Origanum onites L. uçucu yağının kimyasal bileşenleri ve allelopatik etkisi

Yıl 2019, Cilt: 59 Sayı: 3, 71 - 78, 30.09.2019

Melih Yılar , Yusuf Bayar , Abdurrahman Onaran

https://doi.org/10.16955/bitkorb.542301

Cited By: 4

Öz

Bu çalışmada, Origanum
onites L. bitkisinin toprak üstü (sürgün+yaprak+çiçek)
aksamından elde edilen uçucu yağın kimyasal bileşenleri ve farklı bitkilerin
çimlenme ve fide gelişimine olan allelopatik etkileri araştırılmıştır. Bu
amaçla Mersin ilinden toplanan O.
onites bitkisinden hidro-distilasyon yöntemi kullanılarak uçucu yağ
elde edilmiştir. GC/MS analizi sonucunda O.
onites uçucu yağında carvacrol (%59.87), γ-terpinene (%17.08) ve β-cymene (%8.83) temel bileşenler
olmak üzere 24 bileşen belirlenmiştir. Allelopatik etki 9 cm çaplı Petri kaplarında
yürütülmüştür. Petri kaplarına 2 kat halinde kurutma kağıdı yerleştirilmiş ve Amaranthus
retroflexus L., Lepidium sativum L., Triticum aestivum L. tohumları homojen olarak dağıtılmıştır.
Kurutma kağıtları distile su kullanılarak iyice nemlendirilmiştir. Her bir Petri
kabının kapağının merkezine zamkla yapıştırılmış kurutma kağıdına uçucu yağlar
farklı konsantrasyonlarda (0, 0.5, 1, 1.5, 5, 15 μl/Petri) bir mikropipet
yardımı ile damlatılarak, Petri kapları örtülerek Parafilm ile sıkıca
sarılmıştır. Petri kapları 12 saat aydınlık-12 saat karanlık ve ortalama 24±1 ^oC
koşullarda 1-4 hafta boyunca inkübasyona bırakılmıştır. Sürenin sonunda
çimlenme oranı, kök ve sürgün boyları belirlenmiştir. O. onites uçucu yağı A.
retroflexus L., L.
sativum L., T. aestivum L. bitkilerinin tohum
çimlenmesi ile kök ve sürgün gelişimini 15 μl/Petri dozda tamamen
engellemiştir. Uçucu yağa karşı A. retroflexus L.’un daha hassas olduğu tespit edilmiştir. Elde edilen sonuçlara göre,
O. onites uçucu yağının yüksek seviyede L.
sativum L., T. aestivum L., A. retroflexus L. tohum
çimlenmesi ve fide gelişimleri üzerine allelopatik etki gösterdiği ve A.
retroflexus L. kontrolünde kullanılabilir potansiyele sahip olduğu
belirlenmiştir.

Anahtar Kelimeler

allelopatik etki, Origanum onites, uçucu yağ

Chemical composition and allelopathic effect of Origanum onites L. essential oil

Öz

In this study, chemical composition and allelopathic effect of essential oil obtained from ground parts (shoots+leaves+flowers) of Origanum onites L. plant on seed germination and seedling growth of different plant species were investigated. Essential oil was obtained with the use of the hydro-distillation method from O. onites plant collected from Mersin province. It was identified 24 different compounds by GC/MS analysis in O. onites essential oil, while the main compounds were determined as carvacrol (59.87%), γ-terpinene (17.08%) and β-cymene (8.83%). The allelopathic effect of the essential oil, two layers of filter paper were placed bottom of 9 cm diameter disposable Petri dishes then seeds of Amaranthus retroflexus L., Triticum aestivum L. and Lepidium sativum L. were homogeneously distributed on filter paper. Filter papers were thoroughly moistened using distilled water. The filter paper was glued to the center of the lid of each Petri dish. The different concentrations (0, 0.5, 1, 1.5, 5, 15 μl/Petri dishes) of the essential oil were applied to the filter paper pieces. Then lid of each Petri dish was closed and sealed with Parafilm. Petri dishes were incubation at 12 h dark-12 h light periods with an average temperature of 24±1 ^oC for 1-4 weeks. At the end of incubation period, germination rates, root and shoot lengths of the test plants were determined. The 15 μl/Petri concentration of O. onites essential oil were completely inhibited seed germination, root and shoot growth of A. retroflexus L., T. aestivum L. and L. sativum L. plants. A. retroflexus L. was found to be more sensitive to essential oil. The results show that O. onites essential oil has potential to be used in the control of A. retroflexus L., which has high allelopathic effect on seed germination and seedling development of L. sativum L., T. aestivum L., A. retroflexus L.

