Investigation of Effective Immobilization Method for Ethanol Producing E. coli Strain

Yıl 2019, Cilt: 15 Sayı: 2, 217 - 220, 30.06.2019

Meltem Yesilcimen Akbas Taner Sar

https://doi.org/10.18466/cbayarfbe.528451

Cited By: 2

Öz

There is a growing interest to find alternative
cheap carbon sources for bioethanol production. Whey powder is a by-product of
cheese industry with high lactose content. Immobilization of bacteria is an
effective method for enhancing production of their metabolites including bioethanol.
In this work, a new and effective immobilization method for bioethanol
production from whey powder as a cheap/renewable carbon source was investigated.
For this, different immobilization methods were evaluated to avoid disruption
of bead structures during ethanol fermentation. The best results were obtained
when sodium alginate was sterilized under the UV lamp instead of heating for
immobilization, and the fermentation media was supplemented with CaCl₂
as a stability factor. In addition, the beads were successfully reused in at
least three 48 h batch fermentations for ethanol production

Anahtar Kelimeler

E.coli, ethanol, immobilization, repeated batch fermentation, whey powder, lactose

Kaynakça

• 1. Siso, MIG. 1996. The biotechnological utilization of cheese whey: A review. Bioresource Technology; 57: 1-11.
• 2. Das, B, Sarkar, S, Maiti, S, Bhattacharjee, S. 2016. Studies on production of ethanol from cheese whey using Kluyveromyces marxianus. Mater Today: Proceedings; 3: 3253-3257.
• 3. Ha, SJ, Galazka, JM, Kim, SR, Choi, JH, Yang, X, Seo JH, Glass NJ, Cate JHD, Jin YS. 2011. Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation. Proceedings of the National Academy of Sciences; 108(2): 504-509.
• 4. Zafar, S, Owais, M, Saleemuddin, M, Husain, S. 2005. Batch kinetics and modelling of ethanolic fermentation of whey. International Journal of Food Science & Technology; 40(6): 597-604.
• 5. Dien, BS, Nichols, NN, O’bryan, PJ, Bothast, RJ. 2000. Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosic biomass. Applied Microbiology and Biotechnology; 84-86: 181-196.
• 6. Sanny, T, Arnaldos, M, Kunkel, SA, Pagilla, KR, Stark, BC. 2010. Engineering of ethanolic E. coli with the Vitreoscilla hemoglobin gene enhances ethanol production from both glucose and xylose. Applied Microbiology and Biotechnology; 88: 1103-1112.
• 7. Abanoz, K., Stark, BC., Akbas, MY. 2012. Enhancement of ethanol production from potato‐processing wastewater by engineering Escherichia coli using Vitreoscilla haemoglobin. Letters in applied microbiology; 55(6): 436-443.
• 8. Akbas, MY, Sar T, Ozcelik B. 2014. Improved ethanol production from cheese whey, whey powder, and sugar beet molasses by “Vitreoscilla hemoglobin expressing” Escherichia coli. Bioscience, Biotechnology, and Biochemistry; 78(4): 687-694.
• 9. Sumer, F, Stark, BC, Akbas, MY. 2015. Efficient ethanol production from potato and corn processing industry waste using E. coli engineered to express Vitreoscilla haemoglobin. Environmental Technology; 36(18): 2319-2327.
• 10. Sar, T, Stark, BC, Akbas, MY. 2017a. Effective ethanol production from whey powder through immobilized E. coli expressing Vitreoscilla hemoglobin. Bioengineered; 8(2); 171-181.
• 11. Sar, T, Seker, G, Erman, AG, Stark, BC, Akbas, MY. 2017b. Repeated batch fermentation of immobilized E. coli expressing Vitreoscilla hemoglobin for long-term use. Bioengineered; 8(5): 651-660.
• 12. Akbas, MY, Stark, BC. 2016. Recent trends in bioethanol production from food processing byproducts. Journal of Industrial Microbiology & Biotechnology; 43(11): 1593-1609.
• 13. Razmovski, R, Vučurović, V. 2012. Bioethanol production from sugar beet molasses and thick juice using Saccharomyces cerevisiae immobilized on maize stem ground tissue. Fuel; 92: 1-8.
• 14. Karagoz, P, Ozkan, M. 2014. Ethanol production from wheat straw by Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture in batch and continuous system. Bioresource Technology; 158: 286-93.
• 15. Cheong, SH, Park, HK, Kim, BS, Chang, HN. 1993. Microencapsulation of yeast cells in the calcium alginate membrane. Biotechnology Techniques; 7: 879-84.
• 16. Chang, HN, Seong, GH, Yoo, IK, Park, JK, Seo, JH. 1996. Microencapsulation of recombinant Saccharomyces cerevisiae cells with invertase activity in liquid core alginate capsules. Biotechnology and Bioengineering; 51: 157-162.
• 17. Şar, T, Akbaş, MY. 2016. Biyoetanol üretimi için gıda işleme atıklarının asit hidrolizi. Academic Food Journal/Akademik GIDA; 14(1): 15-20.
• 18. Ozmihci, S, Kargi, F. 2007. Ethanol fermentation of cheese whey powder solution by repeated fed-batch operation. Enzyme and Microbial Technology, 41:169-74.
• 19. Singh, PK, Deol, PK, Kaur, IP. 2012. Entrapment of Lactobacillus acidophilus into alginate beads for the effective treatment of cold restraint stress induced gastric ulcer. Food & Function; 3(1): 83-90.
• 20. Azam, MM, Ezeji, TC, Qureshi, N. 2014. Novel technologies for enhanced production of ethanol: Impact of high productivity on process economics. European Chemical Bulletin, 3(9): 904-910.