From Mass Spectrometry to Glycan Microarrays: An Overview of Analytical Techniques in Glycomics

Yıl 2024, , 218 - 235, 31.05.2024

Burcu Tekin , Rafig Gurbanov

https://doi.org/10.35193/bseufbd.1322614

Öz

Glycans, complex carbohydrate molecules, play crucial roles in various biological processes, significantly affecting health and disease. The comprehensive analysis of glycans requires a combination of advanced analytical techniques. This review provides a detailed workflow of the various methods employed in glycan analysis, including sample preparation, glycan enrichment, glycan release, labeling, separation, and detection. Each step's principles, applications, and advantages are described, highlighting their contributions to glycan research. Additionally, the review emphasizes the importance of selecting appropriate techniques for specific glycan analysis goals. The workflow provides a comprehensive understanding of glycans, unraveling their roles in biological systems and facilitating the development of novel therapeutic interventions.

Anahtar Kelimeler

Glycomics, Analytical techniques, Mass spectrometry, Liquid chromatography, Glycan microarrays

Kütle Spektrometresinden Glikan Mikrodizilerine: Glikomikte Analitik Tekniklere Genel Bir Bakış

Öz

Glikanlar, çeşitli biyolojik süreçlerde önemli rol oynayan ve sağlık ile hastalık üzerinde önemli etkileri olan karmaşık karbonhidrat molekülleri olarak bilinmektedir. Glikanların kapsamlı bir şekilde analiz edilmesi, gelişmiş analitik tekniklerin bir kombinasyonunu gerektirmektedir. Bu derleme, glikan analizinde kullanılan çeşitli tekniklerin, örnekleme hazırlığı, glikan zenginleştirme, glikan salımı, etiketleme, ayrıştırma ve tespit gibi adımlarının ayrıntılı bir iş akışını sunmaktadır. Her adımın prensipleri, uygulamaları ve avantajları açıklanarak, glikan araştırmalarına katkıları vurgulanmaktadır. Ayrıca, spesifik glikan analiz hedefleri için uygun tekniklerin seçiminin önemi üzerinde durulmaktadır. Bu iş akışı, glikanların kapsamlı bir anlayışını sağlayarak, biyolojik sistemlerdeki rollerini açığa çıkarmaya ve yeni terapötik müdahalelerin geliştirilmesine yardımcı olmaktadır.

Anahtar Kelimeler

Glikomik, Analitik teknikler, Kütle spektrometrisi, Sıvı kromatografi, Glikan mikroarrayleri

Kaynakça

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