Farklı Çözücülerle Hazırlanan Elettaria Cardamomum (L.) Maton Ekstraktlarının Antibakteriyel Aktivitelerinin İncelenmesi

Yıl 2024, , 635 - 640, 15.07.2024

Hatice Taner Saraçoğlu

https://doi.org/10.34248/bsengineering.1473309

Öz

Bu çalışmanın amacı Elettaria cardamomum’un tohumlarından hazırlanan ekstraktların çeşitli bakterilere karşı olan antibakteriyel aktivitelerinin belirlenmesidir. Bu çalışmada, Elettaria cardamomum bitkisinin tohumlarından farklı çözücüler (etanol, aseton, etil asetat, kloroform, soğuk su, sıcak su) kullanılarak ekstraktları hazırlanmıştır. Ekstraktların beş patojen bakteri suşuna (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 29853) karşı olan antibakteriyel aktiviteleri disk difüzyon yöntemi kullanılarak belirlenmiştir. Çalışma sonuçları etanol, aseton, etil asetat ve kloroform ekstraktlarının Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633 ve Klebsiella pneumoniae ATCC 700603 bakterilerine karşı farklı derecelerde antibakteriyel aktiviteye sahip olduğunu göstermiştir. Çalışmadaki tüm ekstraktlar Escherichia coli ATCC 25922 ve Pseudomonas aeruginosa ATCC 29853 bakterilerine karşı antibakteriyel aktivite göstermemiştir. En yüksek antibakteriyel aktiviteyi etil asetat ekstraktı Staphylococcus aureus ATCC 29213 bakterisine karşı göstermiştir.

Anahtar Kelimeler

Antibakteriyel aktivite, Elettaria cardamomum, Farklı çözücüler, Ekstrakt

Investigation of Antibacterial Activities of Elettaria Cardamomum (L.) Maton Extracts Prepared with Different Solvents

Öz

The aim of this study is to determine the antibacterial activities of extracts prepared from the seeds of Elettaria cardamomum against various bacteria. In this study, extracts were prepared from the seeds of the Elettaria cardamomum plant using different solvents (ethanol, acetone, ethyl acetate, chloroform, cold water, hot water). The antibacterial activities of the extracts against five pathogenic bacterial strains (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 29853) were determined using the disc diffusion method. Study results showed that ethanol, acetone, ethyl acetate and chloroform extracts had different degrees of antibacterial activity against Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633 and Klebsiella pneumoniae ATCC 700603 bacteria. All extracts in the study did not show antibacterial activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 29853 bacteria. Ethyl acetate extract showed the highest antibacterial activity against Staphylococcus aureus ATCC 29213 bacteria.

Anahtar Kelimeler

Antibacterial activity, Elettaria cardamomum, Different solvents, Extract

Kaynakça

• Ağaoğlu S, Dostbil N, Alemdar S. 2005. Antimicrobial effect of seed extract of Cardamom (Elettaria cardamomum Maton). YYÜ Vet Fak Derg, 16(2): 99-101.
• Ahmad I, Mahmood Z, Mohammad F. 1998. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol, 62: 183-193.
• Al-Zuhair H, El-Sayeh B, Ameen HA, Al-Shoora H. 1996. Pharmacological studies of cardamom oil in animals. Pharmacol Res, 34(1-2): 79-82.
• Arora DS, Kaur GJ. 2007. Antibacterial activity of some Indian medicinal plants. J Nat Med, 61: 313-317.
• Bisht VK, Negi JS, Bhandari AK, Sundriyal RC. 2011. Amomum subulatum Roxb: Traditional, phytochemical and biological activities-An overview. Afr J Agric Res, 6(24): 5386-5390.
• Bradshaw LJ. 1992. Laboratory of Microbiology. Saunders College Publishing, USA. 4th ed., pp: 435.
• Ceylan E, Fung DYC. 2004. Antimicrobial activity of spices. J Rapid Meth Aut Mic, 12(1): 1-55.
• Collins CM, Lyne PM, Grange JM. 1989. Microbiological Methods. Butterworths, London, 6th ed., pp: 410.
• El Malti J, Mountassif D, Amarouch H. 2007. Antimicrobial activity of Elettaria cardamomum: Toxicity, biochemical and histological studies. Food Chem, 104(4): 1560-1568.
• Hamzaa RG, Osman NN. 2012. Using of coffee and cardamom mixture to ameliorate oxidative stress induced in γ-irradiated rats. Biochem Anal Biochem, 1(113): 2161-1009.
• Jiang TA. 2019. Health benefits of culinary herbs and spices. J AOAC Int, 102: 395-411.
• Kapoor LD. 2000. Handbook of Ayurvedic Medicinal Plants. CRC press, Boca Raton, FL, pp: 424.
• Kaushik P, Goyal P, Chauhan A, Chauhan G. 2010. In vitro evaluation of antibacterial potential of dry fruit extracts of Elettaria cardamomum Maton (Chhoti Elaichi). IJPR, 9(3): 287-292.
• Madsen HL, Bertelsen G. 1995. Spices as antioxidants. Trends Food Sci Technol, 6: 271-277.
• Ravindran PN. 2002. Introduction. In: Ravindran PN, Madhusoodanan KJ, editors, Cardamom-the genus Elettaria. CRC Press, London, 1st ed., pp: 1-10.
• Sengottuvelu S. 2011. Cardamom (Elettaria cardamomum Linn. Maton) seeds in health. In: Preedy VR, Watson RS, Patel VB, editors. Nuts and seeds in health and disease prevention. Elsevier, Amsterdam, 1st ed., pp: 285-291.
• Singh G, Kapoor IPS, Singh P, De Heluani CS, De Lampasona MP, Catalan CAN. 2008. Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food Chem Toxicol, 46: 3295-3302.
• Wojdyło A, Oszmiański J, Czemerys R. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem, 105: 940-949.
• Yassin MT, Mostafa AAF, Al-Askar AA. 2020. In vitro anticandidal potency of Syzygium aromaticum (clove) extracts against vaginal candidiasis. BMC Complement Med Ther, 20(1): 1-9.
• Yassin MT, Mostafa AAF, Al-Askar AA, Alkhelaif AS. 2022. In vitro antimicrobial potency of Elettaria cardamomum ethanolic extract against multidrug resistant of food poisoning bacterial strains. J King Saud Univ Sci, 34(6): 102167.