Prangos pabularia Bitkisinin Antioksidan ve Antimikrobiyal Özelliklerinin Belirlenmesi

Abstract

Tıbbi ve aromatik bitkiler, tarih öncesi çağlardan bu yana tedavi edici özelliklerinin yanında gıda üretiminde tat ve aroma katmak amacıyla yaygın olarak kullanılmaktadır. Son yıllarda özellikle yurt dışında büyük ilgi gören bitkilerle tedavi yöntemi, yurdumuzda da önem kazanmaya başlamıştır. Sunulan çalışma Bitlis ilinde doğal olarak yetişen Prangos pabularia bitkisinin antioksidan ve antimikrobiyal özelliklerini incelemek amacıyla planlandı. Çalışmada kullanılan P. pabularia bitki örnekleri Bitlis Kambos Dağı’ndan toplandı. Bitkinin yaprak ve meyvelerinin uçucu yağları su distilasyonu ile elde edildi. Antioksidan özelliklerinin belirlenmesi için DPPH metodu kullanıldı. Bitki meyvelerinin ve yapraklarının antioksidan aktiviteleri sırasıyla %28.70 ve %25.54, IC₅₀ değerleri ise 15.43 µg/ml ve 16.73 µg/ml olarak saptandı. Antimikrobiyal aktivite ise disk difüzyon metodu kullanılarak belirlendi. Bitki meyvelerinden elde edilen uçucu yağlar Proteus mirabilis ve Enterobacter aurogenes standart suşlarına karşı zayıf antibiyotik etki gösterdi. Yapraklardan elde edilen uçucu yağlar ise bakteri ya da mantar suşlarına karşı herhangi bir antibiyotik etki göstermedi. 

Keywords

• Prangos pabularia,Apiaceae,Antioksidan,Antimikrobiyal

Detecting of Antioxidant and Antimicrobial Specifications of Prangos Pabularia

Abstract

Medicinal and aromatic plants have been used extensively since prehistoric times in order to give taste and aroma in food production. In recent years, the treatment method with plants of great interest especially in abroad, has started to gain importance in our country. The present study was planed to determine antioxidant and antimicrobial properties of Prangos pabularia which growed naturally in Bitlis city. The samples of P. pabularia plant were picked up from Kambos Mountain in Bitlis. Essential oils of leaves and fruits of plant obtained by water distillation. To determine antioxidant properties, DPPH method was used. Antioxidant activities of fruits and leaves of the plant were determined as 28.70% and 25.54%, IC₅₀ values detected as 15.43 µg/ml and 16.73 µg/ml respectively. Antimicrobial activity was detected by disc diffusion method. The essential oil of fruits of plant showed thin antibiotic activity against Proteus mirabilis and Enterobacter aurogenes. The essential oil of leaves of the plant did not show antibiotic effect against any bacterial or fungi strain.

Keywords

• Prangos pabularia,Apiaceae,Antioxidant,Antimicrobial

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