A Study on Total Phenolic and Flavonoid Content, Antioxidant, Toxicity, and Antihelmintic Activities of Methanol Extracts of Crocus cancellatus subsp. lycuis

Yıl 2023, Cilt: 19 Sayı: 1, 73 - 78, 28.03.2023

Mehlika Alper , Mehmet Özgür Atay , Olcay Ceylan , Ramazan Mammadov

https://doi.org/10.18466/cbayarfbe.1111317

Öz

The present study was planned to assess total phenolic and flavonoid content and some biological properties (antioxidant, toxicity, and antihelmintic) of the methanol extracts of Crocus cancellatus subsp. lycuis from Muğla province (Turkey) for the first time. Total phenolic (2.08±0.06 mg GAE/g) and total flavonoid (3.63±0.09 mg QE/g) contents of the aerial part extract were higher than the bulb extract. The aerial part extract (IC50 value: 0,86±0,03 mg/mL, IC50 value: 0,26±0,003 mg/mL, 0,77±0,007 mg TE/g, and 5,92±0,30 mg TE/g, respectively) was determined to exhibit the higher activity than that of bulb parts extract in the assays shortly named as DPPH, ABTS, FRAP and CUPRAC carried out to examine the antioxidant activities of the extracts. Both extracts were observed to be effective on brine shrimp and to have anthelmintic activity. According to these results, Crocus cancellatus subsp. lycuis may be paid attention as a good source for the pharmaceutical and food industries.

