sulzer) (Homoptera: Aphididae). Pesticide Biochemistry and Physiology, 81, 5158.
• Criniti, A., Mazzoni, E., Cassanelli, S., Cravedi, P., Tondelli, A., Bizzaro, D. and Manicardi, G. C. 2008. Biochemical and molecular diagnosis of insecticide resistance conferred by esterase, MACE, kdr and super- kdr based mechanisms in Italian strains of the peach potato aphid, Myzus persicae (Sulzer). Pesticide Biochemistry and Physiology, 90, 168-174.
• Devine, G. and Devonshire, A. L. 1994. Immunoassay protocol for characterising insecticide-resistant Myzus persicae. 5 p., (basılmamış laboratuvar protokolu).
• Devonshire, A. L. 1975. Studies of the carboxylesterases of Myzus persicae resistant and susceptible to organophosphorus insecticides. Proceedings 8th British Insecticide and Fungicide Conference, 67-73.
• Devonshire, A. L. 1977. The properties of a carboxylesterase from the peach-potato aphid, Myzus persicae (Sulz.), and its role in conferring insecticide resistance. Biochem. J., 167, 675-683.
• Devonshire, A. L. and Moores, G. D. 1982. A carboxylesterase with broad substrate specificity causes organophosphorus, carbamate and pyrethroid resistance in peach-potato aphids (Myzus persicae). Pesticide Biochemistry and Physiology, 18, 235-246.
• Devonshire, A. L., Moores, G. D. and Chiang, C. L. 1983. The biochemistry of insecticide resistance in the peach-potato aphid, Myzus persicae. In: Pesticide Chemistry: Human Welfare and the Environment (eds. Miyamoto, J., Kearney, P. C., Matsunaka, D. H. and Murphy, S. D.). Proceedings of the 5th International Congress of Pesticide Chemistry, 191-196, Pergamon, Oxford.
• Devonshire, A. L. and Moores, G. D. 1984. Immunoassay of carboxylesterase activity for identifying insecticide resistant Myzus persicae. British Crop Protection Conference-Pests and Diseases, 515- 520.
• Devonshire, A. L., Moores, G. D. and Ffrench-Constant, R. H. 1986. Detection of insecticide resistance by immunological estimation of carboxylesterase activity in Myzus persicae (Sulzer) and cross reaction of the antiserum with Phorodon humuli (Schrank) (Hemiptera: Aphididae). Bull. ent. Res., 76, 97- 107.
• Devonshire, A. L. 1989a. Insecticide resistance in Myzus persicae : from field to gene and back again. Pestic. Sci. 26, 375-382.
• Devonshire, A. L. 1989b. The role of electrophoresis in the biochemical detection of insecticide resistance. In: Electrophoretic Studies on Agricultural Pests (eds. Loxdale, H. D. and Hollander, J. den). Systematic Association Special Volume No: 39, Clarendon Press, 363-374, Oxford.
• Devonshire, A. L. 1991. Role of esterases in resistance of insects to insecticides. Biochemical Society Transactions, 19 (3), 755-759.
• Devonshire, A. L. and Field, L. M. 1991. Gene amplification and insecticide resistance. Annu. Rev. Entomol., 36, 1-23.
• Devonshire, A. L., Devine, G. J. and Moores, G. D. 1992. Comparison of microplate esterase assays and immunoassay for identifying insecticide resistant variants of Myzus persicae (Homoptera: Aphididae). Bull. ent. Res., 82, 459-463.
• Ffrench-Constant, R. H. and Devonshire, A. L. 1987. A multiple homogenizer for rapid sample preparation in immunoassays and electrophoresis. Biochem. Genet., 25, 493- 499.
• Ffrench-Constant, R. H., Devonshire, A. L. and Clark, S. J. 1987. Differential rate of selection for resistance by carbamate, organophosphorus and combined pyrethroid and organophosphorus insecticides in Myzus persicae (Sulzer) (Hemiptera: Aphididae). Bull. ent. Res., 77, 227-238.
• Ffrench-Constant, R. H. and Devonshire, A. L. 1988. Monitoring frequencies of insecticide resistance in Myzus persicae (Sulzer) (Hemiptera: Aphididae) in England during 1985-86 by immunoassay. Bull. ent. Res., 78, 163-171.
