Boron determination in serum and plasma of rats administered boron citrate

Öz

Boron is a dynamic trace element that is very important for animal and human biology. Boron citrate is one of the most common forms of boron uptake into metabolism. In this study, the amount of boron was determined by ICP-MS method in both plasma and serum of rats administered boron citrate orally. Boron concentrations in serum and plasma samples taken at different times from rats administered orally 20 mg/kg boron citrate were determined and the graph of change over time was examined. It was observed that serum and plasma boron levels reached the maximum in 0.25 hours after boron citrate was applied, and boron level decreased to normal plasma and serum levels after 48 hours. We think that this study will form the basis for boron analysis and pharmacokinetic properties of boron.

Anahtar Kelimeler

• Boron citrate
• ICP-MS
• Plasma
• Rat
• Serum

Bor sitrat uygulanan ratların serum ve plazmalarında bor tayini

Öz

Bor, hayvan ve insan biyolojisi için çok önemli olan dinamik bir eser elementtir. Bor sitrat, borun metabolizmaya alımındaki en yaygın formlardan biridir. Bu çalışmada oral olarak bor sitrat uygulanan ratların hem plazmalarında hem de serumlarında ICP-MS yöntemi ile bor miktarı tayin edilmiş ve oral olarak 20 mg/kg bor sitrat uygulanan ratlardan farklı zamanlarda alınan serum ve plazma numunelerindeki bor konsantrasyonları tayin edilerek zamana göre değişim grafiği incelenmiştir. Bor sitrat ugulandıkdan sonra 0,25. saatte serum ve plazma bor seviyesinin maksimuma ulaştığı, 48. saatten sonra bor seviyesinin normal plazma ve serum seviyesine indiği görülmüştür. Bu çalışmanın bor analizleri ve borun farmakokinetik özellikleri açısından temel teşkil edeceğini düşünmekteyiz.

Anahtar Kelimeler

• bor sitrat
• ICP-MS
• Plazma
• Rat
• Serum

Kaynakça

1. 1. Khaliq, H., Juming, Z. & Ke-Mei, P. (2018) The physiological role of boron on health. Biological trace element research, 186(1): p. 31-51.
2. 2. Kot, F.S. (2009) Boron sources, speciation and its potential impact on health. Reviews in Environmental Science and Bio/Technology, 8(1): p. 3-28.
3. 3. Briggs, M. (2000). Boron Oxides, Boric Acid, and Borates. Kirk-Othmer Encyclopedia of Chemical Technology, 4, 241-294.
4. 4. Mogoşanu, G. D., Biţă, A., Bejenaru, L. E., Bejenaru, C., Croitoru, O., Rău, G., ... & Scorei, R. I. (2016). Calcium fructoborate for bone and cardiovascular health. Biological trace element research, 172(2), 277-281.
5. 5. Armstrong, T. A., Spears, J. W., Crenshaw, T. D., & Nielsen, F. H. (2000). Boron supplementation of a semipurified diet for weanling pigs improves feed efficiency and bone strength characteristics and alters plasma lipid metabolites. The Journal of nutrition, 130(10), 2575-2581.
6. 6. Pizzorno, L. (2015). Nothing boring about boron. Integrative Medicine: A Clinician's Journal, 14(4), 35.
7. 7. Toker, H., Ozdemir, H., Yuce, H. B., & Goze, F. (2016). The effect of boron on alveolar bone loss in osteoporotic rats. Journal of Dental Sciences, 11(3), 331-337.
8. 8. Hakki, S. S., Malkoc, S., Dundar, N., Kayis, S. A., Hakki, E. E., Hamurcu, M., ... & Götz, W. (2015). Dietary boron does not affect tooth strength, micro-hardness, and density, but affects tooth mineral composition and alveolar bone mineral density in rabbits fed a high-energy diet. Journal of Trace Elements in Medicine and Biology, 29, 208-215.