Anahtar Kelimeler

Anthelmintic , Antioxidant , Brine shrimp , Crocus , Flavonoid , Phenolic

Kaynakça

• 1]. Rehman, M. U., Wali, A. F., Ahmad, A., Shakeel, S., Rasool, S., Ali, R., ... & Khan, R. (2019). Neuroprotective strategies for neurological disorders by natural products: an update. Current Neuropharmacology,17(3),247-267.
• [2]. Bernardini, S., Tiezzi, A., Laghezza Masci, V., & Ovidi, E. (2018). Natural products for human health: an historical overview of the drug discovery approaches. Natural product research, 32(16), 1926-1950.
• [3]. Raina, H., Soni, G., Jauhari, N., Sharma, N., & Bharadvaja, N. (2014). Phytochemical importance of medicinal plants as potential sources of anticancer agents. Turkish Journal of Botany, 38(6), 1027-1035.
• [4]. Alamgir, A.N.M. (2018). Introduction. In: Therapeutic Use of Medicinal Plants and their Extracts: Volume 2. Progress in Drug Research, vol 74. Springer, Cham. https://doi.org/10.1007/978-3-319-92387-1_1
• [5]. Che, C. T., & Zhang, H. (2019). Plant Natural Products for Human Health. International journal of molecular sciences, 20(4), 830.https://doi.org/10.3390/ijms20040830
• [6]. Loizzo, O., Kovalyov, V., Goryacha, O., Ivanauskas, L., & Georgiyants, V. (2019). Biologically active compounds and pharmacological activities of species of the genus Crocus: A review. Phytochemistry,162,56-89.
• [7]. Mohtashami, L., Amiri, M. S., Ramezani, M., Emami, S. A., & Simal-Gandara, J. (2021). The genus Crocus L.: A review of ethnobotanical uses, phytochemistry and pharmacology. Industrial Cops and Products, 171, 113923.
• [8]. P.H. Davis, R. Mill, K. Tan Flora of Turkey and the East Aegean Islands (Supplement I), vol. 10, Edinburgh University Press, Edinburgh (1988) 278pp
• [9]. Verma, S. K., Das, A. K., Cingoz, G. S., Uslu, E., & Gurel, E. (2016). Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species. Biotechnology Reports, 10, 66-74.
• [10]. Turan, M., Mammadov, R. 2018. “Antioxidant, Antimicrobial, Cytotoxic, Larvicidal and Anthelmintic Activities and Phenolic Contents of Cyclamen alpinum”, Pharmacology & Pharmacy, 9, 100-116.
• [11]. Singleton, V.L., Rossi, J.A. 1965. ‘’Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents’’, American Journal of Enology and Viticulture, 16(3), 144-158.
• [12]. Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., Koirala, N. 2019. ‘’Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal’’, Plants, 8(4), 96.
• [13]. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. 1999. ‘’Antioxidant activity applying an improved ABTS radical cation decolorization assay’’, Free radical biology and medicine, 26(9-10), 1231-1237.
• [14]. Benzie, I. F., Strain, J. J. 1996. ‘’The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay’’, Analytical Biochemistry, 239(1), 70-76.
• [15]. Apak, R., Güçlü, K., Özyürek, M., Karademir, S. E. 2004. “Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method”, Journal of Agricultural and Food Chemistry, 52(26), 7970-7981.
• [16]. Krishnaraju, A. V., Rao, T. V., Sundararaju, D., Vanisree, M., Tsay, H. S., & Subbaraju, G. V. (2005). Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. International Journal of Applied Science and Engineering, 3(2), 125-134.
• [17]. Dutta et al. (2012). Dutta, B., Ghosal, M., Chakrabarty, P., & Mandal, P. (2012). Anthelmintic and free radicalscavenging potential of various fractions obtained from foliar parts of Glinus oppositifolius(Linn.) DC.International Journal of Pharmacy and Pharmaceutical Sciences,4(4), 233–239
• [18]. Cosme et al. 2020. Plant Phenolics: Bioavailability as a Key Determinant of Their Potential Health-Promoting Applications. Antioxidants 2020, 9, 1263; doi:10.3390/antiox9121263
• [19]. Loizzo et al. 2016. Crocus cancellatus subsp. damascenus stigmas: chemical profile, and inhibition of a-amylase, a-glucosidase and lipase, key enzymes related to type 2 diabetes and obesity. J Enzyme Inhib Med Chem, 2016; 31(2): 212–218.
• [20]. Karimi, E., Oskoueian, E., Hendra, R., & Jaafar, H. Z. (2010). Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity. Molecules,15(9),6244–6256.
• [21]. Satybaldiyeva et al. 2015. Satybaldiyeva, D., Mursaliyeva, V., Rakhimbayev, I., Zayadan, B., Mammadov, R., 2015. Preliminary phytochemical analysis and antioxidant, antibacterial activities of Crocus alatavicus from Kazakhstan. Not. Bot. Horti Agrobot. Cluj-Napoca 43, 343–348.
• [22]. Bellachioma, L., Rocchetti, G., Morresi, C., Martinelli, E., Lucini, L., Ferretti, G., ... & Bacchetti, T. (2022). Valorisation of Crocus sativus flower parts for herbal infusions: impact of brewing conditions on phenolic profiling, antioxidant capacity and sensory traits. International Journal of Food Science & Technology.
• [23]. Zengin et al. 2020. Chemical characterization, antioxidant, enzyme inhibitory and cytotoxic properties of two geophytes: Crocus pallasii and Cyclamen cilicium. Food Research International 133 (2020) 109129.
• [24]. Soobrattee et al., 2005. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. Volume 579, Issues 1–2, 11 November 2005, Pages 200-213.
• [25]. Lourenço, S. C., Moldão-Martins, M., & Alves, V. D. (2019). Antioxidants of Natural Plant Origins: From Sources to Food Industry Applications. Molecules (Basel, Switzerland), 24(22), 4132. https://doi.org/10.3390/molecules24224132.
• [26]. Ismael, 2021. Phytochemical screening and anti-candida activities of Crocus cancellatus herb. Ethanol extract. ZANCO Journal of Pure and Applied Sciences 33 (3): 124-133
• [27]. Khalili et al. 2016. Antioxidant activity of bulbs and aerial parts of Crocus caspius, impact of extraction methods. Pak. J. Pharm. Sci., 29(3), 2016, 773-777.
• [28]. Rahaiee, S., Moini, S., Hashemi, M. et al. Evaluation of antioxidant activities of bioactive compounds and various extracts obtained from saffron (Crocus sativus L.): a review. J Food Sci Technol 52, 1881–1888 (2015). https://doi.org/10.1007/s13197-013-1238-x
• [29]. Shakeri et al. 2019. Cytotoxic and Antioxidant Activities of Crocus pallasii subsp. haussknechtii Corms Extracts Compared with Crocus sativus. Research Journal of Pharmacognosy (RJP) 6(3), 2019: 51-59
• [30]. Sarah et al. 2017. Brine shrimp lethality assay. Bangladesh J Pharmacol. 2017; 12: 186-189.
• [31]. Mianabadi et al., 2015. Comparison of Cytotoxicity and anti-cancer activity, by Artemia urmiana Brine Shrimp Lethality Test(BSLT) and Cancer Cell leukemia and Breast Cancer by two species of Daphne
• [32]. Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E., McLaughlin, J.L., 1982. Brine Shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 45, 31-34.
• [33]. Peixoto E.C.T.M., Andrade A., Valadares F., Silva L.P. andSilva R.M.G. 2013. Phytoterapy in the control of helminthiasis in animal production. Afr. J. Agric. Res.8:2421-2429.
• [34]. Jayawardene, K. L. T., Palombo, E. A., & Boag, P. R. (2021). Natural Products Are a Promising Source for Anthelmintic Drug Discovery. Biomolecules, 11(10), 1457.