ZmMPK14’ün Bitki Savunmasında Görev Aldığının Mukayeseli Ekspresyon Analiziyle Belirlenmesi

Öz

Mitojenle-Aktive olan Protein Kinazlar (MAPKs) stimulusla aktive olan sensörlerle yanıt mekanizmaları arasında sinyal iletiminde kaskadlar halinde fonksiyonel olan moleküllerdir. Bu kaskadların son basamağında bir grup C MAPK olan ZmMAPK14 duyarlı ve dirençli genotipi cDNA-AFLP çalışmalarında diferansiyel ekspresyon gösteren bir mesaj olarak tanımlanmıştır: Puccinia sorghi (Ps) inokülasyonuyla dirençli genotipte göreceli bir indüksiyon görülürken, duyarlı genotipte belirgin bir represyon tespit edilmiştir. RT-qPCR çalışmaları diğer taraftan Ps inokülasyonuyla her iki genotipte farklı düzeylerde indüksiyon olduğunu göstermiştir: Duyarlı genotipte kontrole göre 1.5- 2.5 kat arasında değişim gösteren bir ekspresyon artışı görülürken dirençli genotipte 12. h’de 149 kat ile başlayan ve 72. h’de 477 kat tepe değerine ulaşılan dramatik göreceli bir artış gözlenmiştir. Söz konusu MAP Kinazın bitki savunmasında rolüyle ilgili daha somut bulgular elde etmek için, kitin, Salisilik Asit (SA) ve H2O2 uygulamalarıyla ZmMapk14 ekspresyonunda değişim her iki genotipte 0-24 aralığını kapsayan altı örnekleme noktasında çalışılmıştır. Tüm uygulamalar dirençli genotipte istatistiki önemli indüksiyonlar ortaya çıkarırken, duyarlı genoptipte ekspresyon değişimleri Ps uygulamasındakine benzer düzede kompleks ve sınırlı olarak tespit edilmiştir. Sonuçlar, ZmMAPK14 ekspresyonunun gerçekleştirilen uygulamalarla değişim gösterdiğini ve Ps’e karşı bitki savunmasında fonksiyonel olduğunu göstermektedir. 

Anahtar Kelimeler

• ZmMAPK14
• Bitki Savunması
• SA
• Kitin
• H2O2

Involvement of ZmMPK14 in Plant Defense Revealed by Comparative Expression Analysis

Öz

Mitogen-Activated Protein Kinases (MAPKs) function in signaling pathways as modules cascading between stimulus activated sensors and response mechanisms.ZmMAPK14, a group C final MAPK of this cascade was identified as a differentially expressed message in cDNA-AFLP studies of both susceptible and resistant genotypes, where a gradual induction was displayed in the resistant genotype while a clear repression occurred in the susceptible genotype in response to Puccinia sorghi (Ps) inoculation. RT-qPCR verification studies, however, did not reveal the same pattern of expression in that both displayed inductions at different levels. Ps inoculation induced a limited expression increase fluctuating between 1.5 and 2.5-fold in the susceptible genotype while a dramatic upregulation starting at 12 h with a 149-fold and gradually increasing to a maximum level of 477 folds at 72 h in resistant genotype was observed. To obtain further evidence about its role in plant defense, ZmMapk14 expression in response to applications of chitin, Salicylic Acid (SA) and H2O2 at six time points covering a 0-24 h interval were studied in both genotypes. All treatments induced ZmMapk14 expression in the resistant genotype significantly at different levels while the expressional changes in the susceptible were more complex and limited similar to the Ps inoculation levels in the susceptible. Overall, the results show that ZmMAPK14 display differential expression in resistant and susceptible genotypes in response to Ps inoculation and applied defense compounds, and it presumably functions in plant defense to Ps.

Anahtar Kelimeler

• ZmMAPK14
• Plant Defense
• SA
• Chitin
• H2O2

Kaynakça

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