Araştırma Makalesi
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Probiyotiklerin Kültürde Mezenkimal Kök Hücre Davranışına Etkisi

Yıl 2022, , 7 - 16, 28.02.2022
https://doi.org/10.33716/bmedj.1038292

Öz

Amaç: Probiyotik (PB) ve Mezenkimal Kök Hücre (MKH) yeni tedavi fikirleridir. PB ile değiştirilen mikrobiyata farklı etkiler yapmaktadır. Son zamanlarda önemli araştırma konusu olmuştur. MKH hücresel tedavi kapsamında MKH Graft Versus Host (GVH) gibi hastalıklarda klinik uygulamada yerini almaktadır. Bu çalışmada kültür ortamında MKH davranışına çoğalma, göç etme, farklanma ve yara iyileşmesi açılarından PB uygulamasının yapacağı etkiler araştırıldı.Gereç ve Yöntem: Dondurulmuş yağ dokusu kökenli MKH (YDMKH) hücre kültüre alınarak oniki kuyucuklu kültür tabağında 2.5x104hücre/cm2 olacak şekilde üretildi. Çoğalması MTT ile, göç etmesi çizik oluşturulması sonrası yara yerinin kapanması ile, farklanması kemik hücresi osteoblasta dönüştürülmesi ile incelendi. PB olarak NBL Probiotic Gold. Preparatı içersindeki 2.5x109 Enterococcus faecium, 2.5x109 lactobacillus acidophilus, 2.5x109lactobacillus rhamnosus, 2.5x109Bifidobacterium longum, 2.5x109Bifidobacterium bifidum bakterileri eklenip aynı ortamdaki MKH için davranış parametreleri açısından incelendi. PB ve MKH ilişkisi taramalı elektron mikroskobi ile de araştırıldı. Bulgular: PB eklenmesinin çoğalmayı hızlandırdığı, göç etmeyi arttırarak yara iyileşmesini çabuklaştırdığı ve osteoblastik kemiksi yapıya farklanmada olumlu katkısının oluştuğu saptandı. İnce yapı incelemesinde PB ile etkileşmiş MKH için daha canlı, daha çok tutunan ve daha aktif hücre morfolojisinde olduğu bulundu. Sonuç: Alınan sonuçlar kültür ortamına PB eklenmesinin MKH etkinliğini arttırdığını gösterdi. Deney hayvanı ile yapılacak çalışmalarda aynı olumlu etkinin görülmesinin klinik için yararlı olacağı düşünüldü.

