Anthemis cotula'dan Fenolik ve Flavonoid Bileşiklerinin Ultrason Destekli Ekstraksiyonu: Antioksidan Potansiyel Değerlendirmesi

Öz

Tıbbi bitkilerde etkili özütleme yöntemleri ve biyolojik etkinlik potansiyelinin araştırılması, doğal ürün araştırmalarında önemli bir odak noktası olmaya devam etmektedir. Bu çalışmanın amacı, Anthemis cotula L.'den ultrason-destekli özütleme yöntemi kullanılarak elde edilen metanol ve su özütlerinin kimyasal bileşimini ve antioksidan özelliklerini değerlendirmektir. Özütler, özüt verimi, toplam fenolik madde içeriği (TPC), toplam flavonoid içeriği (TFC) ve altı farklı analizle antioksidan aktiviteleri açısından karakterize edilmiştir: CUPRAC, FRAP, fosfomolibden, DPPH, ABTS ve demir iyonu şelatlama aktivitesi. Su özütü (%19.99), metanol özütüne (11.43%) kıyasla daha yüksek bir verim göstermiştir. Ancak, metanol özütü (56.27 mg GAEs/g ekstrakt ve 27.98 mg REs/g ekstrakt), su özütüne kıyasla daha yüksek TPC ve TFC değerlerine sahip olup, sırasıyla 64.33 mg GAEs/g özüt ve 39.22 mg REs/g özüt olarak ölçülmüştür (p < 0.05). Antioksidan analizleri, metanol özütünün daha güçlü bir indirgeme gücüne sahip olduğunu göstermiştir; CUPRAC ve FRAP değerleri sırasıyla; 262.83 ve 154.98 mg TEs/g özüt iken, su özütünün değerleri 235.98 ve 127.53 mg TEs/g özüt olarak bulunmuştur. DPPH ve ABTS radikal temizleme aktiviteleri özütler arasında benzerlik göstermiş olup, IC50 değerleri benzer etkinlikleri işaret etmiştir. Önemli olarak, su özütü, demir iyonu şelatlama aktivitesinde (12.30 mg EDTAEs/g özüt, IC50 = 1.06 mg/mL) metanol özütünü (8.55 mg EDTAEs/g özüt, IC50 = 1.53 mg/mL) geride bırakmıştır. Bu bulgular, A. cotula özütlerinin özellikle metal iyonlarını şelatlama ve indirgeme gücü sergileme potansiyelini vurgulamaktadır. Gelecekteki çalışmalar, bu aktivitelerden sorumlu biyolojik olarak aktif bileşenlerin izole edilmesine ve bu bileşenlerin farmasötik veya nütrosötik formülasyonlardaki potansiyel uygulamalarına odaklanabilir.

Anahtar Kelimeler

• Anthemis cotula
• Ultrason Destekli Ekstraksiyon
• Fenolik Bileşikler
• Antioksidan Aktivite

Ultrasound Assisted Extraction of Phenolic and Flavonoid Compounds from Anthemis cotula: Antioxidant Potential Assessment

Öz

The exploration of effective extraction methods and bioactive potential in medicinal plants remains a critical focus for natural product research. This study aimed to evaluate the chemical composition and antioxidant properties of methanol and water extracts obtained from Anthemis cotula L. using ultrasound-assisted extraction. The extracts were characterized for extraction yield, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities via six assays: CUPRAC, FRAP, phosphomolybdenum, DPPH, ABTS, and ferrous ion chelating activity. The water extract exhibited a higher yield (19.99%) compared to the methanol extract (11.43%). However, the methanol extract demonstrated superior TPC and TFC, with values of 64.33 mg GAEs/g extract and 39.22 mg REs/g extract, respectively, compared to 56.27 mg GAEs/g extract and 27.98 mg REs/g extract for the water extract (p < 0.05). Antioxidant assays revealed that the methanol extract possessed stronger reducing power, with CUPRAC and FRAP values of 262.83 and 154.98 mg TEs/g extract, respectively, compared to 235.98 and 127.53 mg TEs/g extract for the water extract. DPPH and ABTS radical scavenging activities were comparable between extracts, with IC50 values indicating similar efficacy. Notably, the water extract excelled in ferrous ion chelating activity (12.30 mg EDTAEs/g extract, IC50 = 1.06 mg/mL), outperforming the methanol extract (8.55 mg EDTAEs/g extract, IC50 = 1.53 mg/mL). These findings underscore the potential of A. cotula extracts, particularly in chelating metal ions and exhibiting reducing power. Future studies may focus on isolating bioactive compounds responsible for these activities and exploring their applications in pharmaceutical or nutraceutical formulations.

Anahtar Kelimeler

• Anthemis cotula
• Ultrasound assisted extraction
• Phenolic compounds
• Antioxidant activity

