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Determination of Bacteria by Impedance Measurement Approach Using Proteinoids from Biological Recognition Elements

Yıl 2021, Cilt: 8 Sayı: 2, 668 - 679, 31.12.2021
https://doi.org/10.35193/bseufbd.949812

Öz

In today's world, with the developing technology and the increase in water, food and environmental pollution, the health control of these basic needs is quite challenging. There has been a study on the elimination of such deficiencies. For this purpose, a two-stage business plan was created. First; Proteininoids, which are biomolecules that react selectively to target bacteria, were synthesized and characterized. Unlike the biological recognition elements used up to now, proteinoids were used in this study for the first time. In the second stage, proteinoid selectively detects spreading bacteria and cause a change in electrical impedance and physical parameters, thereby providing bioelectric transformation. Diagnostic performance in bacterial stimulation was achieved with electrical characterization methods in the transducer.

Kaynakça

  • Bilal, M. & Iqbal, H. (2019). Microbial-derived biosensors for monitoring environmental contaminants: Recent advances and future outlook. Process Safety and Environment Protection, 90,1-5
  • Dincer, C., Bruch, R., Costa-Rama, E., Fernández-Abedul, M. T., Merkoçi, A., Manz, A., Urban, G. A. & Güder, F. (2015). Disposable Sensors in Diagnostics, Food, and Environmental Monitoring. Advanced Materials, 1806739
  • Gavrilescu, M., Demnerova, K., Aamand, J., Agathos, S. & Fava, F.(2015). Emerging pollutants in the environment: present and future challenges in biomonitoring, ecological risks and bioremediation, New Biotechnology, 32, 1.
  • Chae, M. S., Yoo, Y. K., Kim, J., Kim, T. G. & Hwang, K. S. (2018). Graphene-based enzyme-modified field-effect transistor biosensor for monitoring drug effects in Alzheimer’s disease treatment. Sensors & Actuators: B. Chemical, 272, 448–458
  • Dincer, C., Bruch, R., Costa-Rama, E., Fernández-Abedul, M. T., Merkoçi, A., Manz, A., Urban, G. A. & Güder, F. (2019). Disposable Sensors in Diagnostics, Food, and Environmental Monitoring. Advanced Materials, 1806739
  • Cesewski, E. & Johnson, B. N. (2020). Electrochemical biosensors for pathogen detection. Biosensors and Bioelectronics, 159, 112214
  • Zhenguo, Z., Jun, Z. & Xin, D. (2019). Electrochemical Biosensors for Detection of Foodborne Pathogens. Micromachines, MDPI, 10, 222
  • Bhalla, N., Jolly, P., Formisano, N. & Estrela, P. (2016). Introduction to biosensors. Essays in Biochemistry, 60, 1–8
  • Nakashima, T. & Fox, S. W. (1980). Synthesis of Peptides from Amino Acids and ATP with Lysine-Rich Proteinoid. Journal of Molecular Evolution, 15, 161-168
  • Lugasi, L., Grinberg, I. & Margel, S. (2020). Targeted Delivery of CBD-Loaded Poly (RGD) Proteinoid Nanoparticles for Antitumor Therapy, Journal of Nanomed Nanotechonology, 11(6), 552.
  • Kumar, A. B. M., Jayakumar, R. & Rao, K. P. (1996). Synthesis and Aggregational Behavior of Acidic Proteinoid, Journal of Polymer Science: Part A: Polymer Chemistry, 34, 2915-2924
  • Sasson, E., Ruth, V., Margel, S. & Klipcan, L. (2020). Engineering and use of proteinoid polymers and nanocapsules containing agrochemicals. Scientific RepoRtS Nature, 10, 9171
  • Tallawi, M. (2010). Proteinoid/hydroxyapatite hybrid microsphere composites’ Wıley Perıodıcals.
  • Hadad, E. Rudnick-Glick, S. Grinberg, I. Yehuda, R. & Margel, S. (2020). Engineering of NIR fluorescent PEGylated poly(RGD) proteinoid polymers and nanoparticles for drug delivery applications in chicken embryo and mouse models’, Royal Society of Chemistry, 10, 34364
  • Matsuno, K. (1982). Natural self-organization of polynucleotides and polypeptides in protobiogenesis: Appearance of a protohypercycle, BioSystems, 15, 1-11
  • Kolitz‐Domb, M., & Margel, S. (2018). Recent Advances of Novel Proteinoids and Proteinoid Nanoparticles and Their Applications in Biomedicine and Industrial Uses, Israel Journal Chemistry, 58, 1277.
  • Madhan, K. A. B., & Panduranga, R. (1998). Preparation and characterization of pH-sensitive proteinoid microspheres for the oral delivery of methotrexate. Biomaterials, 19, 725-732
  • Kwon, K., Park, D., & Kim, J. C. (2019). Disulfide proteinoid micelles responsive to reduction, Journal of Dispersion Science and Technology, 40(10), 1413-1422
  • Lugasi, L. Grinberg, I. Rudnick‑Glick, S. Okun, E. Einat, H., & Margel, S. (2020). Designed proteinoid polymers and nanoparticles encapsulating risperidone for enhanced antipsychotic activity. Journal of Nanobiotechnol, 18, 149
  • Madhan, K. A. Jayakumar, R., & Panduranca, K. R. (1996). Synthesis and Aggregational Behavior of Acidic Proteinoid, Journal of Polymer Science: Part A: Polymer Chemistry, 34, 2915-2924
  • Kumar, A. B. M. & Rao, K. P. (1998). Preparation and characterization of pH-sensitive proteinoid microspheres for the oral delivery of methotrexate”, Biomaterials, 19, 725-732

