Phylogenetic Analysis of Mosquito (Diptera: Culicidae) Species with Mitochondrial Cytochrome Oxidase Subunit 1 Gene Distributed in Kocaeli

Bu makalenin ilk hali 30 Haziran 2021 tarihinde yayımlandı. https://dergipark.org.tr/tr/pub/cbayarfbe/article/1007398

Düzeltme Notu

Acknowledgement section was revised as follows: We would like to thank the Scientific Research Project Unit of Kocaeli University (Project number: 2019-042). Additional thanks to Ela Gök for proofreading this article.

Öz

Mosquitoes, which are in the Culicidae family and have blood-sucking properties, infect humans and animals with many diseases. In the present study, it was aimed that mosquitoes spreading in the Kocaeli province were detected species identification based on DNA barcoding method. DNA isolation was performed with the samples of mosquitoes collected in the Kocaeli province between June 2017 and September 2018. Then, Polymerase Chain Reaction (PCR) and DNA sequence analysis were applied by using universal primers of the mitochondrial COI gene. The sequences in FASTA format obtained with the Chromas program were compared with those of other mosquito species in the world through the NCBI-BLAST database. For phylogenetic analysis, the sequences were uploaded into the MEGA X program, and phylogenetic trees were created in the Maximum Likelihood method, Tamura-Nei Model (Tamura & Nei, 1993), Bootstrap 1000. Among mosquitoes collected in this study, 7 species belonging to Aedes, Anopheles, Culiseta and Culex genera were identified and characterized as Aedes geniculatus (n = 10), Aedes albopictus (n = 7), Anopheles funestus (n = 1), Anopheles plumbeus (n = 1), Culiseta longiareolata (n = 1), Cx. torrentium (n = 1) and Cx. pipiens complex sp. (n = 33). Within them, Cx. pipiens complex sp. was found as the dominant species in Kocaeli. In conclusion, this study revealed the first molecular research of mosquito species spreading in Kocaeli and provided records to GenBank.

Anahtar Kelimeler

• Aedes
• Anopheles
• Culex
• Culiseta
• COI

Kaynakça

1. Kettle DS (1995). Medical and veterinary entomology. 2nd ed., CAB International, Wallingford, UK.
2. Günay F (2015). Türkiye sivrisinek faunası üzerine dna barkodlama yöntemiyle moleküler analizler. Doktora Tezi, Hacettepe Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
3. Ergunay K, Gunay F, Erisoz Kasap O, Oter K, Gargari S, Karaoglu T, Tezcan S, Cabalar M, Yildirim Y, Emekdas E, Alten B and Ozkul A (2014). Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile Virus in Turkey. PLoS Neglected Tropical Diseases; 8, e3028.
4. Şahingöz Demirpolat G, İnci A, Yıldırım A, Düzlü Ö, Önder Z (2018). Kayseri yöresinde Culex pipiens biyotipleri ve Culex torrentium’un real time PCR ile araştırılması ve moleküler karakterizasyonu, Ankara Universitesi Veteterinerlik Fakültesi Dergisi; 65 (3), 253-259.
5. Öter K, Tüzer E (2014). İstanbul’da sivrisinek türlerinin (Diptera: Culicidae) kompozisyonu. İstanbul Üniversitesi Veterinerlik Fakültesi Dergisi; 40 (2), 249-259.
6. Çetin H, Yanıkoğlu A (2004). Antalya kentinde bulunan sivrisinek (Diptera: Culicidae) türleri, üreme alanları ve baskın tür Culex pipiens L.’in bazı özellikleri. Türk Entemoloji Dergisi; 28, 283-294.
7. Demirci B, Lee Y, Lanzaro GC, Alten B (2012). Identification and characterization of single nucleotide polymorphisms (SNPs) in Culex theileri (Diptera: Culicidae). Journal of Medical Entomology; 49(3), 581-588.
8. Topluoğlu S, Karasartova D, Karaer ZK, Taylan-Özkan A (2020). Şanlıurfa yöresindeki anofel larvalarının morfolojik tanımlanması ve üreme alanlarının fiziksel ve ekolojik özelliklerinin araştırılması. Türk Hijyen ve Deneysel Biyoloji Dergisi; 77(2), 207-216.