• Ffrench-Constant, R. H., Devonshire, A. L. and White, R. P. 1988. Spontaneous loss and reselection of resistance in extremely resistant Myzus persicae (Sulzer). Pestic. Biochem. Physiol., 30, 1-10.
• Field, L. M. and Devonshire, A. L. 1992. Esterase genes conferring insecticide resistance in aphids. In: Molecular Mechanisms of Insecticide Resistance: Diversity Among Insects (eds. Mullin, C. A. and Scott, J. G.). American Chemical Society, 209-217, USA.
• Field, L. M., Williamson, M. S., Moores, G. D. and Devonshire, A. L. 1993. Cloning and analysis of the esterase genes conferring insecticide resistance in the peach-potato aphid, Myzus persicae (Sulzer). Biochem. J., 294, 569-574.
• Field, L. M. and Devonshire, A. L. 1997. Structure and organization of amplicons containing the E4 esterase genes responsible for insecticide resistance in the aphid Myzus persicae (Sulzer). Biochem. J., 322, 867-871.
• Field, L. M. and Foster, S. P. 2002. Amplified esterase genes and their relationship with other insecticide resistance mechanisms in English field populations of the aphid, Myzus persicae (Sulzer). Pest Management Science 58, 889–894.
• Foster, S. P., Denholm, I., Harling, Z. K., Moores, G. D. and Devonshire, A. L. 1998. Intensification of insecticide resistance in UK field populations of the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae) in 1996. Bull. ent. Res., 88, 127130.
• Mazzoni, E. and Cravedi, P. 2002. Analysis of insecticide-resistant Myzus persicae (Sulzer) populations collected in Italian peach orchards. Pest Management Science 58, 975–980.
• Metcalf, R. L. 1989. Insect resistance to insecticides. Pestic. Sci., 26, 333-358.
• Moores, G. D., Denholm, I., Byrne, F. J., Kennedy, A. L. and Devonshire, A. L. 1988. Characterising acetylcholinesterase genotypes in resistant insect populations. Brighton Crop Protection Conference-Pests and Diseases, 451-456.
• Moores, G. D., Ffrench-Constant, R. H. and Devonshire, A. L. 1989. Immunoassay for detecting insecticide resistance in aphids. Pestic. Sci., 26, 324-326.
• Moores, G. D., Devine, G. J. and Devonshire, A. L. 1994a. Insecticide-insensitive acetylcholinesterase can enhance esterase-based resistance in Myzus persicae and Myzus nicotianae. Pesticide Biochemistry and Physiology, 49, 114-10.
• Moores, G. D., Devine, G. J. and Devonshire, A. L. 1994b. Insecticide resistance due to insensitive acetylcholinesterase in Myzus persicae and Myzus nicotianae. Brighton Crop Protection Conference-Pests and Diseases, 413-418.
• Moores, G. D. 1995. New resistant-aphid threat from abroad. Arable Farming, 22 (7), 1013.
• Moores, G. D., Gao, X., Denholm, I. and Devonshire, A. L. 1996a. Characterisation of insensitive acetylcholinesterase in insecticide-resistant cotton aphids, Aphis gossypii Glover (Homoptera: Aphididae). Pesticide Biochemistry and Physiology, 56, 102-110.
• Moores, G. D., Han, Z. J., Denholm, I. and Devonshire, A. L. 1996b. Two forms of insecticide insensitive acetylcholinesterase in Aphis gossypii. Brighton Crop Protection Conference-Pests and Diseases,745-750.
• Ornstein, L. and Davis, B. J. 1964. Disc electrophoresis I. Background and theory. Ann. NY. Acad. Sci., 121, 321-349.
• Toutant, J. P. 1989. Insect acetylcholinesterase: catalytic properties, tissue distribution and molecular forms. Progress in Neurobiology, 32, 423-446.
• Velioğlu, A. S. ve Toros S. 2002. Değişik bölgelerden toplanan Myzus persicae (Sulz.) (Homoptera: Aphididae) popülasyonlarının farklı gruptan bazı insektisitlere karşı duyarlılık farklarının belirlenmesi. Bitki Koruma Bülteni, 42 (1-4): 67-79.
• Velioğlu, A. S. ve Toros S. 2006. Myzus persicae (Sulz.) (Hemiptera: Aphididae)’de insektisitlere direnç ile ilişkili karboksilesterazın spektrofotometre ve elektroforez ile belirlenmesi. Bitki Koruma Bülteni, 46 (1-4): 1-12.