Development of Electrochemical DNA Sensor Based on Poly (Bromocresol purple) Modified Glassy Carbon Electrode for the Determination of 2-Thiouracil

Öz

In this study, the interaction between 2-thiouracil (2-TU), a potential cancer drug, and double chain deoxyribonucleic acid (dsDNA) molecule obtained from fish sperm was investigated and electrochemical determination of the drug was performed. For this, the glassy carbon electrode (GCE) surface was modified by electrochemical polymerization of the bromocresol purple (BCP) monomer and dsDNA was electrochemically immobilized (GCE/P(BCP)/dsDNA) onto the surface of this electrode (GCE/P(BCP)).  The interaction mechanism between dsDNA and 2-TU was investigated by differential pulse voltammetry method. After this interaction, a decrease in the oxidation peak current of guanine was observed and electrochemical determination of 2-TU was performed by indirect method due to this decrease. The linear operating range for 2-TU was 0.1-50 mg L^{− 1} and the detection limit was 0.033 mg L⁻¹. The interaction mechanism of 2-TU − dsDNA was also investigated by UV-Visible molecular absorption spectroscopy. The effect of the species that may cause disruptive effect on the DNA biosensor was investigated and 2-TU drug was determined in the urine sample. According to the results obtained from experimental studies, the main mode of interaction between 2-TU and dsDNA is intercalation.

Anahtar Kelimeler

• 2-Thiouracil,DNA Sensor,Poly(Bromocresol Purple),DNA-Drug Interaction

Proje Numarası

2015-41

2-Tiyourasilin Tayini için Poli(Bromokrezol moru) ile Modifiye Camsı Karbon Elektroda Dayalı Elektrokimyasal DNA Sensörü Geliştirilmesi

Öz

Bu çalışmada, potansiyel bir kanser ilacı olan 2-tiyourasil (2-TU) ilacı ile balık sperminden elde edilen çift zincirli deoksiribonükleik asit (dsDNA) molekülü arasındaki etkileşim incelenmiş ve bu etkileşime dayalı olarak ilacın elektrokimyasal tayini gerçekleştirilmiştir. Bunun için, camsı karbon elektrot (GCE) yüzeyi, bromokrezol moru (BCP) monomerinin elektrokimyasal polimerizasyonu ile modifiye edilmiş ve bu elektrot (GCE/P(BCP)) yüzeyine, dsDNA elektrokimyasal olarak immobilize edilmiştir (GCE/P(BCP)/dsDNA). dsDNA ile 2-TU arasındaki etkileşim mekanizması diferansiyel puls voltametri yöntemiyle araştırılmıştır. Bu etkileşim sonrası guaninin yükseltgenme pik akımında azalma gözlenmiş ve bu azalmaya bağlı olarak 2-TU’in elektrokimyasal tayini indirekt yöntemle gerçekleştirilmiştir. 2-TU için doğrusal çalışma aralığı 0,1-50 mg L⁻¹ ve gözlenebilme sınırı 0,033 mg L⁻¹ olarak bulunmuştur. 2-TU−dsDNA etkileşim mekanizması UV-Görünür bölge moleküler absorpsiyon spektroskopi yöntemiyle de incelenmiştir. Hazırlanan DNA biyosensörüne bozucu etki yapabilecek türlerin etkisi araştırılmış ve ayrıca 2-TU ilacının idrar numunesinde tayini gerçekleştirilmiştir. Deneysel çalışmalardan elde edilen sonuçlara göre, 2-TU ve dsDNA arasındaki başlıca etkileşim modunun interkalasyon olduğu belirlenmiştir.

Anahtar Kelimeler

• 2-Tiyourasil,DNA Sensörü,Poli(bromokrezol moru),DNA-ilaç etkileşimi

Destekleyen Kurum

Kütahya Dumlupınar Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

2015-41

Teşekkür

Yazarlar 2015-41 nolu proje ile destek sağlayan Kütahya Dumlupınar Üniversitesi Bilimsel Araştırma Projeleri Birimine ve Burcu Demir’e yüksek lisans tez dönemi boyunca Yurtiçi Öncelikli Alanlar Yüksek Lisans Burs Programı (2210-C) kapsamında burs sağlayan Türkiye Bilimsel ve Teknolojik Araştırma Kurumuna (TÜBİTAK) teşekkür ederler.

Kaynakça

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