Destekleyen Kurum

İZMİR DEMOKRASİ ÜNİVERSİTESİ BAP KOMİSYONU

Proje Numarası

TPF-20H05

Teşekkür

Bu Çalışmayı Destekleyen BAP Komisyonuna ve Üniversitemize teşekkürederim

Kaynakça

  • Tan Y, Wei Z, Chen J, An J, Li M, Zhou L, Men Y, Zhao S. Save your gut save your age: The role of the microbiome in stem cell ageing. J Cell Mol Med. 2019 Jun 17.
  • Qin Y, Wade PA. Crosstalk between the microbiome and epigenome: messages from bugs. J Biochem. 2018 Feb 1;163(2):105- 112.
  • Xiao E, He L, Wu Q, Li J, He Y, Zhao L, Chen S et al.Microbiota regulates bone marrow mesenchymal stem cell lineage differentiation and immunomodulation. Stem Cell Res Ther. 2017 Sep 29;8(1):213.
  • Andermann TM, Rezvani A, Bhatt AS. Microbiota Manipulation With Prebiotics and Probiotics in Patients Undergoing Stem Cell Transplantation. Curr Hematol Malig Rep. 2016 Feb;11(1):19-28. doi: 10.1007/s11899-016-0302-9. PMID: 26780719;
  • Peck BCE, Shanahan MT, Singh AP, Sethupathy P. Gut Microbial Influences on the Mammalian Intestinal Stem Cell Niche. Stem Cells Int. 2017; 2017:5604727
  • Hou Q, Ye L, Huang L, Yu Q. The Research Progress on Intestinal Stem Cells and Its Relationship with Intestinal Microbiota.Front Immunol. 2017 May 23; 8:599.
  • Karaöz, E., Aksoy, A., Ayhan, S., Sariboyaci, AE., Kaymaz, F., Kasap, M.). Characterization Of Mesenchymal Stem Cells From Rat Bone Marrow: Ultrastructural Properties, Differentiation Potential And İmmunophenotypic Markers, Histochemistry and Cell Biology, 2009; 132: 533-46.
  • Michael S, Achilleos C, Panayiotou T, Strati K. Inflammation Shapes Stem Cells and Stemness during Infection and Beyond.Front Cell Dev Biol. 2016; 4:118.
  • Sureda, A., Bader, P., Cesaro, S. et al. Indications for allo- and auto-SCT for haematological diseases, solid tumours and immune disorders: current practice in Europe, 2015. Bone Marrow Transplant 50, 1037–1056 (2015). https://doi.org/10.1038/ bmt.2015.6
  • Liu H, Gu R, Li W, Zhou W, Cong Z, Xue J, Liu Yet al. Lactobacillus rhamnosus GG attenuates tenofovir disoproxil fumarateinduced bone loss in male mice via gut-microbiota-dependent anti-inflammation. Ther Adv Chronic Dis. 2019 Jul 3;10
  • Jensen GS, Cash HA, Farmer S, Keller D. Inactivated probiotic Bacillus coagulans GBI-30 induces complex immune activating, anti-inflammatory, and regenerative markers in vitro. J Inflamm Res. 2017 Aug 7;10: 107-117.
  • Pierzchalska M, Panek M, Czyrnek M, Grabacka M. Correction to: The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine. Methods Mol Biol. 2019; 1576:355.
  • Karakayali M, Önal T, Tuglu Mİ. Effect of mesenchymal stem cells and their niche on diabetic and osteoporotic wound healing following osteogenic differentiation and bone matrix formation in vitro. 2019, Cilt 13, Sayı 3, 183- 192
  • Karakayali M, Önal T, Tuglu Mİ. Investigation of the moderate toxicity of agricultural pesticides cyantraniliprole, boscalid and spiromesifen in vitro using neurotoxicity screening test, 2021, Cilt 15, Sayı 1, 1 – 10.
  • Mete M, Aydemir I, Ünlü Ünsal Ü, Duransoy YK, Tuğlu İM, Selçuki M. Neuroprotective effects of bone marrow-derived mesenchymal stem cells and conditioned medium in mechanically injured neuroblastoma cells. Turk J Med Sci. 2016 Dec 20;46(6):1900-1907. doi: 10.3906/sag-1507-187.
  • Han N, Jia L, Guo L, Su Y, Luo Z, Du J, Mei S et al. Balanced oral pathogenic bacteria and probiotics promoted wound healing via maintaining mesenchymal stem cell homeostasis. Stem Cell Res Ther. 2020 Feb 14;11(1):61. doi: 10.1186/s13287-020- 1569-2.
  • Atesci AA, Avci CB, Tuglu MI, Ozates Ay NP, Eronat AC. Effect of Different Dentin Conditioning Agents on Growth Factor Release, Mesenchymal Stem Cell Attachment and Morphology. J Endod. 2019 Dec 4. pii: S0099-2399(19)30843-X. doi: 10.1016/j.joen.2019.10.033.
  • Wu ZY, Sun Q, Liu M, Grottkau BE, He ZX, Zou Q, Ye C. Correlation between the efficacy of stem cell therapy for osteonecrosis of the femoral head and cell viability. BMC Musculoskelet Disord. 2020 Jan 29;21(1):55.
  • Yeom J, Ma S, Lim YH. Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio. Microorganisms. 2021 Mar 24;9(4):673. doi: 10.3390/microorganisms9040673. PMID: 5.Oliveria PD
  • Chen C, Dong B, Wang Y, Zhang Q, Wang B, Feng S, Zhu Y. The role of Bacillus acidophilus in osteoporosis and its roles in proliferation and differentiation. J Clin Lab Anal. 2020 Nov;34(11): e23471. doi: 10.1002/jcla.23471. Epub 2020 Aug 11.
  • Fariba Sadraei ,Marzieh Ghollasi ,Karaj Fatemeh Khakpai https://orcid.org/0000-0002-3363-8276 Osteogenic Differentiation of Pre-Conditioned Bone Marrow Mesenchymal Stem Cells with Nisin on the Modified Poly-L-Lactic-Acid Nanofibers Research Square, 2021.

