Activities of Some Hormones in Orchid Tuber Ontogeny

Yıl 2024, Cilt: 11 Sayı: 1, 19 - 25, 31.05.2024

Mehmet Aybeke

https://doi.org/10.35193/bseufbd.1205766

Öz

Orchid tubers are getting value day by day in agricultural production and also have medicinal drug importance. Since there is no hormonal research on these tubers, which are of economic value, in this study, Ophrys mammosa subsp. mammosa, it was aimed to investigate the activities of some hormones (Jasmonic acid = JA, Salicylic acid = SA, Abscisic acid = ABA) in tuber development. Tubers were taken from plants at different developmental stages (stolon emergence, stolon elongation, transformation of stolon tip to tuber and tuber maturation) and their hormone activities were examined using the LC-MS/MS method and statistical analyzes were made from three repetitive experiments. It was determined that SA, which is one of the examined hormones, increased considerably in the stages of stolon emergence, elongation, and tuber maturation, while ABA increased significantly in the stage of stolon elongation. It has been noted that ABA is more active in tuber expansion, especially with sugar movement.

Anahtar Kelimeler

Orchid, Tuber, Jasmonic acid, Salicylic acid, Abscisic acid

Proje Numarası

TÜBAP 2020-94

Orkide Yumru Ontogenisinde Bazı Hormonların Aktiviteleri

Öz

Orkide yumruları (tuber) tarımsal üretimde gün geçtikçe değerlenmekte olup, ayrıca tıbbi drog önemine sahiptir. Ekonomik değerde olan bu yumrularla ilgili hormon içerikleri üzerine bir araştırmaya rastlanılmadığı için bu çalışmada Ophrys mammosa subsp. mammosa’da yumru gelişiminde bazı hormonların (Jasmonik asit = JA, Salisilik asit= SA, Absisik asit= ABA) aktivitelerinin araştırılması amaçlanmıştır. Yumrular, farklı gelişim aşamalarında (stolon çıkışı, stolon uzaması, stolon ucunun yumruya dönüşümü ve yumru olgunlaşması) bitkilerden alınarak LC-MS/MS yöntemi ile hormon aktiviteleri incelenmiş ve 3 tekrarlı deneylerden istatistik analizler yapılmıştır. İncelenen hormonlardan SA’nın stolonun çıkışı, uzaması ve yumru olgunlaşması aşamalarında oldukça arttığı, ABA’nın ise stolon uzaması aşamasında anlamlı düzeyde arttığı tespit edilmiştir. Yumru genişlemesinde özellikle şeker hareketi ile ABA’nın daha aktif olduğu dikkat çekmiştir.

Anahtar Kelimeler

Orkide, Yumru, Jasmonik asit, Salisilik asit, Absisik asit

Destekleyen Kurum

Trakya Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

TÜBAP 2020-94

Teşekkür

Bu çalışma, Trakya Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından “TÜBAP 2020-94” kodlu proje ile desteklenmiştir.

