Farklı Amerikan Asma Anaçlarında Kurşun Stresi Üzerine Salisilik Asit Uygulamalarının Etkileri

Yıl 2022, Cilt: 36 Sayı: 1, 129 - 156, 01.06.2022

Selda Daler , Emine Sema Çetin , Salih Seren

https://doi.org/10.20479/bursauludagziraat.948894

Öz

Salisilik asit (SA), bitkilerde biyotik ve abiyotik stres kaynaklı birçok fizyolojik tepkiye aracılık eden önemli bir sinyal molekülüdür. Bu çalışmada, faklı konsantrasyonlarda kurşun [Pb(NO3)2] stresine maruz bırakılan 5 BB, 41 B ve 1103 P Amerikan asma anaçlarında değişen dozlardaki SA uygulamalarının morfolojik, fizyolojik ve biyokimyasal özellikler üzerine etkileri incelenmiştir. Dikimden 6 hafta sonra 0, 1.0, 2.5 ve 5.0 mM dozlardaki SA, bitkilerin tüm yeşil aksamına pülverizasyon yöntemiyle; 0, 10, 25 ve 50 ppm konsantrasyonlardaki Pb(NO3)2, bitki kök bölgesine enjeksiyon yöntemiyle uygulanmıştır. Elde edilen sonuçlar, farklı konsantrasyonlarda Pb(NO3)2 ve SA uygulamalarına yanıt olarak her üç anaçta da incelenen özellikler bakımından farklılıklar olduğunu göstermektedir. Köklenme oranı bakımından en etkili uygulamanın 5.0 mM SA konsantrasyonu olduğu belirlenirken, fiziksel zararlanma derecesi ve membran zararlanma derecesinin azaltılmasında tüm SA konsantrasyonlarının etkili olduğu tespit edilmiştir. Klorofil miktarının, 5 BB ve 1103 P anaçlarında artan SA konsantrasyonlarına paralel olarak artış gösterdiği; fenolik madde içeriğinin 5 BB ve 41 B anaçlarında 2.5 mM SA konsantrasyonunda en yüksek değere ulaştığı, prolin miktarının 5 BB anacında, 1.0 mM; 41 B anacında, 2.5 ve 5.0 mM; 1103 P anacında ise 1.0, 2.5 ve 5.0 mM SA konsantrasyonlarında azalma gösterdiği ve sürgün uzunluğunun ise 5 BB anacında, 5.0 mM SA konsantrasyonuyla en yüksek değeri aldığı belirlenmiştir. Aynı zamanda köklenme oranı ile toplam fenolik madde miktarı; fiziksel zararlanma derecesi ile klorofil miktarı ve membran zararlanma derecesi ile klorofil miktarı arasında önemli düzeyde ancak negatif yönde bir ilişki olduğu saptanmıştır. Çalışmada ekzojen SA uygulamalarının, bitkilerde Pb(NO3)2 varlığından kaynaklanan oksidatif stresi hafifletme bakımından etkili bir uygulama olduğu ve SA aktivitesinin, Amerikan asma anaçlarının türlerine bağlı olarak değişiklik gösterdiği sonucuna varılmıştır.

Anahtar Kelimeler

Anaç, ağır metal, kurşun stresi, asma, salisilik asit

Destekleyen Kurum

TÜBİTAK 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı (2019/2)

Proje Numarası

1919B011904222

Teşekkür

Yazarlar finansal desteği için 1919B011904222 Başvuru Numaralı 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programına (2019/2) teşekkür ederler.

Effects of Salicylic Acid Application on Lead Stress in Different American Grapevine Rootstocks

Öz

Salicylic acid (SA) is an important signalling molecule that mediates many physiological responses from biotic and abiotic stress-induced in plants. In this study, the effects on morphological, physiological and biochemical properties of varying doses of SA applications in 5 BB, 41 B and 1103 P American grapevine rootstocks exposed to different concentrations of lead [Pb(NO3)2] stress were investigated. Six weeks after planting, 0, 1.0, 2.5 and 5.0 mM doses of SA were applied onto the entire green surface of the plant by the pulverisation method; 0, 10, 25 and 50 ppm concentrations of Pb(NO3)2 were applied by the injection method to the plant root zone. The results show that there were differences in the examined properties in all three rootstocks in response to Pb(NO3)2 and SA applications at different concentrations. It was determined that the most effective application in terms of rooting rate 5.0 mM SA concentration, determined that all SA concentrations effective in reducing the degree of physical damage and the degree of membrane damage. It was found that the amount of chlorophyll increased in parallel with the rising SA concentrations in 5 BB and 1103 P rootstocks; It determined that phenolic content reached the highest value at 2.5 mM SA concentration in 5 BB and 41 B rootstocks, the amount of proline decreased in SA concentrations of 1.0 mM in 5 BB rootstocks, 2.5 and 5.0 mM in 41 B rootstocks, and 1.0, 2.5 and 5.0 mM in 1103 P rootstocks. It was determined that 5.0 mM SA concentration increased shoot length in 5 BB rootstocks. Simultaneously, it was determined that there a significant but negative correlation between rooting rate with total phenolic substance content, the degree of physical damage with the amount of chlorophyll and the degree of membrane damage with the amount of chlorophyll. In the study, it was concluded that exogenous SA applications an effective application in terms of alleviating the oxidative stress caused by the presence of Pb(NO3)2 in plants, and the activity of SA varies depending on the species of American grapevine rootstocks.

Anahtar Kelimeler

heavy metal, lead stress, salicylic acid, grapevine, rootstock

Proje Numarası

1919B011904222

Kaynakça

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