Anahtar Kelimeler

allelopathic effect, Origanum onites, essential oil

Kaynakça

• Kong C.H., Zhao H., Xu X.H., Wang P., Gu Y. 2007. Activity and allelopathy of soil of flavone O-glycosides from rice. J Agric Food Chem. 55:6007–6012.
• Joshi N., Joshi A. 2016. Allelopathic effects of weed extracts on germination of wheat. Annals of Plant Sciences 5.5: 1330-1334.
• Alam S.M., Islam E. 2002. Effects of aqueous extract of leaf, stem and root of nettleleaf goosefoot and NaCl on germination and seedling growth of rice. Pak J Seed Technol 1: 47-52.
• Bajalan I., Zand M., Rezaee S. 2013. Allelopathic effects of aqueous extract from Salvia officinalis L. on seed germination of Barley and Purslane. International Journal of Agriculture and Crop Sciences, 5(7), 802-805.
• Zeng R.S. Mallik A.U., Luo S.M. 2008. Allelopathy in Sustainable Agriculture and Forestry; Springer Science+Business Media, LLC, New York, USA.
• Harley R.M., Atkins S., Budantsev A., Cantino P.D., Conn B.J., Grayer R., Harley M.M., de Kok R., Krestovskaja T., Morales R., Paton A.J., Ryding O., Upson T. 2004. Labiatae. In: Kubitzki, K. (ed.), The Families and Genera of Vascular Plants, vol. 7, pp. 167-275. Springer-Verlag, Berlin.
• Kaya A., Doğu, S., Dinç, M., Kürkçüoğlu, M. 2017. Comparison of essential oils of endemic Salvia dichroantha Stapf collected from Konya. Int. J. Sec. Metabolite, 4(3):412-417.
• Cantino P.D., Harley R.M., Wagstaff S.J. 1992. Genera of Lamiaceae: status and classification. In: Harley, R.M. & Reynolds,T.eds, Advances in Labiate Science: 511Â-522. Kew: Royal Botanic Gardens.
• Koyuncu O., Yaylacı Ö.K., Öztürk D., Erkara İ.P., Savaroğlu F., Akçoşkun Ö., Ardıç M. 2010. Risk categories and ethnobotanical features of the Lamiaceae taxa growing naturally in Osmaneli (Bilecik/Turkey) and environs. Biological Diversity and Conservation, 3(3): 31-45.
• Belen V. 2012. Salvia pilifera Montbret & Aucher ex. Bentham collected from different areas populations variations and analysis of the essential oil components. (Master tesis). Kahramanmaras Sutcu İmam University. Institute for Graduate Studies in Science and Technology, Department of Biyology, Kahramanmaras.
• Kahraman A., Celep F., Doğan M. 2009. Morphoogy, anatomy and palynology of Salvia indica L.(Labiatae). World Applied Sciences Journal, 6 (2), 289-296.
• Aydin S., Ozturk Y., Beis R., Baser K.H.C.1998. Investigation of Origanum onites, Sideritis congesta and Satureja cuneifolia essential oils for analgesic activity. Phytotherapy Research. 10(4):342-344.
• Dundar E, Olgun E. G., Isiksoy S., Kurkcuoglu M., Baser K.H.C., Bal C. 2008. The effects of intra-rectal and intra-peritoneal application of Origanum onites L. Essential oil on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat. Experimental and Toxicologic Pathology, 59:399–408.
• Kokkini S. 1996. Taxonomy, Diversity and Distribution of Origanum Species. In: Padulosi S., ed. Oregano. Proceedings of The IPGIR Int. Workshop on Oregano, 8- 12 May 1996, Valenzano (Bari), Italy. Rome: IPGRI, p.2- 12.
