Survey for Grapevine leafroll viruses (GLRaVs) in Malatya region

Abstract

Grapevine (Vitis vinifera L.) is one of the most important fruit crops in Turkey and viral diseases are one of the main problems of grapevine production. They decrease yield quantity and quality of crops as well as cause death of the plants in an interaction with other agents. Leafroll disease of grapevine, caused by a complex of viruses, is considered a serious threat to production across many grapevine growing regions in Turkey. This study was conducted to determine the current status of Grapevine leafroll-associated viruses (GLRaVs) in Malatya province. Grapevine plantations in Malatya province were surveyed for the presence of Grapevine leafroll-associated viruses (GLRaVs) -1, -2, -3, -4 and -6. Purposive sampling was done in vineyards of Arapgir, Darende and Battalgazi districts, which are the primary grapevine producing areas of Malatya during 2016-2017 growing seasons. A total of 241 samples were collected and tested by DAS-ELISA. Samples giving two fold of absorbance values (405 nm) of negative control were evaluated as positive. According to the results, 131 samples were found to be infected with GLRaV-1, whereas 48 samples with GLRaV-3 and -4 samples with GLRaV-4. None of the samples were reacted as positive against GLRaV-2 and GLRaV-6. Forty five samples were found to be infected with mix infection of GLRaV-1 and -3, and three samples were found to be infected with GLRaV-1 and -4 strain 6.

Keywords

• Survey
• grapevine
• Grapevine leafroll associated viruses
• Malatya

Malatya bölgesi bağlarında Asma yaprak kıvrılma virüsleri (GLRaVs)’nin sürveyi

Öz

Asma (Vitis vinifera L.) ülkemizde yetiştiriciliği yapılan en önemli meyvelerden birisi olup üretimini sınırlayan faktörlerin başında virüs hastalıklar gelmektedir. Virüs hastalıkları üzüm üretimini kalite ve kantite bakımından azaltmanın yanı sıra diğer hastalık etmenleri ile beraber bulunmaları durumunda asmanın kurumasına kadar ulaşabilen ciddi sonuçlar doğurabilmektedir. Bir virüs kompleksinin neden olduğu Asma yaprak kıvrılma hastalığı ülkemiz asma yetiştiriciliği üzerinde önemli bir tehdittir. Bu çalışma, Malatya ilinde yetiştirilen asmaları etkileyen Asma yaprak kıvırcıklık virüsleri (Grapevine leafroll associated virus, GLRaV)’ini tespit etmek amacı ile yürütülmüştür. Bu amaçla Malatya ilindeki asma bahçeleri gezilerek etmenin 1, 2, 3, 4 ve 6 nolu ırkları araştırılmıştır. 2016-2017 yıllarında Arapgir, Darende ve Battalgazi ilçelerini kapsayan survey çalışmalarında güdümlü örneklemeler yapılarak asma numuneleri toplanmıştır. Toplanan 241 örneğin tamamı DAS-ELSA metodu ile testlenmiştir. Testlerde negatif örneğin iki katı ve yukarısında absorbans (405 nm) değeri veren numuneler infekteli kabul edilmiştir. Yürütülen test sonuçlarına göre toplam 131 numunenin GLRaV 1 ile, 48 numunenin GLRaV 3 ile ve 4 örneğin ise GLRaV 4 ile infekteli olduğu tespit edilmiştir. Örneklerin hiçbirinde GLRaV 2 ve GLRaV 6 infeksiyonuna rastlanmamıştır. Testlenen 45 numunenin GLRaV 1 ve 3 ile, üç numunenin ise GLRaV 1 ve 4 ile karışık infeksiyon halinde bulunduğu tespit edilmiştir. 

Anahtar Kelimeler

• Survey
• grapevine
• Grapevine leafroll associated viruses
• Malatya

Kaynakça

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