Effect of probiotics on mesenchymal stem cell behavior in culture

Yıl 2022, , 7 - 16, 28.02.2022
https://doi.org/10.33716/bmedj.1038292

Öz

Aim: Probiotic (PB) and Mesenchymal Stem Cell (MSC) are new treatment ideas for diseases. Alterations of microbiata made by PB produce different effects. MSC as the scope of cellular therapy takes place in clinical practice in many diseases such as Graft Versus Host (GVD) within the scope of cellular therapy. In this study, the effects of PB application on MSC behavior in culture in terms of proliferation, migration, differentiation and wound healing were investigated. Material and Method: Frozen adipose tissue-derived (AD) MSC cells were cultured and produced at 2.5x104 cells/cm2 in a twelve-well culture dish. They were examined for proliferation by MTT, migration by the closure of the wound after the creation of a scratch, and differentiation by transforming the bone cell osteoblast. NBL Probiotic Gold as PB was used as bacteria which include 2.5x109 Enterococcus faecium, 2.5x109lactobacillus acidophilus, 2.5x109lactobacillus rhamnosus, 2.5x109Bifidobacterium longum, 2.5x109Bifidobacterium bifidum and examined in terms of behavioral parameters for MSC in the same environment. The relationship between PB and MSC was also investigated by Scanning Electron Microscopy (SEM). Result: It was determined that use of PB caused an increase in proliferation, faster wound closure by increase of migration and higher differentiation to osteoblast. The ultrastructural examination of MSC by SEM showed that MSCs interacted with PB are more spread, more adherent, and have more active cell morphology. Conclusion: The results showed that the addition of PB to the culture medium increased the MSC efficiency. It was thought that PB would be more beneficial for the clinic to see of MSC if the same positive effect results in studies with experimental animals.

