Yıl 2019, Cilt 15 , Sayı 3, Sayfalar 265 - 270 2019-09-30

Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell

Tunahan Taşçi [1] , Vahap Eldem [2] , Melike Erkan [3]


Arsenic is a broad-spectrum environmental contaminant with mutagenic, teratogenic and carcinogenic effects. Due to its widespread distribution in nature, drinking water is the most common source of arsenic exposure for the general population. In this study, we aimed to determine the effect of sodium arsenite on the viability and expression profile of steroidogenic genes in TM3 Leydig cells, responsible for testicular steroidogenesis. The TM3 Leydig cells were treated with sodium arsenic (384,8 µM or 7,6 mM) for 24 hours with LH (Luteinizing hormone) stimulation. The MTT assay was used for measuring cell viability, the expression level of key genes of the steroidogenesis was evaluated using RT-qPCR.The MTT assay showed that cell viability was decreased dose-dependently. RT-qPCR demonstrated that the expression level of CYP11A1, CYP17A1 were decreased as compared to the untreated control while StAR gene expression was found to be surprisingly high in the cell exposed to high-dose arsenic (p<0.05). The expression profile of two dehydrogenase; 17β-HSD and 3β-HSD was significantly reduced in high dose arsenic treated-group (p<0.05); but however, no statistical significance was found in low-dose. The RT-qPCR also showed that the expression SF-1 (Steroidogenic factor-1), a key regulator of adrenal and reproductive development, was significantly decreased in both low-dose and high-dose (p<0.05). Arsenic toxicity in Leydig cell leads to cell viability loss and cause a perturbation in key steroidogenic genes, reflecting the possible role of arsenic in infertility and male reproductive system disorders.
Arsenic, Gene Expression, Leydig Cell, Steroidogenesis, Viability
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Birincil Dil en
Konular Mühendislik
Yayımlanma Tarihi 30-09-2019
Bölüm Makaleler
Yazarlar

Orcid: 0000-0002-2632-3516
Yazar: Tunahan Taşçi (Sorumlu Yazar)
Kurum: ISTANBUL BILGI UNIVERSITY
Ülke: Turkey


Orcid: 0000-0003-3767-5661
Yazar: Vahap Eldem
Kurum: İSTANBUL ÜNİVERSİTESİ
Ülke: Turkey


Yazar: Melike Erkan
Kurum: İSTANBUL ÜNİVERSİTESİ

Tarihler

Yayımlanma Tarihi : 30 Eylül 2019

Bibtex @araştırma makalesi { cbayarfbe540544, journal = {Celal Bayar University Journal of Science}, issn = {1305-130X}, eissn = {1305-1385}, address = {}, publisher = {Celal Bayar Üniversitesi}, year = {2019}, volume = {15}, pages = {265 - 270}, doi = {10.18466/cbayarfbe.540544}, title = {Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell}, key = {cite}, author = {Taşçi, Tunahan and Eldem, Vahap and Erkan, Melike} }
APA Taşçi, T , Eldem, V , Erkan, M . (2019). Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell. Celal Bayar University Journal of Science , 15 (3) , 265-270 . DOI: 10.18466/cbayarfbe.540544
MLA Taşçi, T , Eldem, V , Erkan, M . "Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell". Celal Bayar University Journal of Science 15 (2019 ): 265-270 <https://dergipark.org.tr/tr/pub/cbayarfbe/issue/48815/540544>
Chicago Taşçi, T , Eldem, V , Erkan, M . "Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell". Celal Bayar University Journal of Science 15 (2019 ): 265-270
RIS TY - JOUR T1 - Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell AU - Tunahan Taşçi , Vahap Eldem , Melike Erkan Y1 - 2019 PY - 2019 N1 - doi: 10.18466/cbayarfbe.540544 DO - 10.18466/cbayarfbe.540544 T2 - Celal Bayar University Journal of Science JF - Journal JO - JOR SP - 265 EP - 270 VL - 15 IS - 3 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.540544 UR - https://doi.org/10.18466/cbayarfbe.540544 Y2 - 2019 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell %A Tunahan Taşçi , Vahap Eldem , Melike Erkan %T Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell %D 2019 %J Celal Bayar University Journal of Science %P 1305-130X-1305-1385 %V 15 %N 3 %R doi: 10.18466/cbayarfbe.540544 %U 10.18466/cbayarfbe.540544
ISNAD Taşçi, Tunahan , Eldem, Vahap , Erkan, Melike . "Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell". Celal Bayar University Journal of Science 15 / 3 (Eylül 2019): 265-270 . https://doi.org/10.18466/cbayarfbe.540544
AMA Taşçi T , Eldem V , Erkan M . Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell. Celal Bayar Univ J Sci. 2019; 15(3): 265-270.
Vancouver Taşçi T , Eldem V , Erkan M . Sodium Arsenic Alters the Gene Expression of some Steroidogenic Genes in TM3 Leydig Cell. Celal Bayar University Journal of Science. 2019; 15(3): 270-265.