9. URL1-Harbach RE. Genus Anopheles Meigen, 1818. Mosquito Taxonomic Inventory, 2015. http://mosquito-taxonomic-inventory.info/genus-anopheles-meigen-1818# (Acces Date: 02/08/2021)
10. Bişkin Z, İnci A, Yıldırım A, Düzlü Ö (2010). Kayseri’nin Felahiye yöresinde yaygınlık gösteren sivsisinek (Diptera: Culicidae) türleri. Sağlık Bilimleri Dergisi; 19(2), 133-139.
11. Kuclu O, Dik B (2018). Mosquito (Diptera: Culicidae) fauna of Western Black Sea Region of Turkey. Türkiye Parazitoloji Dergisi; 42(2), 138-43.
12. Sarıkaya Y, (2017). Türkiye-Suriye sınırında yer alan mülteci kampları çevresinde ve mülteci göç yolları üzerindeki sivrisinek (Diptera: Culicidae) türlerinin tespit edilmesi. Yüksek Lisans Tezi, Hacettepe Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
13. Tuna Türkozan S (2019). Türkiye’de Aedes (Stegomyia) albıpictus (Scue, 1894)’un populasyon genetiği ve ekolojik niş modellemesi. Yüksek Lisans Tezi. Aydın Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü, Aydın.
14. Öter K (2007). İstanbul’da görülen sivrisinek türlerinin tespiti. Doktora Tezi. İstanbul Üniversitesi, Sağlık Bilimleri Enstitüsü, İstanbul.
15. Ortman BD, Bucklin A, Pages F, Youngbluth M (2010). DNA barcoding the medusozoa using mtCOI. Deep-Sea Research II; 57(24-26), 2148-2156.
16. URL-2: https://www.genome.jp/tools-bin/clustalw (Acces Date: 10/31/2019)
17. Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution; 35(6), 1547-1549.
18. Tamura K (1992): Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution; 9(4), 678-687.
19. Tamura K, Nei M (1993): Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution; 10(3), 512-526.
20. Korkmaz S, Yıldırım A, Düzlü Ö, Çiloğlu A, Önder Z, İnci A (2016). Kayseri yöresinden toplanmış culex pipiens komplekse ait sivrisinek (Diptera: Culicidae) örneklerinin kan beslenme identifikasyonu, Türkiye Parazitol Dergisi; 40, 199-204.
21. Laurito M, Oliveira TM, Almirón W.R, Sallum MAM, (2013). COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil. Memorias do Instituto Oswaldo Cruz; 108(suppl 1), 110-122.
22. Shaikevich EV, Vinogradova E., Bouattour A, Almeida APG (2016). Genetic diversity of Culex pipiens mosquitoes in distinct populations from Europe: contribution of Culex quinquefasciatus in Mediterranean populations. Parasites Vectors; 9, 47.
23. Danabalan R, Ponsonby DJ, Lintoni YM (2012). A critical assessment of available molecular identification tools for determining the status of Culex pipiens s.l. in the United Kingdom, Journal of the American Mosquito Control Association; 28(4 suppl), 68–75.
24. Morçiçek B, Gocmen Taskin B, Doğaç E, Doğaroğlu T, Taskin V (2018). Evidence of natural Wolbachia infections and molecular identification of field populations of Culex pipiens Complex (Diptera: Culicidae) mosquitoes in Western Turkey, Journal of Vector Ecology; 43(1), 44-51.
25. Hebert PD, Cywinska A, Ball SL, deWaard JR (2003). Biological identifications through DNA barcodes. Proceeding Biological Sciences/The Royal Society; 270(1512): 313–321.
26. Hebert, PD, Ratnasingham S, J.R. deWaard JT (2003). Barcoding animal life: COI divergences among closely allied species. Proceeding Biological Sciences/The Royal Society;270: S96-S99.
27. Günay F, Alten B, Simsek F, Aldemir A, Linton YM (2015). Barcoding Turkish Culex mosquitoes to facilitate Arbovirus Vector incrimination studies reveals hidden diversity and new potential vectors. Acta Tropica; 143, 112-120.
28. Şahin, İ (1984). Antalya ve çevresindeki sivrisinekler (Diptera: Culicidae) ve filariose vektörü olarak önemleri üzerinde araştırmalar. II. Sivrisinek Faunasını Belirlemek Amacıyla Yapılan Çalışmalar. Doğa Bilimleri Dergisi A2, 8: 385-396.
29. Şakacı Z (2018). Edirne merkez ilçesinde vektöriyel öneme sahip sivrisinek (Diptera: Culicidae) türlerinin araştırılması, Yüksek Lisans Tezi, Trakya Üniversitesi, Fen Bilimleri Enstitüsü, Edirne.
30. Öter K, Günay F, Tüzer E, Linton YM, Bellini R, Alten B (2013). First record of Stegomyia albopicta in Turkey determined by active ovitrap surveillance and dna barcoding. Vector Borne and Zoonotic Diseases; 13(10), 753-761.
31. Akiner MM, Demirci B, Babuadze G, Robert V, Schaffner F (2016). Spread of the invasive mosquitoes Aedes aegypti and Aedes albopictus in the Black Sea Region increases risk of Chikungunya, Dengue, and Zika Outbreaks in Europe. PLoS Neglected Tropical Diseases; 10(5), e0004664.
32. Şakacı Z (2021). Contribution to mosquito (Diptera: Culicidae) fauna of Sakarya province and the first record of the invasive vector Aedes albopictus (Skuse, 1894) for Kocaeli province. BAUN Fen Bilimleri Enstitüsü Dergisi; 23(1), 10-21.
33. Schaffner F, Thiéry I, Kaufmann C, Zettor A, Lengeler C, Mathis A, Bourgoin C (2012). Anopheles plumbeus (Diptera: Culicidae) in Europe: a Mere Nuisance Mosquito or Potential Malaria Vector? Malaria Journal, 11, 393.
34. Muslu H (2009): Manisa bölgesinde saptanan sivrisinek türleri (Dipteria: Culicidae) ve bunların vektöryel önemleri. Yüksek Lisans Tezi, Celal Bayar Üniversitesi, Fen Bilimleri Enstitüsü, Manisa.