Research on Aptamer Integrated Surface-Enhanced Raman Spectroscopy Platforms for Diagnosis: Bibliometric Analysis of Topics and Trends

Öz

Aptamer integrated SERS platforms have become a remarkable and effective method in the diagnosis of various diseases, including cancer. In addition to the advantages of SERS, such as its unique molecular fingerprint identification and ease of use, aptamers offer multiple detection capabilities and more accurate measurements in complex environments. In this study, a bibliometric analysis was conducted on research related to aptamer-integrated SERS platforms for disease diagnosis in order to evaluate developments in the field. Considering all exclusion criteria, a total of 33 publications were obtained with 198 authors contributing and articles published in 24 journals. The average number of citations per document was 16.67, while the international collaboration rate was calculated as 15.15%. China, as the most productive and most cited country, seems to have a significant impact in this field. Furthermore, Cao Xiaowei, one of the most published authors, is Chinese and has the highest h-index among the authors. The most cited article was also produced in collaboration with China. In conclusion, it can be said that China haa a significant influence in this field. Keyword analysis also shows increasing interest in studies on the use of aptamer-based SERS systems in the diagnosis of Alzheimer's disease. A bibliometric analysis of publications on aptamer integrated SERS diagnostic platforms is not currently available in the literature. This study will significantly contribute to the authors gaining different perspectives and establishing new collaboration networks, thus enabling them to develop more effective strategies for use in diagnostic applications.

Anahtar Kelimeler

• Aptamer
• Bibliometric Analysis
• Diagnosis
• Raman Spectroscopy
• Surface Enhanced Raman Spectroscopy (SERS)

Etik Beyan

Ethics committee approval was not required for this study because of there was no study on animals or humans.

Kaynakça

1. Al Abdullah, S., Ghadami, S., Khan, Md. A. R., Ebrahimi, F., Nowlin, K., Ignatova, T., & Dellinger, K. (2025). A SERS‐Based Antibody‐Aptamer Sandwich Assay for Extracellular Vesicle‐Associated Tau Detection Using Gold Nanoparticles. Advanced Sensor Research, 4(8). https://doi.org/10.1002/adsr.202500034
2. Alkhamis, O., Byrd, C., Canoura, J., Bacon, A., Hill, R., & Xiao, Y. (2025). Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity. Nucleic Acids Research, 53(6). https://doi.org/10.1093/nar/gkaf219
3. Anantha Rajah, D., Tan, H. S., & Farghadani, R. (2025). Aptamers as immune checkpoint inhibitors in cancer immunotherapy: Targeting CTLA-4/B7 and PD-1/PD-L1 pathways. International Immunopharmacology, 164, 115339. https://doi.org/10.1016/j.intimp.2025.115339
4. Aria, M., & Cuccurullo, C. (2017). bibliometrix: An R-tool for comprehensive science mapping analysis. Journal of Informetrics, 11(4), 959–975. https://doi.org/10.1016/j.joi.2017.08.007
5. Björk, B.-C. (2017). Open access to scientific articles: A review of benefits and challenges. Internal and Emergency Medicine, 12(2), 247–253. https://doi.org/10.1007/s11739-017-1603-2
6. Chen, H., Park, S.-K., Joung, Y., Kang, T., Lee, M.-K., & Choo, J. (2022). SERS-based dual-mode DNA aptasensors for rapid classification of SARS-CoV-2 and influenza A/H1N1 infection. Sensors and Actuators B: Chemical, 355, 131324. https://doi.org/10.1016/j.snb.2021.131324
7. Chisanga, M., Muhamadali, H., Ellis, D. I., & Goodacre, R. (2019). Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering. Applied Sciences, 9(6), 1163. https://doi.org/10.3390/app9061163
8. Cho, Y. S., Lee, E. J., Lee, G.-H., & Hah, S. S. (2015). Aptamer selection for fishing of palladium ion using graphene oxide-adsorbed nanoparticles. Bioorganic & Medicinal Chemistry Letters, 25(23), 5536–5539. https://doi.org/10.1016/j.bmcl.2015.10.056

