The Root Extracts of Valeriana Officinalis L. may Control Programmed Cell Death Pathways in Breast Cancer Cell Line, MCF-7

Öz

Natural product-derived phytochemicals are now accepted as promising agents in developing new strategies for cancer treatment and prevention. The root extracts of valerian (Valeriana officinalis L.), which is a supplement widely used for improving circadian rhythm-dependent sleep disorders and insomnia, might be a good candidate in that context. In the present study we hypothesized whether extract of valerian root induce programmed cell death machineries in a human breast cancer model, MCF-7 cells. To test the hypothesis, we treated MCF-7 cells with the extract at different concentrations for 24 h. Giemsa staining was used to evaluate the apoptotic morphology and apoptotic index, and monodancylcadaverine assay was used to determine vacuoles that are associated with autophagic flux. Our results indicated that extracts of the roots of valerian have apototic and autophagic effect on human breast cancer cell MCF-7 in a dose dependent manner. Moreover, the typical cobblestone morphology of the cells was disrupted after the extract treatments and the cells lost contact with each other. This morphological alteration was attributed to anoikis, is a programmed cell death type induced by loss of cell communication with extracellular matrix or neighboring cells. In conclusion, although this study has many methodological shortcomings, our findings suggest that Valeriana officinalis L. might be a potential anti-cancer agent for the treatment of breast cancer.

Anahtar Kelimeler

• Phytotherapy
• Valeriana officinalis L.
• Breast cancer
• Apoptosis
• Autophagy
• Anoikis

Valeriana Officinalis L. Kök Ekstraktları Meme Kanseri Hücre Hattı MCF-7'de Programlanmış Hücre Ölümü Yolaklarını Kontrol Edebilir

Öz

Günümüzde doğal ürünlerden elde edilen fitokimyasallar kanserin tedavisi ve önlenmesi için yeni stratejiler geliştirmede umut verici ajanlar olarak kabul edilmektedir. Sirkadiyen ritme bağlı uyku bozukluklarını ve uykusuzluğu tedavi etmek için yaygın olarak kullanılan bir takviye olan kediotu (Valeriana officinalis L.) kök ekstreleri bu bağlamda iyi bir aday olabilir. Bu çalışmada, kediotu kökü ekstraktının bir insan meme kanseri modeli olan MCF-7 hücrelerinde programlanmış hücre ölüm mekanizmalarını indükleyip indüklemediği hipotezini ortaya koyduk. Hipotezi test etmek için MCF-7 hücrelerini 24 saat boyunca farklı konsantrasyonlarda ekstrakt ile muamele ettik. Apoptotik morfolojiyi ve apoptotik indeksi değerlendirmek için Giemsa boyamasını, otofajik akı ile ilişkili vakuolleri belirlemek için ise monodansilkadaverin testini kullandık. Bulgularımız, kediotu köklerinden elde edilen ekstraktların insan meme kanseri hücresi MCF-7 üzerinde doza bağlı bir şekilde apototik ve otofajik etkiye sahip olduğunu göstermiştir. Ayrıca, hücrelerin tipik parke taşı morfolojisi ekstrakt uygulamalarından sonra bozulmuş ve hücreler birbirleriyle temasını kaybetmiştir. Bu morfolojik değişiklik, ekstrasellüler matriks veya komşu hücrelerle hücre iletişiminin kaybı ile indüklenen programlanmış bir hücre ölümü tipi olan anoikise atfedilmiştir. Sonuç olarak, bu çalışmada birçok metodolojik eksiklik olmasına rağmen, bulgularımız Valeriana officinalis L.'nin meme kanseri tedavisinde potansiyel bir anti-kanser ajan olabileceğini düşündürmektedir.

Anahtar Kelimeler

• Fitoterapi
• Valeriana officinalis L..
• Meme kanseri
• Apoptosis
• Otofaji
• Anoikis

Kaynakça

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