Yıl 2020, Cilt 16 , Sayı 4, Sayfalar 403 - 407 2020-12-30

Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates

Ceren BASKAN [1]


In this study, a total of 68 raw milk samples were used to investigate the prevalence of Enterobacteriaceae in milk samples obtained from different dairy and supermarket in the province of Amasya (Turkey), as well as to determine the antibiotic resistance profile, the presence of Int1, blaTEM and blaSHV gene. In this study, isolates were obtained using classical culture technique. Then, detection of antibiotic resistance profile was carried out using disc diffusion methods. 12 different antibiotics were used as antibiotics including meropenem, cefotaxime, nalidixic acid, ceftriaxone, chloramphenicol, ceftazidime, streptomycin, ampicillin, gentamicin, tetracycline, levofloxacin and trimethoprim-sulfamethoxazole. Final, single strain PCR was created for the detection of ESBLs. For the aims, blaTEM and blaSHV genes (for determination of extended-spectrum beta-lactamase) were demonstrated by PCR assay. Then, for determination of Int1 was determined by using PCR assay. As a result, 50 isolates belonging to the Enterobacteiaceae family were obtained. Isolates against 41 (82%) ampicillin, 38 (76%) trimethoprim-sulfamethoxol, 7 (14%) ceftazidime, 6 (12%) cefotaxime, 2 (4%) meropenem and nalidixic acid and 1 (2%) ceftriaxone and streptomycin was determined as resistant. In addition, isolates were found to be 49 (98%), 50 (100%) 49 (98%) and 50 (100%) sensitive to chloramphenicol, gentamicin tetracycline and levofloxacin antibiotics, respectively. Among Enterobacteriaceae isolates, 22 (44%), 6 (12%) and 2 (4%) rates of strains were carrying Int1, blaSHV and blaTEM gene, respectively. In conclusion, the resistance of Enterobacteriaceae isolates isolated from milk samples to many antibiotics poses a potential danger in terms of public health
Enterobacteriaceae, antibiotic resistance, Int1, blaTEM, blaSHV, raw milk
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Birincil Dil en
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Orcid: 0000-0001-7849-4459
Yazar: Ceren BASKAN (Sorumlu Yazar)
Kurum: AMASYA ÜNİVERSİTESİ
Ülke: Turkey


Tarihler

Kabul Tarihi : 5 Aralık 2020
Yayımlanma Tarihi : 30 Aralık 2020

Bibtex @araştırma makalesi { cbayarfbe801544, journal = {Celal Bayar University Journal of Science}, issn = {1305-130X}, eissn = {1305-1385}, address = {}, publisher = {Celal Bayar Üniversitesi}, year = {2020}, volume = {16}, pages = {403 - 407}, doi = {}, title = {Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates}, key = {cite}, author = {Baskan, Ceren} }
APA Baskan, C . (2020). Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates . Celal Bayar University Journal of Science , 16 (4) , 403-407 . Retrieved from https://dergipark.org.tr/tr/pub/cbayarfbe/issue/58992/801544
MLA Baskan, C . "Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates" . Celal Bayar University Journal of Science 16 (2020 ): 403-407 <https://dergipark.org.tr/tr/pub/cbayarfbe/issue/58992/801544>
Chicago Baskan, C . "Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates". Celal Bayar University Journal of Science 16 (2020 ): 403-407
RIS TY - JOUR T1 - Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates AU - Ceren Baskan Y1 - 2020 PY - 2020 N1 - DO - T2 - Celal Bayar University Journal of Science JF - Journal JO - JOR SP - 403 EP - 407 VL - 16 IS - 4 SN - 1305-130X-1305-1385 M3 - UR - Y2 - 2020 ER -
EndNote %0 Celal Bayar Üniversitesi Fen Bilimleri Dergisi Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates %A Ceren Baskan %T Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates %D 2020 %J Celal Bayar University Journal of Science %P 1305-130X-1305-1385 %V 16 %N 4 %R %U
ISNAD Baskan, Ceren . "Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates". Celal Bayar University Journal of Science 16 / 4 (Aralık 2020): 403-407 .
AMA Baskan C . Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates. Celal Bayar Univ J Sci. 2020; 16(4): 403-407.
Vancouver Baskan C . Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates. Celal Bayar University Journal of Science. 2020; 16(4): 403-407.
IEEE C. Baskan , "Determination of Antibiotic Susceptibility Profile and Int1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates", Celal Bayar University Journal of Science, c. 16, sayı. 4, ss. 403-407, Ara. 2021