Yıl 2019, Cilt 15 , Sayı 3, Sayfalar 293 - 300 2019-09-30

Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study

Yücel Başımoğlu Koca [1]


In this study the effects of Diisobutyl phthalate (DIBP) from commonly used pytalates were histopathologically investigated in order to find out whether it is one of the causes of increased infertility in recent years. In addition, the localization of laminin and fibronectin was demonstrated immunohistochemically after exposure to DIBP. For this purpose, three study groups from Wistar albino rats were created: experimental group, control group and corn oil applied group. The experimental group were administered by gavage 3 different doses (0.25-0.5-1 ml/kg/day) of DIBP mixed with corn oil every day for 28 days. At the end of the experiment, the testis tissue samples from all of the experimental and control group animals were investigated and evaluated with a light microscope after routine histological and immunohistochemical preparation processes. Reduction in spermatogenic cell population, apoptotic and necrotic germ cells, increase in Sertoli cells, multinucleate giant cells, thickening and dilatation in basal lamina of seminiferous tubules, oedema and shrinkage in interstitial connective tissue were observed in DIBP treatment groups. The decrease in localization of fibronectin and laminin immunoreactivity in seminiferous tubules was observed. The results of this study demonstrated that DIBP causes serious damage to the testicular tissue, especially in terms of spermatogenesis. For this reason, it has come to the conclusion that the material in question may cause infertility in male individuals.
Diisobutyl phthalate, infertility, testes, laminin, fibronectin, histopathology
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Birincil Dil en
Konular Mühendislik
Yayımlanma Tarihi 30-09-2019
Bölüm Makaleler
Yazarlar

Yazar: Yücel Başımoğlu Koca (Sorumlu Yazar)
Kurum: AYDIN ADNAN MENDERES ÜNİVERSİTESİ
Ülke: Turkey


Destekleyen Kurum ADÜ/BAP
Proje Numarası FEF-2014/018
Teşekkür I thank the Adnan Menderes University for support of this research through grant number FEF-2014/018. Also, I thank Merve Akyıldız, master student, for the contribution of the laboratory work.
Tarihler

Yayımlanma Tarihi : 30 Eylül 2019

Bibtex @araştırma makalesi { cbayarfbe570613, journal = {Celal Bayar University Journal of Science}, issn = {1305-130X}, eissn = {1305-1385}, address = {}, publisher = {Celal Bayar Üniversitesi}, year = {2019}, volume = {15}, pages = {293 - 300}, doi = {10.18466/cbayarfbe.570613}, title = {Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study}, key = {cite}, author = {Başımoğlu Koca, Yücel} }
APA Başımoğlu Koca, Y . (2019). Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study. Celal Bayar University Journal of Science , 15 (3) , 293-300 . DOI: 10.18466/cbayarfbe.570613
MLA Başımoğlu Koca, Y . "Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study". Celal Bayar University Journal of Science 15 (2019 ): 293-300 <https://dergipark.org.tr/tr/pub/cbayarfbe/issue/48815/570613>
Chicago Başımoğlu Koca, Y . "Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study". Celal Bayar University Journal of Science 15 (2019 ): 293-300
RIS TY - JOUR T1 - Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study AU - Yücel Başımoğlu Koca Y1 - 2019 PY - 2019 N1 - doi: 10.18466/cbayarfbe.570613 DO - 10.18466/cbayarfbe.570613 T2 - Celal Bayar University Journal of Science JF - Journal JO - JOR SP - 293 EP - 300 VL - 15 IS - 3 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.570613 UR - https://doi.org/10.18466/cbayarfbe.570613 Y2 - 2019 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study %A Yücel Başımoğlu Koca %T Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study %D 2019 %J Celal Bayar University Journal of Science %P 1305-130X-1305-1385 %V 15 %N 3 %R doi: 10.18466/cbayarfbe.570613 %U 10.18466/cbayarfbe.570613
ISNAD Başımoğlu Koca, Yücel . "Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study". Celal Bayar University Journal of Science 15 / 3 (Eylül 2019): 293-300 . https://doi.org/10.18466/cbayarfbe.570613
AMA Başımoğlu Koca Y . Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study. Celal Bayar Univ J Sci. 2019; 15(3): 293-300.
Vancouver Başımoğlu Koca Y . Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study. Celal Bayar University Journal of Science. 2019; 15(3): 300-293.