Hızlandırılmış Çözücü Ekstraksiyonu Kullanarak Matricaria chamomilla L.'deki Fenolik Asitler ve Flavonoidler Üzerine Ekstraksiyon Sıcaklığının Etkisinin İncelenmesi

Öz

Matricaria chamomilla L., biyolojik ve terapötik özelliklerini artıran fenolik bileşikler açısından zengin, yaygın olarak kullanılan bir şifalı bitkidir. Fenolik ekstraksiyonun etkinliği ve özgüllüğü, ekstraksiyon parametrelerinden, özellikle sıcaklıktan önemli ölçüde etkilenir. Bu çalışmada, ekstraksiyon sıcaklığının Matricaria chamomilla ekstraktlarının fenolik bileşimine etkisini incelemek için hızlandırılmış çözücü ekstraksiyonu (ASE) kullanılmıştır. Ekstraksiyonlar, sabit basınç ve ekstraksiyon koşulları altında beş farklı sıcaklıkta (40, 50, 60, 80 ve 100 °C) metanol kullanılarak gerçekleştirilmiştir. Elde edilen ekstraktlar, fenolik asitlerin ve flavonoidlerin kantitatif değerlendirmesi için UHPLC–MS/MS ile incelenmiştir. Bulgular, 40 °C'de çoğu fenolik kimyasalın en yüksek konsantrasyonuna ulaşılmasıyla, sıcaklığa bağlı belirgin bir model olduğunu göstermiştir. Ekstraksiyon sıcaklığının yükseltilmesi, hem fenolik asitlerin hem de flavonoidlerin önemli ölçüde azalmasına neden olmuş, bu da termal duyarlılık ve daha yüksek sıcaklıklarda potansiyel bozulma olduğunu düşündürmüştür. 100 °C'de belirli kimyasallarda küçük miktarlarda geri kazanımlar kaydedilmiştir; ancak bu etkiler sınırlı kalmıştır. Bulgular, papatya özütlerinde fenolik bileşiklerin bütünlüğünü korumak için hafif ASE koşullarının daha uygun olduğunu göstermekte ve ASE temelli fitokimyasal ekstraksiyonda sıcaklık ayarlamasının gerekliliğini vurgulamaktadır..

Anahtar Kelimeler

• Matricaria chamomilla L
• Hızlandırılmış Çözücü Ekstraksiyonu
• LC-MS/MS
• fenolik asit
• flavonoid

Etik Beyan

Bu çalışmanın hazırlanma sürecinde bilimsel ve etik ilkelere uyulduğu ve yararlanılan tüm çalışmaların kaynakçada belirtildiği beyan olunur.

Teşekkür

Yazar, Akdeniz Üniversitesi Gıda Güvenliği ve Tarımsal Araştırmalar Merkezi'ne verdiği destek için teşekkür eder.

Investigation of the Effect of Extraction Temperature on Phenolic Acids and Flavonoids in Matricaria chamomilla L. Using Accelerated Solvent Extraction

Öz

Matricaria chamomilla L. is a commonly utilized medicinal plant abundant in phenolic compounds that enhance its biological and therapeutic attributes. The efficacy and specificity of phenolic extraction are significantly affected by extraction parameters, especially temperature. This work utilized accelerated solvent extraction (ASE) to examine the influence of extraction temperature on the phenolic composition of Matricaria chamomilla extracts. Extractions were conducted utilizing methanol at five distinct temperatures (40, 50, 60, 80 and 100 °C) under consistent pressure and extraction conditions. The resultant extracts were examined via UHPLC–MS/MS for the quantitative assessment of phenolic acids and flavonoids. The findings demonstrated a distinct temperature-dependent pattern, with the peak concentrations of the majority of phenolic chemicals achieved at 40 °C. Elevating extraction temperature resulted in a significant reduction of both phenolic acids and flavonoids, suggesting thermal sensitivity and potential destruction at higher temperatures. Minor recoveries for specific chemicals were noted at 100 °C; however, these effects were constrained. The findings indicate that mild ASE conditions are superior for maintaining phenolic integrity in chamomile extracts and emphasize the necessity of temperature adjustment in ASE-based phytochemical extraction.

Anahtar Kelimeler

• Matricaria chamomilla L
• Accelerated Solvent Extraction
• LC-MS/MS
• Phenolic acid
• Flavonoid

Etik Beyan

It is declared that scientific and ethical principles were followed during the preparation of this study and that all studies used are stated in the bibliography.

Teşekkür

The author would like to thank the Food Safety and Agricultural Research Center, Akdeniz University, for support.

Kaynakça

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