Kaynakça

• Leake, J. R. (2005). Plants parasitic on fungi: unearthing the fungi in myco-heterotrophs and debunking the ‘saprophytic’ plant myth. Mycologist, 19, 113-122.
• Attri, L. K., Bhanwra R. K. & Nayyar, H. (2020). Pollination induced embryology studies in Aerides multiflora (ROXB.). International Journal of Botanical Studies, 5(4), 211–215.
• Güler, N. & Deniz, İ. G. (2012). Orchidaceae. Türkiye Bitkileri Listesi (Damarlı Bitkiler), 1.baskı. Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Basımı, İstanbul, 630-652.
• Cozzolino, S. & Widmer, A. (2005). Orchid diversity: an evolutionary consequence of deception? Trends in Ecology & Evolution, 20, 487-494.
• Stern, W. L. (1997). Vegetative anatomy of subtribe Orchidinae (Orchidaceae). Botanical Journal of the Linnean Society,124, 121–136.
• Aybeke, M., Sezik, E. & Olgun, G. (2010). Vegetative anatomy of some Ophrys, Orchis and Dactylorhiza (Orchidaceae) taxa in Trakya region of Turkey. Flora, 205, 73–89.
• Aybeke, M. (2012). Comparative anatomy of selected rhizomatous and tuberous taxa of subfamilies Orchidoideae and Epidendroideae (Orchidaceae) as an aid to identification. Plant Systematic & Evolution, 298, 1643–1658.
• Sezik, E. (1984). Orkidelerimiz, Türkiye’nin Orkideleri. Sandoz Kültür Yayınları, No: 6. İstanbul.
• T.C. Orman ve Su İşleri Bakanlığı Orman Genel Müdürlüğü. (2014). Salep Eylem Planı, 2014-2018. https://web.ogm.gov.tr/ekutuphane/Yayinlar/Salep%20Eylem%20Plan%C4%B1.pdf. (12.11.2014)
• Aksenova, N. P., Konstantinova, T. N., Golyanovskaya, S. A., Sergeeva, L. I. & Romanov, G. A. (2012). Hormonal regulation of tuber formation in potato plants. Russian Journal of Plant Physiology, 59, 4, 451–466.
• Doganlar, Z. B. (2012). Physiological and genetic responses to pesticide mixture treatment of Veronica beccabunga. Water Air & Soil Pollution, 223, 6201–6212.
• Mokronosov, A.T. (1990). Tuberization and Source-Sink Relations in Potato, Regulyatsiya rosta i razvitiya kartofelya (Regulation of Potato Growth and Development). Chailakhyan, M.Kh. and Mokronosov, A.T., eds. Regulation of Potato Growth and Development, Nauka, Moscow, 6-12.
• Chun‑Juan, D., Xin‑Yan, L., Lu‑Lu, X., Ling‑Ling, W. & Qing‑Mao, S. (2020). Salicylic acid regulates adventitious root formation via competitive inhibition of the auxin conjugation enzyme CsGH3.5 in cucumber hypocotyls. Planta, 252, 75.
• Klerk, G. J. De, Guan, H. Y., Huisman, P. & Marinova, S. (2011). Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus ‘Jork 9’. Plant Growth & Regulation, 63, 175–185.
• Wen Xu, Y,. Shuai Shuai, Lv., Zhao, D., Wen Chen, J., Ting Yang, W. & Wu, W. (2012). Effects of salicylic acid on monoterpene production and antioxidant systems in Houttuynia cordata. African Journal of Biotechnology, 11, 1364–1372.
• Mutlu-Durak H. & Kutman, Y. (2021). Seed treatment with biostimulants extracted from weeping willow (Salix babylonica) enhances early maize growth. Plants, 10, 1449.
• Zhou, D., Shen, W., Cui, Y., Liu, Y., Zheng, X., Li, Y., Wu, M., Fang, S., Liu, C., Tang, M., Yi, Y., Zhao M. & Chen, L. (2021). Apical spikelet abortion (asa) controls apical panicle development in rice by regulating salicylic acid biosynthesis. Frontiers in Plant Science, 12, 636877.
• Miura, K. & Tada, Y. (2014). Regulation of water, salinity, and cold stress responses by salicylic acid. Frontiers in Plant Science, 5, 4.
• Dixit, R., Agrawal, L., Srivastava S. & Chauhan, P. S. (2021). Paenibacillus lentimorbus enhanced abiotic stress tolerance through lateral root formation and phytohormone regulation. Journal of Plant Growth Regulation. https://doi.org/10.1007/s00344-021-10439-7.
• Macháková, I., Konstantinova, T. N., Sergeeva, L. I., Lozhnikova, V. N., Golyanovskaya, S. A., Dudko, N. D., Eder, J. & Aksenova, N. P. (1998). Photoperiodic control of growth, development and phytohormone balance in Solanum tuberosum. Physiologia Plantarum, 102, 272–278.
• Aksenova, N. P., Sergeeva, L. I., Kolachevskaya O. O. & Romanov, G. A. (2014). Hormonal regulation of tuber formation in potato. Ramawat, K. G. and Merillon, J. M. ed. 2013. Bulbous plants, Biotechnology. CRC Press, New York, 3-36.
• Orman-Ligeza, B., Parizot, B., Rycke, R. De, Fernandez, A., Himschoot, E., Breusegem F. Van, Bennett, M. J., Périlleux, C., Beeckman, T. & Draye, X. (2016). RBOH-mediated ROS production facilitates lateral root emergence in Arabidopsis. Development, 143, 3328–3339.
• Waszczak, C., Carmody M. & Kangasjarvi, J. (2018). Reactive oxygen species in plant signaling. Annual Review of Plant Biology, 69, 209–236.
• Xu, L., Zhao, H. Y., Ruan, W. Y., Deng, M. J., Wang, F., Peng J. R., Luo, J., Chen, Z. & Yi, K. (2017). Abnormal inflorescence meristem1 functions in salicylic acid biosynthesis to maintain proper reactive oxygen species levels for root meristem activity in rice. Plant Cell, 29, 560–574.
• Mady, M. (2009). Effect of foliar application with salicylic acid and vitamin E on growth and productivity of tomato (Lycopersicon esculentum, Mill.) Plant. Journal of Plant Production, 34, 6715–6726.
• La, V. H., Lee, B. R., Islam, M. T., Park, S. H., Jung, H. I., Bae D. W. & Kim, T. H. (2019). Characterization of salicylic acid-mediated modulation of the drought stress responses: Reactive oxygen species, proline, and redox state in Brassica napus. Environmental and Experimental Botany, 157, 1–10.
• Gao, W., Liu, Y., Huang, J., Chen, Y., Chen, C., Lu, L., Zhao, H., Men, S. & Zhang, X. (2021). MES7 modulates seed germination via regulating salicylic acid content in Arabidopsis. Plants,10, 903.
• Wenting, G., Niu, L., Wang, C., Wei, L., Pan Y. & Liao, W. (2021). Hydrogen peroxide is ınvolved in salicylic acid‑induced adventitious rooting in cucumber under cadmium stress. Journal of Plant Biology. https://doi.org/10.1007/s12374-021-09332-3
• Ravi, V., Chakrabarti, S. K., Makeshkumar, T. & Saravanan, R. (2014). Molecular regulation of storage root formation and development in sweet potato. Hoboken, N.J. ed. Horticultural Reviews, vol. 42, Wiley, USA, 157–208.
• Jing, F. U., Xu, Y. J., Lu, C., Yuan, L. M., Wang, Z. Q. & Yang, J. C. (2013). Changes in enzyme activities involved in starch synthesis and hormone concentrations in superior and inferior spikelets and their association with grain filling of super rice. Rice Science, 2, 120–128.
• Park, S. H., Lee, B. R., La, V. H., Mamun, M. A., Bae D. W. & Kim, T. H. (2021). Drought intensity-responsive salicylic acid and abscisic acid crosstalk with the sugar signaling and metabolic pathway in Brassica napus. Plants, 10, 610.
• He, X. L., Shi, L. W., Yuan, Z. H., Xu, Z., Zhang, Z. Q. & Ming-Fang, Y. (2008). Effects of lipoxygenase on the corm formation and enlargement in Gladiolus hybridus. Scientia Horticulturae, 118, 60–69.
• Sarkar, D., Pandey, S. K. & Sharma, S. (2006). Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro. Plant Cell, Tissue and Organ Culture, 87, 285–295.