Biyolojik Tanıma Elemanlarından Proteinoidler Kullanılarak Empedans Ölçüm Yaklaşımıyla Bakterilerin Tespiti

Yıl 2021, Cilt: 8 Sayı: 2, 668 - 679, 31.12.2021
https://doi.org/10.35193/bseufbd.949812

Öz

Günümüz dünyasında gelişen teknoloji ile su, gıda ve çevresel kirliliğin artmasıyla bu temel ihtiyaçların sağlık kontrolü oldukça zorlayıcıdır. Bu tür eksiklerin giderilmesi üzerine bir çalışma olmuştur. Bu amaçla iki temel aşamalı bir iş planı oluşturulmuştur. Birincisi; hedef bakteriye seçici tepki veren biyomoleküllerden olan proteinoidler sentezlenip karakterize edilmiştir. Şuana kadar kullanılan biyolojik tanıma elemanlarından ilk defa farklı olarak proteinoidler bu çalışma ile kullanılmıştır. İkinci aşamada ise proteinoidler, yayılım gösteren bakterileri seçici olarak algılayarak elektriksel empedans ile fiziksel parametrelerde değişime neden olarak bioelektrik dönüşümü sağlanmasıdır. Dönüştürücü (transducer) içerisinde elektriksel karakterizasyon yöntemleri ile bakteri uyarımlarında teşhis başarımı elde edilmiştir.