9. Cialla-May, D., Bonifacio, A., Markin, A., Markina, N., Fornasaro, S., Dwivedi, A., Dib, T., Farnesi, E., Liu, C., Ghosh, A., Schmitt, M., & Popp, J. (2024). Recent advances of surface enhanced Raman spectroscopy (SERS) in optical biosensing. TrAC Trends in Analytical Chemistry, 181, 117990. https://doi.org/10.1016/j.trac.2024.117990
10. Cui, Y., Chen, Y., Zhang, Y., & Zhang, L. (2026). Advances in aptamer technology for target-based drug discovery. Journal of Pharmaceutical Analysis, 16(2), 101369. https://doi.org/10.1016/j.jpha.2025.101369
11. Dwivedi, S., Purohit, P., Misra, R., Pareek, P., Goel, A., Khattri, S., Pant, K. K., Misra, S., & Sharma, P. (2017). Diseases and Molecular Diagnostics: A Step Closer to Precision Medicine. Indian Journal of Clinical Biochemistry, 32(4), 374–398. https://doi.org/10.1007/s12291-017-0688-8
12. Ebrahimi, F., Ebrahimi, F., Kumari, A., Al Abdullah, S., & Dellinger, K. (2025a). AI‐assisted customizable sers‐based aptasensor for label‐free, rapid, and sensitive detection of tau: comparative analysis using random forest and convolutional neural networks. Analysis & Sensing, 6(1). https://doi.org/10.1002/anse.202500071
13. Ebrahimi, F., Kumari, A., Al Abdullah, S., Vivero-Escoto, J. L., & Dellinger, K. (2025b). Surface functionalization of citrate-stabilized gold nanoparticles with various disease-specific nonthiolated aptamers: rsm-based optimization for multifactorial disease biomarker detection. ACS Sensors, 10(2), 944–953. https://doi.org/10.1021/acssensors.4c02722
14. Egbe Vydaline, A. O., Rozhkov, S., Sosa, G., & Mallikaratchy, P. (2025). Aptamers as target-specific recognition elements in drug delivery. Advanced Drug Delivery Reviews, 226, 115685. https://doi.org/10.1016/j.addr.2025.115685
15. Ellington, A. D., & Szostak, J. W. (1990). In vitro selection of RNA molecules that bind specific ligands. Nature, 346(6287), 818–822. https://doi.org/10.1038/346818a0
16. García-Lillo, F., Sánchez-García, E., Marco-Lajara, B., & Seva-Larrosa, P. (2023). Renewable Energies and Sustainable Development: A Bibliometric Overview. Energies, 16(3), 1211. https://doi.org/10.3390/en16031211
17. George Kerry, R., Ukhurebor, K. E., Kumari, S., Maurya, G. K., Patra, S., Panigrahi, B., Majhi, S., Rout, J. R., Rodriguez-Torres, M. del P., Das, G., Shin, H.-S., & Patra, J. K. (2021). A comprehensive review on the applications of nano-biosensor-based approaches for non-communicable and communicable disease detection. Biomaterials Science, 9(10), 3576–3602. https://doi.org/10.1039/D0BM02164D
18. Ghazi, M. N., Husain, B., Heydaryan, K., Mehmandoust, S., Kadhim, S. A., & Eskandari, V. (2025). A Review of Aptamer-Based Surface-Enhanced Raman Scattering (SERS) Platforms for Cancer Diagnosis Applications. BioNanoScience, 15(1), 44. https://doi.org/10.1007/s12668-024-01673-w
19. Guérin, M., Vandevenne, M., Matagne, A., Aucher, W., Verdon, J., Paoli, E., Ducrotoy, J., Octave, S., Avalle, B., Maffucci, I., & Padiolleau-Lefèvre, S. (2025). Selection and characterization of DNA aptamers targeting the surface Borrelia protein CspZ with high-throughput cross-over SELEX. Communications Biology, 8(1), 632. https://doi.org/10.1038/s42003-025-08034-7
20. Huang, L., Sun, D.-W., Wu, Z., Pu, H., & Wei, Q. (2021). Reproducible, shelf-stable, and bioaffinity SERS nanotags inspired by multivariate polyphenolic chemistry for bacterial identification. Analytica Chimica Acta, 1167, 338570. https://doi.org/10.1016/j.aca.2021.338570
