Boronik asit moleküllerinin Pseudomonas aeruginosa’da virülens faktörlerinin üretimine etkisi

Öz

Hücreler arası iletişim sistemi (quorum sensing system (QS)), bakterilerin birbirleriyle oto-indükleyici olarak adlandırılan, küçük difüze olabilen moleküller kullanarak iletişim kurduğu bir sistemdir. Bu sistemin bakterilerde, virülans faktörlerinin üretimi de dahil olmak üzere pek çok fonksiyonu kontrol ettiği, yapılan çalışmalarla gösterilmiştir. Bu çalışmanın amacı, Pseudomonas aeruginosa’da üretimi hücreler arası iletişim sistemi ile kontrol edilen virülens faktörleri üretimine boronik asit moleküllerinin potansiyel inhibitör etkisinin araştırılmasıdır. Bu çalışmayla bazı boronik asit türevi moleküllerin P. aeruginosa PA01 suşunda virülens faktörlerinin (elastaz ve piyosiyanin) üretimi, biyofilm oluşumu ve kayma hareketi üzerine etkileri araştırılmıştır. Etkisi incelenen boronik asit moleküllerinden 4-Bromofenilboronik asitin, elastaz ve piyosiyanin üretimi, biyofilm oluşumu ve kayma hareketini istatiksel olarak önemli derecede azalttığı belirlenmiştir. Bu çalışma ile boronik asit moleküllerinin P. aeruginosa gibi patojenlerin virülens faktörlerinin üretimini engelleyebildiği belirlenmiştir.

Anahtar Kelimeler

• Pseudomonas aeruginosa,Boronik asit,Hücrelerarası iletişim sistemi

Effects of boronic acids on the production of virulence factors by Pseudomonas aeruginosa

Öz

The intercellular communication (Quorum sensing) in bacteria is based on using small diffusible signal molecules called as autoinducers to communicate with each other. Studies have shown that QS controls a large number of microbial processes, including

the expression of virulence factors. The aim of this work is to investigate the potential inhibitory effect of boronic acid molecules on the production of virulence factors controlled by the intercellular communication system in Pseudomonas aeruginosa. In this study, the effects of some boronic acid derivatives were investigated on the production of virulence factors (elastase and pyocyanin), biofilm formation and swarming motility in P. aeruginosa PA01. The results indicate that production of elastase and pyocyanin, the formation of biofilm and swarming motility were significantly reduced by 4-Bromofenilboronic acid. This study tends to support the potential inhibitory effects of boronic acid derivatives on the production of virulence factors in some pathogens such as P. aeruginosa.

Anahtar Kelimeler

• Pseudomonas aeruginosa,Boronic acid,Quorum sensing system

Kaynakça

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