Identification of virulence resistance genes in Pseudomonas aeruginosa strains isolated from blood samples

Yıl 2022, , 64 - 69, 30.04.2022

İlknur Bıyık , Yeliz Tanrıverdi Çaycı , Ege Berke Atıgan , Asuman Bırıncı

https://doi.org/10.34084/bshr.1090884

Öz

Aim: In this it is aimed to the determine the presence of virulence resistance genes (toxA, algD, plcN, lasB, plcH) in P. aeruginosa isolates isolated from blood samples.
Materials and methods: DNA extraction of the study isolates was done by boiling method. Optimization was done using positive control after DNA extraction. After optimization, the presence of virulence (toxA, algD, plcN, lasB, plcH) resistance genes was investigated by polymerase chain reaction (PCR) method.
Results: As a result of PCR of the virulence gene regions (toxA, algD, lasB, plcN, plcH); Positive rates of KR 25 isolates were 80% (n=20), 100% (n=25), 100% (n=25), 100% (n=25), 96% (n=24) in KS 46 isolates. On the other hand, the positive rate distributions were found to be 93.47% (n=43), 100% (n=46), 100% (n=46), 100% (n=46), 97.82% (n=45).
Conclusion: As a result of PCR of virulence gene regions (toxA, algD, lasB, plcN, plcH) of P. aeruginosa, it was determined that algD, lasB, plcN genes were found in all 25 carbapenem resistant (KR) and 46 carbapenem sensitive (KS) isolates.

Anahtar Kelimeler

P. aeruginosa, virulence, bactaremia

Kan örneklerinden izole edilen Pseudomonas aeruginosa suşlarında virülans direnç genlerinin belirlenmesi

Öz

Amaç: Kan örneklerinden izole edilen P. aeruginosa izolatlarındaki virülans direnç genlerinin (toxA, algD, plcN, lasB, plcH) varlığının belirlenmesidir.
Yöntem: Çalışma izolatlarının DNA ekstraksiyonu kaynatma yöntemiyle yapıldı. DNA ekstraksiyonundan sonra pozitif kontrol kullanılarak optimizasyon yapıldı. Optimizasyondan sonra virülans (toxA, algD, plcN, lasB, plcH) direnç genlerinin varlığının polimeraz zincir reaksiyonu (PZR) yöntemi ile araştırıldı.
Bulgular: Yapılan virülans gen bölgelerinin (toxA, algD, lasB, plcN, plcH) PZR işlemi sonucunda; karbapenem dirençli (KR) 25 izolatta pozitif oranları sırasıyla % 80 (n=20), % 100 (n=25), % 100 (n=25), % 100 (n=25), %96 (n=24) olduğu, karbapenem duyarlı (KS) 46 izolatta ise pozitif oran dağılımlarının % 93,47 (n=43), % 100 (n=46), % 100 (n=46), % 100 (n=46), %97,82 (n=45) olduğu saptanmıştır.
Sonuç: P. aeruginosa’ın virülans gen bölgelerinin (toxA, algD, lasB, plcN, plcH) PZR işlemi sonucunda KR 25 ve KS 46 izolatların hepsinde algD, lasB, plcN genlerinin bulunduğu belirlenmiştir.

Anahtar Kelimeler

P. aeruginosa, Virülans, bakteriyemi

Kaynakça

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