21. Jin, H., Liu, T., & Sun, D. (2024). Target-induced hot spot construction for sensitive and selective surface-enhanced Raman scattering detection of matrix metalloproteinase MMP-9. Microchimica Acta, 191(2), 105. https://doi.org/10.1007/s00604-024-06183-w
22. Kemeç, A., & Altınay, A. T. (2023). Sustainable Energy Research Trend: A Bibliometric Analysis Using VOSviewer, RStudio Bibliometrix, and CiteSpace Software Tools. Sustainability, 15(4), 3618. https://doi.org/10.3390/su15043618
23. Kositanont, U., Lertanantawong, B., Patarakul, K., Sripinitchai, S., Thawornkuno, C., Chaibun, T., Kreangkaiwal, C., Waiwinya, W., Promptmas, C., & Srisawat, C. (2025). Performance of electrochemical aptasensor as antigen test in clinical samples for early diagnosis of leptospirosis. Scientific Reports, 15(1), 9398. https://doi.org/10.1038/s41598-025-92685-3
24. Kovacevic, K. D., Gilbert, J. C., & Jilma, B. (2018). Pharmacokinetics, pharmacodynamics and safety of aptamers. Advanced Drug Delivery Reviews, 134, 36–50. https://doi.org/10.1016/j.addr.2018.10.008
25. Li, C., Chen, W., Zhang, Z., Qiu, F., Jiang, R., Fang, X., Zhang, S., Li, L., Liao, G., Shen, Y., Tang, H., Meng, H., Zhu, T., Luo, Z., & Tan, W. (2025). In Vivo SELEX: A Strategy for Identifying Ovarian Cancer-Specific Aptamers. ACS Applied Materials & Interfaces, 17(46), 63162–63171. https://doi.org/10.1021/acsami.5c17714
26. Li, X., Yang, Z., Waniss, M., Liu, X., Wang, X., Xu, Z., Lei, H., & Liu, J. (2023). Multiplexed SELEX for Sulfonamide Antibiotics Yielding a Group-Specific DNA Aptamer for Biosensors. Analytical Chemistry, 95(44), 16366–16373. https://doi.org/10.1021/acs.analchem.3c03787
27. Lin, B., Xiao, F., Jiang, J., Zhao, Z., & Zhou, X. (2023). Engineered aptamers for molecular imaging. Chemical Science, 14(48), 14039–14061. https://doi.org/10.1039/D3SC03989G
28. Ling, C. C. Y., & Fullwood, M. J. (2024). AptaFluorescence: An aptamer-based fluorescent imaging protocol for biomolecule visualization. PLOS ONE, 19(12), e0316359. https://doi.org/10.1371/journal.pone.0316359
29. Liu, Q., Jin, X., Cheng, J., Zhou, H., Zhang, Y., & Dai, Y. (2023). Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review). Molecular Medicine Reports, 27(5), 104. https://doi.org/10.3892/mmr.2023.12991
30. Liu, S., Huo, Y., Bai, J., Ning, B., Peng, Y., Li, S., Han, D., Kang, W., & Gao, Z. (2020). Rapid and sensitive detection of prostate-specific antigen via label-free frequency shift Raman of sensing graphene. Biosensors and Bioelectronics, 158, 112184. https://doi.org/10.1016/j.bios.2020.112184
31. Lv, J., Chang, S., Chen, H.-Y., Zhou, X.-Y., Wang, X.-Y., Chen, Z.-C., Chen, B.-B., Qian, R.-C., & Li, D.-W. (2023). A multi-channel responsive AuNP@COF core-shell nanoprobe for simultaneous subcellular profiling of multiple cancer biomarkers. Biosensors and Bioelectronics, 234, 115325. https://doi.org/10.1016/j.bios.2023.115325
32. Molefe, P. F., Masamba, P., Oyinloye, B. E., Mbatha, L. S., Meyer, M., & Kappo, A. P. (2018). Molecular Application of Aptamers in the Diagnosis and Treatment of Cancer and Communicable Diseases. Pharmaceuticals, 11(4), 93. https://doi.org/10.3390/ph11040093
33. Muhammad, M., & Huang, Q. (2021). A review of aptamer-based SERS biosensors: Design strategies and applications. Talanta, 227, 122188. https://doi.org/10.1016/j.talanta.2021.122188
