Flow Sitometri ve Bitki Islahı

Yıl 2020, Cilt: 34 Sayı: 1, 213 - 223, 01.06.2020

Fatih Demirel Barış Eren Serap Demirel Aybüke Erol

Öz

Bitkiler hem insanoğlunun hem de hayvanların beslenmesinde önemli bir yere sahiptir. Artan dünya popülasyonu ve küresel ısınma besin kalitesi ve kaynağı üzerinde problemler oluşturmaktadır. Bitki ıslahı besin kalitesinin ve verimin arttırılması amacıyla birçok bitkide uygulanmıştır. Flow sitometri (flow cytometry; FCM) keşfedildiğinden beri bitki ıslahında ploidi seviyelerinin belirlenmesi için uygun ve kullanışlı bir araç olmuştur. Flow sitometri ebeveyn bitkilerin karakterize edilmesi, çaprazlama sonucu oluşan yavru bitkilerin kromozom içeriğinin saptanması, poliploidizasyon çalışmaları gibi farklı amaçlar doğrultusunda kullanılmaktadır. Bu çalışma da flow sitometri ve bitki ıslahında kullanımı ele alınmıştır. Teknolojinin gelişmesi ile birlikte flow sitometrinin tarımda ve bitki ıslahında kullanımı yaygınlaşmıştır. Araştırmacıların genom modifikasyonu üzerindeki çalışmalara ilgisi arttıkça, flow sitometrinin farklı uygulamalarda kullanılacağı olası gözükmektedir. 

Anahtar Kelimeler

Bitki, DNA miktarı, FCM, ıslah

Flow Cytometry and Plant Breeding

Öz

Plants have a cruial place in the nutrition of both the humankind and the animals. Increasing world population and global warming create problems on food quality and sources. Plant breeding has been applied in many plants in order to increase nutrient quality and yield. Since flow cytometry was discovered, it has been a convenient and useful tool for determining ploidy levels in plant breeding. Flow cytometry is used for different purposes such as characterization of parent plants, determination of chromosome content of offspring plants and polyploidization studies in breeding. In this study flow cytometry and its use in plant breeding are discussed. With the development of technology and automated systems, the use of flow cytometry in agriculture and plant breeding has become widespread. As researchers have increased interest in genome modification studies, it is likely that flow cytometry will be used in different applications.

Anahtar Kelimeler

breeding, DNA amount, FCM, plant

Kaynakça

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