• Olivier G.W. 1996. The world market of oregano. p. 141–145. In: S. Padulosi, (Eds.). Oregano. Promoting the conservation and use of underutilized and neglected crops. Proceedings of the IPGRI International Workshop on Oregano. Institute of Plant Genetics and Crop Plant Research, Gater-sleben/International Plant Genetic Resources Institute, Valenzano, Italy.
• Barreyro R, Ringuelet J., Agrícola S. 2005. Nitrogen fertilization and yield in oregan (Origanum xapplii). Ciencia e Investigación Agraria, 32(1):34-38.
• Baser K.H.C., Özek T., Tümen G., Sezik E. 1993. Composition of the essential Oils of Turkish Origanum Species with Commercial Inportance. J. Essential Oil Res. 5(6): 619-623.
• Kirimer N., Boydag I., Sargin N., Arslandere O. 2003. Trading Configuration of Origanum Species in Nature. TUBITAK-TBAG-C. Sek. 10 Project (101T012).
• Toncer O., Karaman S., Kızıl S., Diraz E. 2009. Changes in Essential Oil of Oregano(Origanum onites L.) due to Diurnal Variations at Different Development Stages. Not. Bot. Hort. Agrobot. Cluj 37(2):177-181.
• Yaldiz G, Sekeroglu N., Ozgüven M., Kirpik M. 2005. Seasonal and diurnal variability of essential oil and its components in Origanum onites L. grown in the ecological conditions of Cukurova. Grasas y Aceites, 56(4):254-258.
• Lee S.O., Choi G.J., Jang K.S., Lim, H.K., Cho K.Y., Kim J.C. 2007. Antifungal activity of five plant essential oils as fumigant against postharvest and soilborne plant pathogenic fungi. Plant Pathol. J., 23(2), 97-102.
• Baruah P., Sharma R.K., Singh R.S., Ghosh A.C. 1996. Fungicidal Activity of Some Naturally Occurring Essential Oils Against Fusarium Moniliforme. Journal of Essential Oil Research, 8(4):411-412.
• Vagi E., Simandi B, Suhajda A., Hethelyi E. 2005. Essential oil composition and antimicrobial activity of Origanum majorana L. extracts obtained with ethyl alcohol and super critical carbondioxide. Food. Res. Int. 38:51-57.
• Tepe B., Daferera D., Sökmen M., Polissiou M., Sökmen A. 2004. The in vitro antioxidant and antimicrobial activities of the essential oil and various extracts of Origanum syriacum L. var. bevanii. J. Sci. Food Agric. 84:1389-1396.
• Muller R.F., Berger B., Yegen O. 1995. Chemical composition and fungitoxic properties to phytopathogenic fungi of essential oils of selected aromatic plants growing wild in Turkey. J. Agric. Food Chem. 43: 2262-2266.
• Wilson C.L., Solar J.M., El Ghaouth A., Wisniewski M.E. 1997. Rapid evaluation of plant extract and essential oils for antifungal activity against Botrytis cinerea. Plant Dis. 81:204-210.
• Traboulsi A.F., Taoubi K., El-Haj S., Bessiere J.M., Rammal S. 2002. Insecticidal properties of essential plant oils against the mosquito Culex pipiens molestus(Diptera: Culicidae). Pest Manag.Sci., 58:491-495.
• Rus C.F., Pop G., Alexa E., Sumalan, R.M., Copolovici M. 2015. Antifungal activity and chemical composition of Salvia officinalis L. essential oil. Res. J. Agric. Sci.,47:186–194.
• García-Beltrán J.M., Esteban M.A. 2015. Properties and Application of Plants of Origanum sp. Genus. SM J. Biol. 2(1),1006, page:9.
• Atak M., Mavi K., Uremiş I. 2016. Bio-Herbicidal effects of Oregano and Rosemary essential Oils on Germination and Seedling Growth of Bread Wheat Cultivars and Weeds. Romanian Biotechnological Letters, 21(1):11149-11159.