34. Narayan, C., Kwon, J., Kim, C., Kim, S.-J., & Jang, S. K. (2020). Virus-based SELEX (viro-SELEX) allows development of aptamers targeting knotty proteins. The Analyst, 145(4), 1473–1482. https://doi.org/10.1039/C9AN01943J
35. Öztürk, O., Kocaman, R., & Kanbach, D. K. (2024). How to design bibliometric research: An overview and a framework proposal. Review of Managerial Science, 18(11), 3333–3361. https://doi.org/10.1007/s11846-024-00738-0
36. Park, G., Lee, M., Kang, J., Park, C., Min, J., & Lee, T. (2022). Selection of DNA aptamer and its application as an electrical biosensor for Zika virus detection in human serum. Nano Convergence, 9(1), 41. https://doi.org/10.1186/s40580-022-00332-8
37. Passas, I. (2024). Bibliometric Analysis: The Main Steps. Encyclopedia, 4(2), 1014–1025. https://doi.org/10.3390/encyclopedia4020065
38. Quaia, E., Zanon, C., Vieira, A., Loewe, C., & Marti-Bonmatí, L. (2024). Publishing in open access journals. Insights into Imaging, 15(1), 212. https://doi.org/10.1186/s13244-024-01794-6
39. Sikirzhytski, V., Virkler, K., & Lednev, I. K. (2010). Discriminant Analysis of Raman Spectra for Body Fluid Identification for Forensic Purposes. Sensors, 10(4), 2869–2884. https://doi.org/10.3390/s100402869
40. Soliman, C., Tu, D., Mabbott, S., Coté, G., & Maitland, K. (2022). Portable, multi-modal Raman and fluorescence spectroscopic platform for point-of-care applications. Journal of Biomedical Optics, 27(09). https://doi.org/10.1117/1.JBO.27.9.095006
41. Song, Z., Zhang, S., Pan, H., Hu, B., Liu, X., Cui, J., & Zhang, L. (2024). Global research trends on the links between NfL and neurological disorders: A bibliometric analysis and review. Heliyon, 10(15), e34720. https://doi.org/10.1016/j.heliyon.2024.e34720
42. Stanborough, T., Given, F. M., Koch, B., Sheen, C. R., Stowers-Hull, A. B., Waterland, M. R., & Crittenden, D. L. (2021). Optical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations. ACS Omega, 6(9), 6404–6413. https://doi.org/10.1021/acsomega.1c00008
43. Su, N., Zhang, J., Liu, W., Zheng, H., Li, M., Zhao, J., Gao, M., & Zhang, X. (2025). Specific isolation and quantification of PD-L1 positive tumor derived exosomes for accurate breast cancer discrimination via aptamer-functionalized magnetic composites and SERS immunoassay. Talanta, 281, 126956. https://doi.org/10.1016/j.talanta.2024.126956
44. Sun, Y., Ge, S., Xue, J., Zhou, X., Lu, W., Li, G., & Cao, X. (2020). Highly sensitive detection of cytochrome c in the NSCLC serum using a hydrophobic paper based–gold nanourchin substrate. Biomedical Optics Express, 11(12), 7062. https://doi.org/10.1364/BOE.408649
45. Tripathy, S., Chavva, S., Coté, G. L., & Mabbott, S. (2023). Modular and handheld Raman systems for SERS-based point-of-care diagnostics. Current Opinion in Biomedical Engineering, 28, 100488. https://doi.org/10.1016/j.cobme.2023.100488
46. Tu, D., Holderby, A., & Coté, G. L. (2020). Aptamer-based surface-enhanced resonance Raman scattering assay on a paper fluidic platform for detection of cardiac troponin I. Journal of Biomedical Optics, 25(09). https://doi.org/10.1117/1.JBO.25.9.097001
47. Tuerk, C., & Gold, L. (1990). Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase. Science, 249(4968), 505–510. https://doi.org/10.1126/science.2200121
48. Walters, R. D., McSwiggen, D. T., Goodrich, J. A., & Kugel, J. F. (2014). Selection and Characterization of a DNA Aptamer That Can Discriminate between cJun/cJun and cJun/cFos. PLoS ONE, 9(6), e101015. https://doi.org/10.1371/journal.pone.0101015
