Antioxidant Variability of Propolis Collected from Different Zones in Hives

Öz

Propolis is biologically highly active honey bee product. The popularity of propolis is increasingly growing because of its contribution to human health. Propolis composition is highly variable depending on its sources. Different honey bees subspecies can collect propolis having different anti-bacterial effect. Honey bees, collect propolis for a couple of different purposes such as narrowing the entrance of their own hives and airflow isolation. In this study, propolis collected from entrances (EC) and top of deep supper (FC) of six different hives, and the antioxidant properties of these propolis samples were compared. Average values of total phenolic content were 68.5 and 62.6 mg GAE/g propolis extract, IC50 value of DPPH were 0.14 and 0.16 mg/mL, and FRAP value were 43.5 and 38.4 mg TE/g propolis extract samples collected from EC and FC, respectively. Statistically significant differences have not been found in terms of antioxidant activity analysis between EC and FC collected propolis (P>0.05).

Anahtar Kelimeler

• Total phenolic content
• DPPH
• FRAP

Kaynakça

1. Ahmed, R., Tanvir, E. M., Hossen, M., Afroz, R., Ahmmed, I., Rumpa, N. E., Sudip, P., Siew, H.G., Siti, A. S., & Khalil, M. (2017). Antioxidant properties and cardioprotective mechanism of Malaysian propolis in rats. Evidence-Based Complementary and Alternative Medicine, 5370545.
2. Aliyazıcıoglu, R., Sahin, H., Erturk, O., Ulusoy, E., & Kolayli, S. (2013). Properties of phenolic composition and biological activity of propolis from Turkey. International Journal of Food Properties, 16(2), 277-287.
3. Bankova, V., Christov, R., Kujumgiev, A., Marcucci, M. C., & Popov, S. (1995). Chemical composition and antibacterial activity of Brazilian propolis. Zeitschrift für Naturforschung C, 50(3-4), 167-172.
4. Barlak, Y., Değer, O., Ucar, M., & Çakıroğlu, T. N. (2015). Effects of Turkish propolis extract on secretion of polymorphonuclear elastase following respiratory burst. Turkish Journal of Biology, 39(2), 194-201.
5. Bonvehí, J. S., & Coll, F. V. (1994). The composition, active components and bacteriostatic activity of propolis in dietetics. Journal of the American Oil Chemists’ Society, 71(5), 529-532.
6. Elmastas, M., Isildak, O., Turkekul, I., & Temur, N. (2007). Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. Journal of Food Composition and Analysis, 20(3-4), 337–345.
7. Ghisalberti, E. L. (1979). Propolis: a review. Bee World, 60 (2), 59-84.
8. Gonzalez, R., Remirez, D., Rodriguez, S., Gonzalez, A., Ancheta, O., Merino, N., & Pascual, C. (1994). Hepatoprotective effects of propolis extract on paracetamol‐induced liver damage in mice. Phytotherapy Research, 8(4), 229-232.

9. Greenaway, W., Scaysbrook, T., & Whatley, F. R. (1990). The composition and plant origins of propolis: a report of work at Oxford. Bee World, 71(3), 107-118.
10. Gülçin, Ì., Şat, İ. G., Beydemir, Ş., Elmastaş, M., & Küfrevioǧlu, Ö. İ. (2004). Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb) buds and lavender (Lavandula stoechas L.). Food Chemistry, 87(3), 393-400.
11. Krol, W., Scheller, S., Czuba, Z., Matsuno, T., Zydowicz, G., Shani, J., & Mos, M. (1996). Inhibition of neutrophils' chemiluminescence by ethanol extract of propolis (EEP) and its phenolic components. Journal of Ethnopharmacology, 55(1), 19-25.
12. Lachman, J., Orsák, M., Hejtmánková, A., & Kovářová, E. (2010). Evaluation of antioxidant activity and total phenolics of selected Czech honeys. LWT - Food Science and Technology, 43(1), 52–58.
13. Marcucci, M. C. (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26(2), 83-99.
14. Marghitas, L. A., Dezmirean, D., Moise, A., Mihai, C., & Laslo, L. (2009). DPPH Method for evaluation of propolis antioxidant activity. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, 66(1-2).
15. Matsushige, K., Kusumoto, I. T., Yamamoto, Y., Kadota, S., & Namba, T. (1995). Quality evaluation of propolis 1. A comparative study on radical scavenging effects of propolis and vespae nidus. Journal of Traditional Medicine, 12, 45-53.
16. Mercan, N., Kivrak, I., Duru, M. E., Katircioglu, H., Gulcan, S., Malci, S., ... & Salih, B. (2006). Chemical composition effects onto antimicrobial and antioxidant activities of propolis collected from different regions of Turkey. Annals of Microbiology, 56(4), 373.
17. Mitamura, T., Matsuno, T., Sakamoto, S., Maemura, M., Kudo, H., Suzuki, S., ... & Nagasawa, H. (1996). Effects of a new clerodane diterpenoid isolated from propolis on chemically induced skin tumors in mice. Anticancer Research, 16(5A), 2669-2672.
18. Oyaizu. M. (1986). Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition,44, 307-315.
19. Ozdal, T., Sari-Kaplan, G., Mutlu-Altundag, E., Boyacioglu, D., & Capanoglu, E. (2018). Evaluation of Turkish propolis for its chemical composition, antioxidant capacity, anti-proliferative effect on several human breast cancer cell lines and proliferative effect on fibroblasts and mouse mesenchymal stem cell line. Journal of Apicultural Research, 57(5), 627-638.
20. Popova, M., Antonova, D., & Bankova, V. (2017). Chemical composition of propolis and American foulbrood: Is there any relationship?. Bulgarıan Chemıcal Communıcatıons, 49, 171-175.
21. Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry, 40(6), 945-948.
22. Silici, S., & Kutluca, S. (2005). Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. Journal of Ethnopharmacology, 99(1), 69-73.
23. Slinkard, K., & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49-55.
24. Socha, R., Gałkowska, D., Bugaj, M., & Juszczak, L. (2015). Phenolic composition and antioxidant activity of propolis from various regions of Poland. Natural Product Research, 29(5), 416-422.
25. Talla, E., Tamfu, A. N., Gade, I. S., Yanda, L., Mbafor, J. T., Laurent, S., Luce, V.E., Milena, P., & Bankova, V. (2017). New mono-ether of glycerol and triterpenes with DPPH radical scavenging activity from Cameroonian propolis. Natural Product Research, 31(12), 1379-1389.