Activities of Some Hormones in Orchid Tuber Ontogeny

Öz

Orchid tubers are getting value day by day in agricultural production and also have medicinal drug importance. Since there is no hormonal research on these tubers, which are of economic value, in this study, Ophrys mammosa subsp. mammosa, it was aimed to investigate the activities of some hormones (Jasmonic acid = JA, Salicylic acid = SA, Abscisic acid = ABA) in tuber development. Tubers were taken from plants at different developmental stages (stolon emergence, stolon elongation, transformation of stolon tip to tuber and tuber maturation) and their hormone activities were examined using the LC-MS/MS method and statistical analyzes were made from three repetitive experiments. It was determined that SA, which is one of the examined hormones, increased considerably in the stages of stolon emergence, elongation, and tuber maturation, while ABA increased significantly in the stage of stolon elongation. It has been noted that ABA is more active in tuber expansion, especially with sugar movement.

Anahtar Kelimeler

• Orchid
• Tuber
• Jasmonic acid
• Salicylic acid
• Abscisic acid

Proje Numarası

TÜBAP 2020-94

Orkide Yumru Ontogenisinde Bazı Hormonların Aktiviteleri

Öz

Orkide yumruları (tuber) tarımsal üretimde gün geçtikçe değerlenmekte olup, ayrıca tıbbi drog önemine sahiptir. Ekonomik değerde olan bu yumrularla ilgili hormon içerikleri üzerine bir araştırmaya rastlanılmadığı için bu çalışmada Ophrys mammosa subsp. mammosa’da yumru gelişiminde bazı hormonların (Jasmonik asit = JA, Salisilik asit= SA, Absisik asit= ABA) aktivitelerinin araştırılması amaçlanmıştır. Yumrular, farklı gelişim aşamalarında (stolon çıkışı, stolon uzaması, stolon ucunun yumruya dönüşümü ve yumru olgunlaşması) bitkilerden alınarak LC-MS/MS yöntemi ile hormon aktiviteleri incelenmiş ve 3 tekrarlı deneylerden istatistik analizler yapılmıştır. İncelenen hormonlardan SA’nın stolonun çıkışı, uzaması ve yumru olgunlaşması aşamalarında oldukça arttığı, ABA’nın ise stolon uzaması aşamasında anlamlı düzeyde arttığı tespit edilmiştir. Yumru genişlemesinde özellikle şeker hareketi ile ABA’nın daha aktif olduğu dikkat çekmiştir.

Anahtar Kelimeler

• Orkide
• Yumru
• Jasmonik asit
• Salisilik asit
• Absisik asit

Destekleyen Kurum

Trakya Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

TÜBAP 2020-94

Teşekkür

Bu çalışma, Trakya Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından “TÜBAP 2020-94” kodlu proje ile desteklenmiştir.

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