49. Yılmaz, D., Muslu, T., Parlar, A., Kurt, H., & Yüce, M. (2022). SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamers. Journal of Biotechnology, 354, 10–20. https://doi.org/10.1016/j.jbiotec.2022.06.001
50. Yu, C., Huang, Z., Ping, T., Su, H., Yang, Q., & Wu, W. (2023). Dual-mode aptasensors based on AuNPs and Ag@Au NPs for simultaneous detection of foodborne pathogens. LWT, 184, 115007. https://doi.org/10.1016/j.lwt.2023.115007
51. Yuan, K., Jurado-Sánchez, B., & Escarpa, A. (2022). Nanomaterials meet surface-enhanced Raman scattering towards enhanced clinical diagnosis: A review. Journal of Nanobiotechnology, 20(1), 537. https://doi.org/10.1186/s12951-022-01711-3
52. Zhang, H., Ye, Y., Wang, Y., Liu, J., & Jiao, Q. (2024). A Bibliometric Analysis: Current Perspectives and Potential Trends of Enzyme Thermostability from 1991–2022. Applied Biochemistry and Biotechnology, 196(3), 1211–1240. https://doi.org/10.1007/s12010-023-04615-6
53. Zhang, Y., Lai, B. S., & Juhas, M. (2019). Recent Advances in Aptamer Discovery and Applications. Molecules, 24(5), 941. https://doi.org/10.3390/molecules24050941
54. Zhang, Y., Zhang, Q., Li, Z., Zhou, X., Liu, C., Zha, H., Zhang, X., Zhao, H., Lü, G., Li, J., & Li, X. (2025). In vitro diagnosis based on SERS–LFIA: research hotspots, increase sensitivities, combined detection, multimodal detection and related patents. Journal of Materials Chemistry B, 13(20), 5746–5761. https://doi.org/10.1039/D4TB02721C
55. Zhao, L., Yang, G., Li, L., Zhu, C., Ma, Y., & Qu, F. (2020). Aptamer-functionalized magnetic nanoparticles conjugated organic framework for immobilization of acetylcholinesterase and its application in inhibitors screening. Analytica Chimica Acta, 1140, 228–235. https://doi.org/10.1016/j.aca.2020.10.024
56. Zheng, L., Ye, Q., Wang, M., Sun, F., Chen, Q., Yu, X., Wang, Y., & Liang, P. (2025). Research Progress in Small-Molecule Detection Using Aptamer-Based SERS Techniques. Biosensors, 15(1), 29. https://doi.org/10.3390/bios15010029
57. Zhou, J., Wang, D., Yang, H., & Wang, F. (2022a). Specific detection of acetamiprid with aptamer based on flexible and adhesive SERS membrane. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 270, 120801. https://doi.org/10.1016/j.saa.2021.120801
58. Zhou, Q., Jin, M., Wu, W., Fu, L., Yin, C., & Karimi-Maleh, H. (2022b). Graphene-Based Surface-Enhanced Raman Scattering (SERS) Sensing: Bibliometrics Based Analysis and Review. Chemosensors, 10(8), 317. https://doi.org/10.3390/chemosensors10080317
59. Zong, M., Zhou, L., Guan, Q., Lin, D., Zhao, J., Qi, H., Harriman, D., Fan, L., Zeng, H., & Du, C. (2021). Comparison of Surface-Enhanced Raman Scattering Properties of Serum and Urine for the Detection of Chronic Kidney Disease in Patients. Applied Spectroscopy, 75(4), 412–421. https://doi.org/10.1177/0003702820966322
60. Zou, Y., Huang, S., Liao, Y., Zhu, X., Chen, Y., Chen, L., Liu, F., Hu, X., Tu, H., Zhang, L., Liu, Z., Chen, Z., & Tan, W. (2018). Isotopic graphene–isolated-Au-nanocrystals with cellular Raman-silent signals for cancer cell pattern recognition. Chemical Science, 9(10), 2842–2849. https://doi.org/10.1039/C7SC05442D
61. Zuo, C.-J., & Tian, J. (2025). Advancing the understanding of the role of apoptosis in lung cancer immunotherapy: Global research trends, key themes, and emerging frontiers. Human Vaccines & Immunotherapeutics, 21(1). https://doi.org/10.1080/21645515.2025.2488074