Investigation of the effects of some plant extracts and chemical substances on the infection of <em>Tobacco mosaic virus</em>

Abstract

In the present study, the inhibitory effects of 14 plant extracts and 15 chemical substances were examined on the infection of Tobacco mosaic virus (TMV). According to the results of experiments on test plants, plant extracts inhibited virus infection at the ratios of 25.92 % to 97.19 % while the inhibition levels of chemical substances on virus infection ranged between 10.24 % and 98.30 %. The findings showed that the highest inhibition levels were obtained with three plant extracts (Dianthus caryophyllus, Capsicum annuum and Yucca elephantipes) and three chemicals (Sodium dodecylsulfate, Triton X-100 and HuwaSan). Therefore, the mentioned extracts and chemicals were selected for further steps of the study at which the effects of some factors as dilution, temperature and application times on the inhibition of virus infection were examined. Considering the data from assays on test plants, it was observed that inhibitory activity of D. caryophyllus and sodium dodecylsulfate was not affected much more with dilution and temperature fluctuations. Moreover, the results showed that extract from D. caryophyllus had more inhibitory effect when it was applied in 2 and 4 hours before virus inoculation and on the contrary Sodium dodecylsulfate inhibited virus infection at higher level when used in 2 and 4 hours after virus application. Later, in pot experiments performed on tobacco (Nicotiana tabacum L. cv. Akhisar 97) plants, it was observed that the application of D. caryophyllus extract and reduction of virus concentration in the tobacco plants by spreying sodium dodecylsulfate before or after virus inoculation was determined by results of DAS-ELISA test.

Keywords

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Bazı bitki ekstraktları ve kimyasal maddelerin tütün mozaik virüsü enfeksiyonuna etkilerinin araştırılması

Öz

Anahtar Kelimeler

• Tütün, tütün mozaik virüsü, engelleme, bitki ekstraktı, kimyasal madde.

Kaynakça

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