The Impact of Temperature on the Synthesis of Silver Nanoparticles by Candida macedoniensis

Yıl 2023, Cilt: 6 Sayı: 1, 163 - 166, 01.01.2023

Mirmusa Jafarov Khudaverdi Ganbarov Ergin Kariptaş , Sanam Huseynova Sevinj Gulıyeva

https://doi.org/10.19127/bshealthscience.1189775

Cited By: 1

Öz

Nanoparticles are widely used in medical diagnosis and treatment, as carriers of drug preparations, in cosmetics, production packaging and transportation of foods and etc. Special attention is paid to the use of biological structures in the production of nanoparticles. The aim at the presented work was to investigate the influence of temperature on the formation of silver nanoparticles by Candida macedoniensis BSU-MI44. Wet biomass of yeast at AgNO3 solution, was incubated at 25, 30, 35, 40°C. The samples have been analyzed on the UV spectrometer, the scanning electron microscope and the X-ray spectroscope. Spectrophotometric analyses showed 410 nm wavelength (peak), characteristic for silver nanoparticles in samples incubated at 25 and 30°C. By increasing temperature, the formation of silver nanoparticles has weakened and has finally stopped. The optimum temperature was between 25-30°C for the production of silver nanoparticles and the formed nanoparticles were spherical at both temperatures. The sizes of silver nanoparticles formed at 25°C and 30°C were 65.6 and 14.2-22.9 nm. The sizes of the first ones have been 2.8-4.7 times larger than the sizes of the others. Correspondingly X- ray spectroscopic analyses of the obtained samples showed the characteristic absorption peak for silver nanoparticles formed at temperatures 25 and 30°C.

Anahtar Kelimeler

Candida macedoniensis, Silver nanoparticles, UV spectrum, SEM, X-ray spectrum

Kaynakça

• Abo–State MAM, Partila AM. 2015. Microbial production of silver nanoparticles by Pseudomonas aeruginosa. Cell free extract. J Eco Heal Env, 3(3): 91–98.
• Anal KJha, Prasad K, Kulkarni AR. 2008. Yeast mediated synthesis of silver nanoparticles. Inter J Nanosci Nanotech, 4(1): 17-21.
• Bhainsa KC, D'Souza SF. 2006. Biomimetic synthesis of nanoparticles. Colloids Surf B, 47: 160–164.
• Bharde A, Rautaray D, Bansal V, Ahmad A, Sarkar I, Mohammad Yusuf S, Sanyal M, Sastry M. 2006, Extracellular biosynthesis of magnetite using fungi. Small, 2(1): 135-141.
• Egorova EM, Revina AA. 2000. Synthesis of metallic nanoparticles in reverse micelles in the presence of quercetin. Colloids and Surfaces A: Physicochem Engin Aspects, 168: 87-96.
• Ganbarov KhG, Jafarov MM, Hajiyeva FT, Bozkurt HC, Ramazanov MA, Eyvazova GI, Aghamaliyev ZA, Ahmedov IS, Abdulhamidova SM. 2015a. The study of the ability of Candida macedoniensis BSU-MI44 to synthesize silver nanoparticles. J Qafqaz Univ Chem Biol, 2015: 139–142.
• Ganbarov KhG, Jafarov MM, Hajiyeva FT and Bozkurt HC. 2015b. Studying of capacity of strains of yeast fungus Candida macedoniensis BDU-Mİ44 to synthesize nanoparticles of silver. Modern Mycology in Russia. In Proceeding of Materials of III International Mycological Forum, Moscow, Russia, 4: 82.
• Ganbarov KhG, Jafarov MM, Hajiyeva FT, Bozkurt HC, Ramazanov MA, Eyvazova GI. 2016a. The formation of silver nanoparticles by Candida macedoniensis BSU-MI44 depending on the amount of biomass. In Proceeding of International Scientific-Practical Conference On Actual Problems Of Modern Chemistry And Biology, May 12, Ganja, Azerbaijan, 2016:161–165.
• Ganbarov KhG, Jafarov MM, Hajiyeva FT, Bozkurt HC, Ramazanov MA, Eyvazova QI, Aghamaliyev ZA, Akhmedov IS. 2016b. Mycogenic Formation of Silver Nanoparticles by the Azerbaijanese environmental isolate Candida macedoniensis BDU–Mİ44. Inter J Res Stud in Biosci, 5: 1–5.
• Ganbarov KhG, Musayev EM. 2012. Microorganisms forming nanoparticles. Transaction of the Institute of Microbiol Azerbaijan National Acad, 10: 78–84.
• Kowshik M, Ashtaputre SH, Kharazi SH, Vogel W, Urban J, Kulkarni SK, Paknikar KM. 2003. Extracellular synthesis of silver nanoparticles by a silver- tolerant yeast strain MKY3 Nanotechnol, 14(1): 95 – 100.
• Li X, Xu H, Chen ZS, Chen G. 2011. Biosynthesis of nanoparticles by microorganisms and their applications. J Nanomaterial, 11(8): 1–17.
• Muthupandian S, Amelash T, Negash L, Gebreyesus A, Selvaraj A, Rayar V and Dheekonda K. 2013. Extracellular biosynthesis and biomedical application of silver nanoparticles synthesized from baker’s yeast. Inter J Res Pharma Biomed Sci, 4(3):822 – 828.
• Narayanan KB and Sakthivel N. 2010. Biological synthesis of metal nanoparticles by microbes. Advances in Colloid Interface Sci, 156 ( 1,2): 1–13.
• Sadowski Z, Maliszewska IH, Grochowalska B, Polowczyk I, and Koźlecki T. 2008. Synthesis of silver nanoparticles using microorganisms. Materials Sci, 26: 2419–2424.
• Sadowski Z. 2010. Biosynthesis and application of silver and gold nanoparticles. Wroclaw Univ Technol, 11: 257–276.
• Sastry M, Ahmad A, Khan MI, Kumar R. 2003. Biosynthesis of metal nanoparticles using fungi and actinomycete. Current Sci, 85: 162–170.