Kaynakça

1. [1]. Verpoorte, R.; van der Heijden, R. T. J. M.; Ten Hoopen, H. J. G.; Memelink, J. 1999. Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals. Biotechnology Letters; 21: 467-479. (doi.org /10.1023/A:1005502632053).
2. [2]. Gómez-Galera, S.; Pelacho, A. M.; Gené, A.; Capell, T.; Christou, P. 2007. The genetic manipulation of medicinal and aromatic plants. Plant Cell Reports; 26: 1689-1715. (doi.org/ 10.1007/s00299-007-0384-x).
3. [3]. Kumar, J.; Gupta, P. K. 2008. Molecular approaches for improvement of medicinal and aromatic plants. Plant Biotechnology Reports; 2: 93-112. (doi.org/ 10.1007/s11816-008-0059-2).
4. [4]. Joshi, K.; Chavan, P.; Warude, D.; Patwardhan, B. 2004. Molecular markers in herbal drug technology. Current Science; 87(2): 159-165.
5. [5]. Bardaweel, S. K.; Tawaha, K. A.; Hudaib, M. M. 2014. Antioxidant, antimicrobial and antiproliferative activities of Anthemis palestina essential oil. BMC Complementary and Alternative Medicine; 14: 1-8. (doi.org/10.1186/1472-6882-14-297).
6. [6]. Al-Snafi, A. E. 2016. Medical importance of Anthemis nobilis (Chamaemelum nobile)-a review. Asian Journal of Pharmaceutical Science & Technology; 6(2): 89-95.
7. [7]. Ugurlu, E.; Secmen, O. 2008. Medicinal plants popularly used in the villages of Yunt Mountain (Manisa-Turkey). Fitoterapia; 79(2): 126-131.(doi.org/10.1016/j.fitote.2007.07.016).
8. [8]. Ozkaynak, S.; Kolatan, H. E.; Yilmaz, O.; Kivcak, B. 2011. Anti-inflammatory activity of Anthemis aciphylla var. aciphylla Boiss. Turkish Journal of Biology; 35(6): 757-762. (doi.org/10.3906/biy-1006-15).

9. [9]. Shawahna, R.; Jaradat, N. A. 2017. Ethnopharmacological survey of medicinal plants used by patients with psoriasis in the West Bank of Palestine. BMC Complementary and Alternative Medicine; 17: 1-9. (doi:10.1186/s12906-016-1503-4).
10. [10]. Sut, S.; Dall’Acqua, S.; Zengin, G.; Senkardes, I.; Bulut, G.; Cvetanović, A. et al. 2019. Influence of different extraction techniques on the chemical profile and biological properties of Anthemis cotula L.: Multifunctional aspects for potential pharmaceutical applications. Journal of Pharmaceutical And Biomedical Analysis; 173: 75-85.( doi: 10.1016/j.jpba.2019.05.028).
11. [11]. Sarikurkcu, C.; Ozer, M. S.; Cakir, A.; Eskici, M.; Mete, E. 2013. GC/MS evaluation and in vitro antioxidant activity of essential oil and solvent extracts of an endemic plant used as folk remedy in Turkey: Phlomis bourgaei Boiss. Evidence-Based Complementary and Alternative Medicine; 2013: Article ID 293080, 7 pages. (doi: 10.1155/2013/293080).
12. [12]. Zengin, G.; Sarikurkcu, C.; Gunes, E.; Uysal, A.; Ceylan, R.; Uysal, S. et al. 2015. Two Ganoderma species: Profiling of phenolic compounds by HPLC-DAD, antioxidant, antimicrobial and inhibitory activities on key enzymes linked to diabetes mellitus, Alzheimer's disease and skin disorders. Food and Function; 6(8): 2794-2802.(doi: 10.1039/c5fo00665a)
13. [13]. Kocak, M. S.; Sarikurkcu, C.; Cengiz, M.; Kocak, S.; Uren, M. C.; Tepe, B. 2016. Salvia cadmica: Phenolic composition and biological activity. Ind. Crops Prod.; 85: 204-212.(doi.org/10.1016/j.indcrop.2016.03.015).
14. [14]. Apak, R.; Güçlü, K.; Özyürek, M.; Esin Karademir, S.; Erçaǧ, E. 2006. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int. J. Food Sci. Nutr.; 57(5-6): 292-304. (doi: 10.1080/09637480600798132).
15. [15]. Odabas Kose, E.; Aktaş, O.; Deniz, I. G.; Sarikürkçü, C. 2010. Chemical composition, antimicrobial and antioxidant activity of essential oil of endemic Ferula lycia Boiss. Journal of Medicinal Plants Research; 4(17): 1698-1703.(doi: 10.5897/JMPR09.439).
16. [16]. Zengin, G.; Sarikurkcu, C.; Uyar, P.; Aktumsek, A.; Uysal, S.; Kocak, M. S. et al. 2015. Crepis foetida L. subsp rhoeadifolia (Bleb.) Celak. as a source of multifunctional agents: Cytotoxic and phytochemical evaluation. J Funct Foods; 17: 698-708.(doi:10.1016/j.jff.2015.06.041).
17. [17]. Tepe, B.; Sarikurkcu, C.; Berk, S.; Alim, A.; Akpulat, H. A. 2011. Chemical composition, radical scavenging and antimicrobial activity of the essential oils of Thymus boveii and Thymus hyemalis. Rec. Nat. Prod.; 5(3): 208-220.
18. [18]. Sabik, A. E.; Sevindik, M.; Mohammed, F. S.; Dogan, M. 2024. A New Natural Source against A549 Lung Cancer Cells Anthemis cotula and its Biological Activities and Phenolic Contents. Pharmaceutical Chemistry Journal; 58: 1134–1140. (doi:10.1007/s11094-024-03251-6).
19. [19]. Lo'ay, A.; Abu-Orabi, S. T.; Hlail, H. M.; Alkhatib, R. Q.; Al-Dalahmeh, Y.; Al-Qudah, M. A. 2023. Anthemis cotula L. from Jordan: Essential oil composition, LC-ESI-MS/MS profiling of phenolic acids-flavonoids and in vitro antioxidant activity. Arabian Journal of Chemistry; 16(2): 104470.(doi.org/10.1016/j.arabjc.2022.104470).
20. [20]. Al-Momani, A.; T Abu Orabi, S.; M Hlail, H.; Q Alkhatib, R.; Al-Dalahmeh, Y.; A Al-Qudah, M. 2022. Anthemis cotula L. from Jordan: Essential oil composition, LC-ESI-MS/MS profiling of phenolic acids-flavonoids and in vitro antioxidant activity. Arabian Journal of Chemistry; 16(2): 104470.(doi.org/10.1016/j.arabjc.2022.104470).