Astaksantin'in Sıçanlarda 5-Florourasil ile İndüklenen Over Hasarı Üzerindeki Terapötik Etkisi

Öz

Astaksantin (ASX) üzerinde en çok çalışılan antioksidan moleküllerden biri olmasına rağmen, 5-florourasil (5-FU)'in neden olduğu yumurtalık hasarına karşı iyileştirici etkisi bugüne kadar gösterilmemiştir. Bu nedenle bu çalışmada ASX'in 5-FU ile indüklenen ovotoksisiteye karşı terapötik olup olmadığının araştırılması amaçlandı. Sıçanlar önce 5-FU'ya (100 mg/kg) maruz bırakıldı ve ardından üç gün boyunca ASX (250 μg/kg) tedavisi gördü. Oksidatif stres (OS), inflamasyon ve apoptoz belirteçleri spektrofotometrik yöntemlerle belirlendi. Ayrıca yumurtalık dokuları histolojik olarak da değerlendirildi. OS, inflamasyon ve apoptoz biyobelirteçlerinin seviyeleri 5-FU uygulamasıyla arttı (p<0.05). ASX ile tedavi bu belirteçleri önemli ölçüde iyileştirdi (p<0.05). Bu bulgular, ASX'in yumurtalık dokusunda pro-inflamatuar mediatörleri azaltarak ve antioksidan durumu güçlendirerek yumurtalık koruyucu bir etki gösterebileceğini ortaya koymaktadır.

Anahtar Kelimeler

• 5-florourasil
• Astaksantin
• İnflamasyon
• Oksidatif stres
• Yumurtalık dokusu hasarı

Therapeutic Effect of Astaxanthin on 5-Fluorouracil-Induced Ovarian Damage in Rats

Öz

Although astaxanthin (ASX) is one of the most studied antioxidant molecules, its curative effect against ovarian damage caused by 5-fluorouracil (5-FU) has not been demonstrated to date. It was therefore aimed to investigate whether ASX is therapeutic against 5-FU-induced ovotoxicity in this study. Rats were first exposed to 5-FU (100 mg/kg) and then treated ASX (250 μg/kg) for three days. Oxidative stress (OS), inflammation and apoptosis markers were determined using spectrophotometric methods. Ovarian tissues were also evaluated histologically. The levels of OS, inflammation and apoptosis biomarkers increased by 5-FU administration (p<0.05). Treatment with ASX significantly alleviated these markers (p<0.05). These findings reveal that ASX may exert an ovoprotective effect by reducing pro-inflammatory mediators and enhancing antioxidant status in ovarian tissue.

Anahtar Kelimeler

• 5-fluorouracil
• Astaxanthin
• Inflammation
• Ovarian damage
• Oxidative stress

Kaynakça

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