Yıl 2020, Cilt 16 , Sayı 1, Sayfalar 9 - 14 2020-03-27

A Novel Biosensor Based on Laccase for The Detection of Catechol

Zehra ÖLÇER [1]


Phenolic compounds are broadly used in petrol industrial production processes and thus they are hazardous for ground and surface water and environment. Many of them have harmful effects on plants, animals and human health. Laccases can oxide phenol and phenolic compounds. Biosensors provide ideal sensing systems to determine phenolic compounds. In the present paper, it was developed novel sensor surface with modified laccase on gold electrode which could recognize the phenolic compound catechol. The electrode arrays integrated microfluidics have been fabricated and characterized in order to create a sensor chip which is easy, rapid, and cheaper to produce also have good electrochemical sensing properties. This gold electrodes were used to obtain laccase active surfaces. Laccase was covalently bounded to self-assembled monolayer on gold electrode via glutaraldehyde reactions. Cyclic voltammetry and amperometry activity assays showed that the detection of catechol with laccase modified gold surface. All of the study was carried out using automated biosensor device. Linear range for this biosensor was 0.025 mM to 0.8 mM.


Catechol, electrochemical biosensor, laccase immobilization
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Birincil Dil en
Bölüm Makaleler
Yazarlar

Orcid: 0000-0001-9747-2984
Yazar: Zehra ÖLÇER (Sorumlu Yazar)
Kurum: GEBZE TECHNICAL UNIVERSITY, FACULTY OF BASIC SCIENCES
Ülke: Turkey


Destekleyen Kurum Gebze Technical University
Proje Numarası BAP 2018-A105-23
Teşekkür The author would like to thanks the Gebze Technical University (GTU) for the provided financial support (Grant no: BAP 2018-A105-23). We gratefully acknowledge to Dr. Yıldız Uludag and Dr. Aylin Ersoy from BILGEM-TUBITAK for their contribution to the fabrication of the biochip and the sensing platform.
Tarihler

Yayımlanma Tarihi : 27 Mart 2020

Bibtex @araştırma makalesi { cbayarfbe634500, journal = {Celal Bayar University Journal of Science}, issn = {1305-130X}, eissn = {1305-1385}, address = {}, publisher = {Celal Bayar Üniversitesi}, year = {2020}, volume = {16}, pages = {9 - 14}, doi = {}, title = {A Novel Biosensor Based on Laccase for The Detection of Catechol}, key = {cite}, author = {ÖLÇER, Zehra} }
APA ÖLÇER, Z . (2020). A Novel Biosensor Based on Laccase for The Detection of Catechol. Celal Bayar University Journal of Science , 16 (1) , 9-14 . Retrieved from https://dergipark.org.tr/tr/pub/cbayarfbe/issue/53288/634500
MLA ÖLÇER, Z . "A Novel Biosensor Based on Laccase for The Detection of Catechol". Celal Bayar University Journal of Science 16 (2020 ): 9-14 <https://dergipark.org.tr/tr/pub/cbayarfbe/issue/53288/634500>
Chicago ÖLÇER, Z . "A Novel Biosensor Based on Laccase for The Detection of Catechol". Celal Bayar University Journal of Science 16 (2020 ): 9-14
RIS TY - JOUR T1 - A Novel Biosensor Based on Laccase for The Detection of Catechol AU - Zehra ÖLÇER Y1 - 2020 PY - 2020 N1 - DO - T2 - Celal Bayar University Journal of Science JF - Journal JO - JOR SP - 9 EP - 14 VL - 16 IS - 1 SN - 1305-130X-1305-1385 M3 - UR - Y2 - 2020 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi A Novel Biosensor Based on Laccase for The Detection of Catechol %A Zehra ÖLÇER %T A Novel Biosensor Based on Laccase for The Detection of Catechol %D 2020 %J Celal Bayar University Journal of Science %P 1305-130X-1305-1385 %V 16 %N 1 %R %U
ISNAD ÖLÇER, Zehra . "A Novel Biosensor Based on Laccase for The Detection of Catechol". Celal Bayar University Journal of Science 16 / 1 (Mart 2020): 9-14 .
AMA ÖLÇER Z . A Novel Biosensor Based on Laccase for The Detection of Catechol. Celal Bayar Univ J Sci. 2020; 16(1): 9-14.
Vancouver ÖLÇER Z . A Novel Biosensor Based on Laccase for The Detection of Catechol. Celal Bayar University Journal of Science. 2020; 16(1): 14-9.