Molecular Diagnostic Tests in Microbiota Investigations

Öz

In the recent years, a plenty of data has been acquired about the potential effects of the microbiota on the human health, and strong evidences have been appeared related to the roles of the microbiota on the pathophysiology of some diseases. This situation has led many medical disciplines to pay attention to the human microbiome, and the number of researches on this topic has signifi cantly increased. Therefore, a requirement has emerged for the medical microbiology laboratories that previously used to work on the pathogen-based identifi cations, to identify hundreds of non-pathogenic bacteria, fungi and viruses present in the human body fl oras. The secrets of microbiome have been expressed as we have effectively used the available techniques among the culture-based and molecular-based identifi - cation methods. In this review, it is aimed to share the recent knowledge about the molecular-based investigation tools with evaluating the available laboratory research methods that can be utilized in the microbiota studies.

Anahtar Kelimeler

• Microbiota,Culture,Molecular Diagnostic Methods

Mikrobiyota Çalışmalarında Moleküler Tanı Yöntemleri

Öz

Son yıllarda mikrobiyotanın insan sağlığı üzerine olan potansiyel etkileri hakkında çok daha fazla veri elde edilmekte ve bazı hastalıkların patofi zyolojisinde mikrobiyotanın rol oynadığına dair güçlü bulgulara ulaşılmaktadır. Bu durum, birçok farklı tıp branşının insan mikrobiyotomuna ilgi duymasına neden olmuş ve bu konudaki çalışmalar önemli düzeyde artmıştır. Böylelikle; günümüze kadar genellikle sadece patojen-odaklı olarak çalışan tıbbi mikrobiyoloji laboratuvarları için insan fl orasında bulunan ve patojen olmayan yüzlerce bakteri, mantar ve virüsün tanımlanması gerekliliği ortaya çıkmıştır. Sahip olduğumuz tanı yöntemleri içinde kültür ve moleküler temelli yöntemlerin bu alanda etkili olarak kullanılması ile mikrobiyotomun sırları açıklanmaya başlanmıştır. Bu derlemede, mikrobiyota çalışmalarında kullanılan laboratuvar tanı yöntemleri değerlendirilerek moleküler esaslı inceleme araçları hakkındaki güncel verilerin paylaşılması amaçlanmıştır.

Anahtar Kelimeler

• Mikrobiyota,Kültür,Moleküler Tanı Yöntemleri

Kaynakça

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