Kaynakça

  • Bilal, M. & Iqbal, H. (2019). Microbial-derived biosensors for monitoring environmental contaminants: Recent advances and future outlook. Process Safety and Environment Protection, 90,1-5
  • Dincer, C., Bruch, R., Costa-Rama, E., Fernández-Abedul, M. T., Merkoçi, A., Manz, A., Urban, G. A. & Güder, F. (2015). Disposable Sensors in Diagnostics, Food, and Environmental Monitoring. Advanced Materials, 1806739
  • Gavrilescu, M., Demnerova, K., Aamand, J., Agathos, S. & Fava, F.(2015). Emerging pollutants in the environment: present and future challenges in biomonitoring, ecological risks and bioremediation, New Biotechnology, 32, 1.
  • Chae, M. S., Yoo, Y. K., Kim, J., Kim, T. G. & Hwang, K. S. (2018). Graphene-based enzyme-modified field-effect transistor biosensor for monitoring drug effects in Alzheimer’s disease treatment. Sensors & Actuators: B. Chemical, 272, 448–458
  • Dincer, C., Bruch, R., Costa-Rama, E., Fernández-Abedul, M. T., Merkoçi, A., Manz, A., Urban, G. A. & Güder, F. (2019). Disposable Sensors in Diagnostics, Food, and Environmental Monitoring. Advanced Materials, 1806739
  • Cesewski, E. & Johnson, B. N. (2020). Electrochemical biosensors for pathogen detection. Biosensors and Bioelectronics, 159, 112214
  • Zhenguo, Z., Jun, Z. & Xin, D. (2019). Electrochemical Biosensors for Detection of Foodborne Pathogens. Micromachines, MDPI, 10, 222
  • Bhalla, N., Jolly, P., Formisano, N. & Estrela, P. (2016). Introduction to biosensors. Essays in Biochemistry, 60, 1–8
  • Nakashima, T. & Fox, S. W. (1980). Synthesis of Peptides from Amino Acids and ATP with Lysine-Rich Proteinoid. Journal of Molecular Evolution, 15, 161-168
  • Lugasi, L., Grinberg, I. & Margel, S. (2020). Targeted Delivery of CBD-Loaded Poly (RGD) Proteinoid Nanoparticles for Antitumor Therapy, Journal of Nanomed Nanotechonology, 11(6), 552.
  • Kumar, A. B. M., Jayakumar, R. & Rao, K. P. (1996). Synthesis and Aggregational Behavior of Acidic Proteinoid, Journal of Polymer Science: Part A: Polymer Chemistry, 34, 2915-2924
  • Sasson, E., Ruth, V., Margel, S. & Klipcan, L. (2020). Engineering and use of proteinoid polymers and nanocapsules containing agrochemicals. Scientific RepoRtS Nature, 10, 9171
  • Tallawi, M. (2010). Proteinoid/hydroxyapatite hybrid microsphere composites’ Wıley Perıodıcals.
  • Hadad, E. Rudnick-Glick, S. Grinberg, I. Yehuda, R. & Margel, S. (2020). Engineering of NIR fluorescent PEGylated poly(RGD) proteinoid polymers and nanoparticles for drug delivery applications in chicken embryo and mouse models’, Royal Society of Chemistry, 10, 34364
  • Matsuno, K. (1982). Natural self-organization of polynucleotides and polypeptides in protobiogenesis: Appearance of a protohypercycle, BioSystems, 15, 1-11
  • Kolitz‐Domb, M., & Margel, S. (2018). Recent Advances of Novel Proteinoids and Proteinoid Nanoparticles and Their Applications in Biomedicine and Industrial Uses, Israel Journal Chemistry, 58, 1277.
  • Madhan, K. A. B., & Panduranga, R. (1998). Preparation and characterization of pH-sensitive proteinoid microspheres for the oral delivery of methotrexate. Biomaterials, 19, 725-732
  • Kwon, K., Park, D., & Kim, J. C. (2019). Disulfide proteinoid micelles responsive to reduction, Journal of Dispersion Science and Technology, 40(10), 1413-1422
  • Lugasi, L. Grinberg, I. Rudnick‑Glick, S. Okun, E. Einat, H., & Margel, S. (2020). Designed proteinoid polymers and nanoparticles encapsulating risperidone for enhanced antipsychotic activity. Journal of Nanobiotechnol, 18, 149
  • Madhan, K. A. Jayakumar, R., & Panduranca, K. R. (1996). Synthesis and Aggregational Behavior of Acidic Proteinoid, Journal of Polymer Science: Part A: Polymer Chemistry, 34, 2915-2924
  • Kumar, A. B. M. & Rao, K. P. (1998). Preparation and characterization of pH-sensitive proteinoid microspheres for the oral delivery of methotrexate”, Biomaterials, 19, 725-732
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Asiye Yurttaş 0000-0002-6424-7411

Yayımlanma Tarihi 31 Aralık 2021
Gönderilme Tarihi 8 Haziran 2021
Kabul Tarihi 29 Eylül 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 8 Sayı: 2

Kaynak Göster

APA Yurttaş, A. (2021). Biyolojik Tanıma Elemanlarından Proteinoidler Kullanılarak Empedans Ölçüm Yaklaşımıyla Bakterilerin Tespiti. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, 8(2), 668-679. https://doi.org/10.35193/bseufbd.949812