Proje Numarası

TPF-20H05

Kaynakça

  • Tan Y, Wei Z, Chen J, An J, Li M, Zhou L, Men Y, Zhao S. Save your gut save your age: The role of the microbiome in stem cell ageing. J Cell Mol Med. 2019 Jun 17.
  • Qin Y, Wade PA. Crosstalk between the microbiome and epigenome: messages from bugs. J Biochem. 2018 Feb 1;163(2):105- 112.
  • Xiao E, He L, Wu Q, Li J, He Y, Zhao L, Chen S et al.Microbiota regulates bone marrow mesenchymal stem cell lineage differentiation and immunomodulation. Stem Cell Res Ther. 2017 Sep 29;8(1):213.
  • Andermann TM, Rezvani A, Bhatt AS. Microbiota Manipulation With Prebiotics and Probiotics in Patients Undergoing Stem Cell Transplantation. Curr Hematol Malig Rep. 2016 Feb;11(1):19-28. doi: 10.1007/s11899-016-0302-9. PMID: 26780719;
  • Peck BCE, Shanahan MT, Singh AP, Sethupathy P. Gut Microbial Influences on the Mammalian Intestinal Stem Cell Niche. Stem Cells Int. 2017; 2017:5604727
  • Hou Q, Ye L, Huang L, Yu Q. The Research Progress on Intestinal Stem Cells and Its Relationship with Intestinal Microbiota.Front Immunol. 2017 May 23; 8:599.
  • Karaöz, E., Aksoy, A., Ayhan, S., Sariboyaci, AE., Kaymaz, F., Kasap, M.). Characterization Of Mesenchymal Stem Cells From Rat Bone Marrow: Ultrastructural Properties, Differentiation Potential And İmmunophenotypic Markers, Histochemistry and Cell Biology, 2009; 132: 533-46.
  • Michael S, Achilleos C, Panayiotou T, Strati K. Inflammation Shapes Stem Cells and Stemness during Infection and Beyond.Front Cell Dev Biol. 2016; 4:118.
  • Sureda, A., Bader, P., Cesaro, S. et al. Indications for allo- and auto-SCT for haematological diseases, solid tumours and immune disorders: current practice in Europe, 2015. Bone Marrow Transplant 50, 1037–1056 (2015). https://doi.org/10.1038/ bmt.2015.6
  • Liu H, Gu R, Li W, Zhou W, Cong Z, Xue J, Liu Yet al. Lactobacillus rhamnosus GG attenuates tenofovir disoproxil fumarateinduced bone loss in male mice via gut-microbiota-dependent anti-inflammation. Ther Adv Chronic Dis. 2019 Jul 3;10
  • Jensen GS, Cash HA, Farmer S, Keller D. Inactivated probiotic Bacillus coagulans GBI-30 induces complex immune activating, anti-inflammatory, and regenerative markers in vitro. J Inflamm Res. 2017 Aug 7;10: 107-117.
  • Pierzchalska M, Panek M, Czyrnek M, Grabacka M. Correction to: The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine. Methods Mol Biol. 2019; 1576:355.
  • Karakayali M, Önal T, Tuglu Mİ. Effect of mesenchymal stem cells and their niche on diabetic and osteoporotic wound healing following osteogenic differentiation and bone matrix formation in vitro. 2019, Cilt 13, Sayı 3, 183- 192
  • Karakayali M, Önal T, Tuglu Mİ. Investigation of the moderate toxicity of agricultural pesticides cyantraniliprole, boscalid and spiromesifen in vitro using neurotoxicity screening test, 2021, Cilt 15, Sayı 1, 1 – 10.
  • Mete M, Aydemir I, Ünlü Ünsal Ü, Duransoy YK, Tuğlu İM, Selçuki M. Neuroprotective effects of bone marrow-derived mesenchymal stem cells and conditioned medium in mechanically injured neuroblastoma cells. Turk J Med Sci. 2016 Dec 20;46(6):1900-1907. doi: 10.3906/sag-1507-187.
  • Han N, Jia L, Guo L, Su Y, Luo Z, Du J, Mei S et al. Balanced oral pathogenic bacteria and probiotics promoted wound healing via maintaining mesenchymal stem cell homeostasis. Stem Cell Res Ther. 2020 Feb 14;11(1):61. doi: 10.1186/s13287-020- 1569-2.
  • Atesci AA, Avci CB, Tuglu MI, Ozates Ay NP, Eronat AC. Effect of Different Dentin Conditioning Agents on Growth Factor Release, Mesenchymal Stem Cell Attachment and Morphology. J Endod. 2019 Dec 4. pii: S0099-2399(19)30843-X. doi: 10.1016/j.joen.2019.10.033.
  • Wu ZY, Sun Q, Liu M, Grottkau BE, He ZX, Zou Q, Ye C. Correlation between the efficacy of stem cell therapy for osteonecrosis of the femoral head and cell viability. BMC Musculoskelet Disord. 2020 Jan 29;21(1):55.
  • Yeom J, Ma S, Lim YH. Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio. Microorganisms. 2021 Mar 24;9(4):673. doi: 10.3390/microorganisms9040673. PMID: 5.Oliveria PD
  • Chen C, Dong B, Wang Y, Zhang Q, Wang B, Feng S, Zhu Y. The role of Bacillus acidophilus in osteoporosis and its roles in proliferation and differentiation. J Clin Lab Anal. 2020 Nov;34(11): e23471. doi: 10.1002/jcla.23471. Epub 2020 Aug 11.
  • Fariba Sadraei ,Marzieh Ghollasi ,Karaj Fatemeh Khakpai https://orcid.org/0000-0002-3363-8276 Osteogenic Differentiation of Pre-Conditioned Bone Marrow Mesenchymal Stem Cells with Nisin on the Modified Poly-L-Lactic-Acid Nanofibers Research Square, 2021.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Klinik Tıp Bilimleri
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Emine Müge Karakayalı 0000-0001-5779-4102

Tuna Önal

Zeynep Gülden Sönmez Tamer Bu kişi benim 0000-0003-4342-2652

Mehmet İbrahim Tuğlu 0000-0002-0569-8415

Proje Numarası TPF-20H05
Yayımlanma Tarihi 28 Şubat 2022
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Karakayalı, E. M., Önal, T., Sönmez Tamer, Z. G., Tuğlu, M. İ. (2022). Probiyotiklerin Kültürde Mezenkimal Kök Hücre Davranışına Etkisi. Balıkesir Medical Journal, 6(1), 7-16. https://doi.org/10.33716/bmedj.1038292