Investigation of Some Hormones in Polinariums of Ophrys mammosa Desf. subsp. mammosa (Orchidaceae)

Öz

Pollen in orchids is in the form of clusters called polynarium and no hormonal studies have been found in orchid polynariums. Therefore, the aim of the study is to determine the hormones in orchid polynariums. For this purpose, Ophrys mammosa subsp mammosa polynariums collected from the field were brought to the laboratory. In the study, Salicylic acid, Abscisic acid, Jasmonic acid, Gibberellic acid and Indole-3-acetic acid hormones were screened in the samples. The samples treated with non-hydrolyzed herbal extraction were taken into the study in LC–MS/MS device. As a result of the LC–MS/MS device study, Salicylic acid was found to be 2.6736 ng/gr, Abscisic acid 65.6926 ng/gr, Jasmonic acid 12.2367 ng/gr, Gibberellic acid and Indole-3-Acetic acid were not detected. The findings are discussed in the light of current literature and it is believed that they will contribute to future orchid reproductive biology studies. The study is a first in this respect.

Anahtar Kelimeler

• Orchid
• Pollen
• Hormone
• Salicylic Acid
• Jasmonic Acid

Ophrys mammosa Desf. subsp. mammosa (Orchidaceae) Polinaryumlarında Bazı Hormonların Araştırılması

Öz

Orkidelerde polenler polinaryum denen kümeler şeklindedir ve orkide polinaryumlarında hormonal hiçbir çalışmaya rastlanmamıştır. Bu nedenle çalışmanın amacı orkide polinaryumlarındaki hormonların tespit edilmesidir. Bu amaçla araziden toplanan Ophrys mammosa subsp mammosa polinaryumları, laboratuvara getirilmiştir. Çalışmada Salisilik asit(SA), Absisik asit(ABA), Jasmonik asit(JA), Gibberellik asit(GA3) ve Indol-3-asetik(IAA)asit hormonları numunelerde taranmıştır. Hidrolize olmayan bitkisel ekstraksyon ile muamele edilen numuneler LC–MS/MS cihazında çalışmaya alınmıştır. LC–MS/MS cihaz çalışması sonucunda Salisilik asit 2,6736 ng/gr, Absisik asit 65,6926 ng/gr, Jasmonik asit 12,2367 ng/gr bulunmuş, Gibberellik asit ve Indol-3-Asetik asit tespit edilmemiştir. Tespit edilen bulgular, güncel literatürler ışığında tartışılmıştır ve ileride yapılacak orkide üreme biyolojisi çalışmalarına katkıda bulunacağına inanılmaktadır. Çalışma bu yönüyle bir ilk olma niteliğindedir.

Anahtar Kelimeler

• Orkide
• Polen
• Hormon
• Salisilik Asit
• Jasmonik Asit

Kaynakça

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