• Önen H., Özer Z., Telci İ. 2002. Bioherbicidal effects of some plant essential oils on different weed species, Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz. Journal of Plant Diseases and Protection, Sonderheft XVIII, 597-605.
• Kadioglu I. 2004. Effects of Hearleaf Cocklebur (Xanthium strumarium L.) Extract on some crops and weeds. Asian Journal of Plant Sciences. 3 (6): 696-700.
• Kordali Ş., Çakır A., Akcin T.A., Mete E., Akcin A., Aydın T., Kılıç H. 2009. Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Industrial Crops and Products, 29, 562-570.
• Sadeghi S., Rahnavard A., Ashrafi Z.Y. 2010. Allelopathic effects of Helianthus annuus (Sunflower) on Solanum nigrum (Black Nightshade) seed germination and growth in laboratuary condition. Journal of Horticultural Science & Ornamental Plants, 2(1), 32-37.
• Özkan A., Erdoğan A. 2011. A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turk J Biol 35:735-742.
• Altıntas A., Tabanca N., Tyıhak E., Ott P.G., Móricz Á.M., Mincsovics E., Wedge D.E. 2013. Characterization of Volatile Constituents from Origanum onites and Their Antifungal and Antibacterial Activity. Journal of AOAC International, 96(3):1200-1208.
• Copur G., Arslan M., Duru M., Baylan M., Canogulları S., Aksan E. 2010. Use of oregano (Origanum onites L.) essential oil as hatching egg disinfectant. African Journal of Biotechnology Vol. 8 (17), pp. 2531-2538.
• Economou G., Panagopoulos G., Tarantilis P., Kalivas D., Kotoulas V., Travlos I.S., Polysiou M., Karamanos A. 2011. Variability in essential oil content and composition of Origanum hirtum L., Origanum onites L., Coridothymus capitatus (L.) and Satureja thymbra L. populations from the Greek island Ikaria. Industrial Crops and Products 33: 236–241.
• Barney J., Hay A.G., Weston L.A. 2005. Isolation and Characterization of Allelopathic Volatiles from Mugwort (Artemisia vulgaris). Journal of Chemical Ecology,31(2):247-265.
• Erbaş S., Karaman R., Baydar H., Şanlı A. 2015. Allelopathic effect of oregano (Origanum onites L.) on germination and seedling development of some weed and cultivars. Scientific Papers. Series A. Agronomy, Vol. LVIII:190-194.
• Altindal N., Altindal D. 2013. Allelopathic Effect of sage and Turkish Oregano Volatile Oils on In Vitro in Sainfoin (Onobrychis viciifolia). Teop, 16(3):328-333.
• Azirak S., Karaman S. 2008. Allelopathic effect of some essential oils and components on germination of weed species. Acta Agriculturae Scandinavica Section B - Soil and Plant Science, 58: 88-92.
• Kordali S., Cakir A., Sutay S. 2007. Inhibitory effects of monoterpenes on seed germination and seedling growth. Z Naturforsch C. 62(3-4):207-214.
• Dudai N., Poljakoff-Mayber A., Mayer A.M., Putievsky E., Lerner H.R. 1999. Essential oils as allelochemicals and their potential use as bioherbicides. J. Chem. Ecol. 25, 1079–1089.
• Gitsopoulus T., Chatzopoulou P., Georgoulas I. 2013. Effects of essentail oils of Lavandula x hybrida Rev, Foeniculum vulgare Mill and Thymus capitatus L. on the germination and radical lenght of Triticum aestivum L., Hordeum vulgare L., Lolium rigidum L. and Phalaris brachystachys L. Journal of Essential Oil Bearing Plants,16 (6): 817-825.