Effect of photooxidative stress and mannitol on synthesis of OmpC-OmpF proteins of Escherichia coli in lake water

Öz

This study investigated the effects of photooxidative stress and mannitol on the synthesis of OmpC-OmpF porins of Escherichia coli in lake water. The synthesis of OmpF decreased independently of photooxidative stress, whereas the synthesis of OmpC decreased as a consequence of photooxidative stress in lake water. Thus, OmpC synthesis in E. coli was directly affected by photooxidative stress. Mutations in the envZ and pta genes had no effect on the control of OmpC and OmpF synthesis in E. coli under photooxidative stress in lake water. Mannitol is an antioxidant substance that provides protection from photooxidative stress. In this study, was found that ompC expression has a regulation mechanism during photooxidative stress. Mannitol was also found to have a relationship with EnvZ in the control of porin synthesis.

Anahtar Kelimeler

• AcP,EnvZ,Lake water,Mannitol,Photooxidative

Göl suyunda Escherichia coli'nin OmpC-OmpF proteinlerinin sentezi üzerine mannitol ve fotooksidatif stresin etkisi

Öz

Bu çalışma göl suyunda Escherichia coli'nin OmpC-OmpF porinlerinin sentezi üzerine mannitol ve fotooksidatif stresin etkisini araştırmıştır. Göl suyunda OmpF sentezi fotooksidatif stresten bağımsız olarak azalmışken, OmpC sentezi fotooksidatif stresin bir sonucu olarak azalmıştır. Bu yüzden, E. coli'de OmpC sentezi  direkt olarak fotooksidatif stresten etkilenmektedir. envZ ve pta genlerindeki mutasyonların göl suyunda fotooksidatif stres altında E. coli'de OmpC ve OmpF sentezinin kontrolü üzerine bir etkisi yoktur. Mannitol fotooksidatif stresden koruyan bir antioksidant moleküldür. Bu çalışmada ompC ekspresyonu fotooksidatif stres altında bir düzenleme mekanizmasına sahiptir. Aynı zamanda mannitol'ün porin sentezinin kontrolünde EnvZ ile bir ilişkisi olduğu bulunmuştur. 

Anahtar Kelimeler

• AcP,EnvZ,Lake water,Mannitol,Fotooksidatif

Kaynakça

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