9. 9. Hakki, S. S., Bozkurt, B. S., & Hakki, E. E. (2010). Boron regulates mineralized tissue-associated proteins in osteoblasts (MC3T3-E1). Journal of Trace Elements in Medicine and Biology, 24(4), 243-250. 10. Coban, F. K., Ince, S., Kucukkurt, I., Demirel, H. H., & Hazman, O. (2015). Boron attenuates malathion-induced oxidative stress and acetylcholinesterase inhibition in rats. Drug and chemical Toxicology, 38(4), 391-399.
10. 11. Kuru, R., & Yarat, A. (2017). Bor ve Sagligimiza Olan Etkilerine Guncel Bir Bakis [Boron and a Current Overview of its Effects on Health]. Clinical and Experimental Health Sciences, 7(3), 107-115.
11. 12. Demirci, S., Doğan, A., Karakuş, E., Halıcı, Z., Topçu, A., Demirci, E., & Sahin, F. (2015). Boron and poloxamer (F68 and F127) containing hydrogel formulation for burn wound healing. Biological trace element research, 168(1), 169-180.
12. 13. Nielsen, F. H. (2014). Update on human health effects of boron. Journal of Trace Elements in Medicine and Biology, 28(4), 383-387.
13. 14. Penland, J. G. (1994). Dietary boron, brain function, and cognitive performance. Environmental health perspectives, 102(suppl 7), 65-72.
14. 15. Nielsen, F. H., Mullen, L. M., & Nielsen, E. J. (1991). Dietary boron affects blood cell counts and hemoglobin concentrations in humans. ISSN: 1520-670X
15. 16. Aysan, E., Sahin, F., Telci, D., Erdem, M., Muslumanoglu, M., Yardımcı, E., & Bektasoglu, H. (2013). Mechanism of body weight reducing effect of oral boric acid intake. International Journal of Endocrinology, 2013.
16. 17. Murray, F. J. (1998). A comparative review of the pharmacokinetics of boric acid in rodents and humans. Biological trace element research, 66(1), 331-341.
17. 18. Kelly, G. S. (1997). Boron: a review of its nutritional interactions and therapeutic uses. Altern Med Rev, 2(1), 48-56.
18. 19. Volpe, S. L., Taper, L. J., & Meacham, S. (1993). The relationship between boron and magnesium status and bone mineral density in the human: a review. Magnesium Research, 6(3), 291-296.
19. 20. Ghanizadeh, G., Babaei, M., Naghii, M. R., Mofid, M., Torkaman, G., & Hedayati, M. (2014). The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat. Toxicology and industrial health, 30(3), 211-217.
20. 21. Tsygankova, A. R., Kanygin, V. V., Kasatova, A. I., Zav’yalov, E. L., Gusel’nikova, T. Y., Kichigin, A. I., & Mukhamadiyarov, R. A. (2020). Determination of boron by inductively coupled plasma atomic emission spectroscopy. Biodistribution of 10B in tumor-bearing mice. Russian Chemical Bulletin, 69(3), 601-607.
21. 22. Green, N. R., & Ferrando, A. A. (1994). Plasma boron and the effects of boron supplementation in males. Environmental Health Perspectives, 102(suppl 7), 73-77.
22. 23. Ferrando, A. A., Green, N. R., Barnes, K. W., & Woodward, B. (1993). Microwave digestion preparation and ICP determination of boron in human plasma. Biological trace element research, 37(1), 17-25.
23. 24. Atila, A., Halici, Z., Cadirci, E., Karakus, E., Palabiyik, S. S., Ay, N., ... & Yilmaz, S. (2016). Study of the boron levels in serum after implantation of different ratios nano-hexagonal boron nitride–hydroxy apatite in rat femurs. Materials Science and Engineering: C, 58, 1082-1089.
24. 25. Mahmood, N. M. A., Barawi, O. R., & Hussain, S. A. (2016). Relationship between serum concentrations of boron and inflammatory markers, disease duration, and severity of patients with knee osteoarthritis in Sulaimani city. National Journal of Physiology, Pharmacy and Pharmacology, 6(1), 27-31.
25. 26. Korkmaz, M., Şaylı, U., Şaylı, B. S., Bakırdere, S., Titretir, S., Ataman, O. Y., & Keskin, S. (2007). Estimation of human daily boron exposure in a boron-rich area. British journal of nutrition, 98(3), 571-575.
26. 27. Sun, D. H., Ma, R. L., McLeod, C. W., Wang, X. R., & Cox, A. G. (2000). Determination of boron in serum, plasma and urine by inductively coupled plasma mass spectrometry (ICP-MS). Use of mannitol-ammonia as diluent and for eliminating memory effect. Journal of Analytical Atomic Spectrometry, 15(3), 257-261.
27. 28. Nielsen, F. H., & Meacham, S. L. (2011). Growing evidence for human health benefits of boron. Journal of Evidence-Based Complementary & Alternative Medicine, 16(3), 169-180.
28. 29. Demircan, B., & Velioğlu, Y. S. (2020). Gıda ve cevreden alınan Bor bileşiklerinin toksikolojik değerlendirmesi. Akademik Gıda, 18(3), 312-322.
29. 30. Usuda, K., Kono, K., Orita, Y., Dote, T., Iguchi, K., Nishiura, H., ... & Shirai, Y. (1998). Serum and urinary boron levels in rats after single administration of sodium tetraborate. Archives of toxicology, 72(8), 468-474.
30. 31. Lee, I. P., Sherins, R. J., & Dixon, R. L. (1978). Evidence for induction of germinal aplasia in male rats by environmental exposure to boron. Toxicology and applied pharmacology, 45(2), 577-590.
31. 32. Vaziri, N. D., Oveisi, F., Culver, B. D., Pahl, M. V., Andersen, M. E., Strong, P. L., & Murray, F. J. (2001). The effect of pregnancy on renal clearance of boron in rats given boric acid orally. Toxicological Sciences, 60(2), 257-263.
32. 33. Nielsen, F. H., & Penland, J. G. (1999). Boron supplementation of peri‐menopausal women affects boron metabolism and indices associated with macromineral metabolism, hormonal status and immune function. The Journal of Trace Elements in Experimental Medicine: The Official Publication of the International Society for Trace Element Research in Humans, 12(3), 251-261.