İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu

Onur Uysal; Tuğba Semerci Sevimli; Sibel Güneş; Ahmad Fahim Tokhi; Ceren Özel; Ayla Eker Sarıboyacı

doi:10.34087/cbusbed.616566

İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu

Abstract

Amaç: Mezenkimal kök hücreler (MKH), rejeneratif, immunosupresif, non-immunojenik, anjiyojenik, antiapoptotik ve antiinflamatuvar özellikleri sayesinde hem preklinik araştırmalarda hem de kilinikte önem taşımaktadır. MKH’lerin immunosupresif fonksiyonlarının keşfi, otoimmün hastalıklarda patojenik immun yanıtı düşürmek için yeni terapötik araç olarak araştırmalara konu olmuştur. Plasenta, göbek kordonu ve amniyon sıvısı; kemik iliği ve yağ kökenli MKH’lere göre non-invazif eldesi ve pluripotensi kapasitesiyle avantajlı kök hücre kaynakları arasındadır. Bu çalışmada immün yanıtların incelenmesi için in vitro bir model kullanılarak insan amniyotik sıvı kökenli MKH’lerin (iAS-MKH), fitohemaglütinin ile aktive edilmiş T-hücreleri (PHA-T hücreleri) üzerindeki etkisini; hücre proliferasyonu, apoptoz, sitokin düzeyleri ve regülatuar T hücre (Treg) farklılaşması açısından analiz edilmesi amaçlandı.

Gereç ve yöntem: Periferik kandan immunoseleksiyon yöntemi ile izole edilen T-hücreleri karakterize edilip PHA ile uyarıldıktan sonra, iAS-MKH’ler ile insertler kullanılarak indirekt yolla 4 gün ko-kültür (transwell) edildi. Kültür sonrasında PHA-T-hücreleri flow sitometri ve ELISA ile hücre canlılığı ve proliferasyon, sitokin düzeyleri, Treg-farklılaşması ve apoptotik açıdan analiz edildi.

Bulgular: iAS-MKH'lerin; in vitroda ko-kültür edildikleri PHA-T hücrelerinin sitokin profilini değiştirerek, canlılık ve proliferasyonu azaltarak ve apoptoz ve Treg farklılaşmasını indükleyerek immunosupresif etki gösterdiği gözlendi.

Sonuç: Erken dönemde (prenatal-natal) ve non-invazif tekniklerle eldesi ve atık bir doku olması şeklinde avantajları olan amniyon sıvısındaki bu kök hücrelerin, immunosupresif etkileri olduğu ve ileriye yönelik olarak daha ayrıntılı analizlerle ve in vivo çalışmalarla desteklenmesi gerektiği sonucuna varıldı.

Keywords

insan amniyon sıvısı,mezenkimal kök hücre,immunosupresyon

Supporting Institution

Eskişehir osmangazi Üniversitesi

Project Number

2018-46D16

Thanks

Bu çalışmada bizi destekleyen Eskişehir Osmangazi Üniversitesi Bilimsel Araştırma Projeleri Komisyonu’na (Proje No: 2018-46D16) sonsuz teşekkürlerimizi sunarız.

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Details

Primary Language

Turkish

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Onur Uysal ^*
0000-0001-6800-5607
Türkiye

Tuğba Semerci Sevimli
0000-0003-4856-2304
Türkiye

Sibel Güneş This is me
0000-0003-0202-5052
Türkiye

Ahmad Fahim Tokhi This is me
0000-0001-8955-2394
Türkiye

Ceren Özel This is me
0000-0002-5648-3174
Türkiye

Ayla Eker Sarıboyacı
0000-0003-4536-9859
Türkiye

Publication Date

December 31, 2019

Submission Date

September 6, 2019

Acceptance Date

December 25, 2019

Published in Issue

Year 2019 Volume: 6 Number: 4

DOI

https://doi.org/10.34087/cbusbed.616566

IZ

https://izlik.org/JA55TK83JA

Cite

RIS / Bibtex

APA

Uysal, O., Semerci Sevimli, T., Güneş, S., Tokhi, A. F., Özel, C., & Eker Sarıboyacı, A. (2019). İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal, 6(4), 313-321. https://doi.org/10.34087/cbusbed.616566

AMA

1.Uysal O, Semerci Sevimli T, Güneş S, Tokhi AF, Özel C, Eker Sarıboyacı A. İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. 2019;6(4):313-321. doi:10.34087/cbusbed.616566

Chicago

Uysal, Onur, Tuğba Semerci Sevimli, Sibel Güneş, Ahmad Fahim Tokhi, Ceren Özel, and Ayla Eker Sarıboyacı. 2019. “İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu”. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal 6 (4): 313-21. https://doi.org/10.34087/cbusbed.616566.

EndNote

Uysal O, Semerci Sevimli T, Güneş S, Tokhi AF, Özel C, Eker Sarıboyacı A (December 1, 2019) İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal 6 4 313–321.

IEEE

[1]O. Uysal, T. Semerci Sevimli, S. Güneş, A. F. Tokhi, C. Özel, and A. Eker Sarıboyacı, “İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu”, CBU-SBED: Celal Bayar University-Health Sciences Institute Journal, vol. 6, no. 4, pp. 313–321, Dec. 2019, doi: 10.34087/cbusbed.616566.

ISNAD

Uysal, Onur - Semerci Sevimli, Tuğba - Güneş, Sibel - Tokhi, Ahmad Fahim - Özel, Ceren - Eker Sarıboyacı, Ayla. “İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu”. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal 6/4 (December 1, 2019): 313-321. https://doi.org/10.34087/cbusbed.616566.

JAMA

1.Uysal O, Semerci Sevimli T, Güneş S, Tokhi AF, Özel C, Eker Sarıboyacı A. İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. 2019;6:313–321.

MLA

Uysal, Onur, et al. “İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu”. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal, vol. 6, no. 4, Dec. 2019, pp. 313-21, doi:10.34087/cbusbed.616566.

Vancouver

1.Onur Uysal, Tuğba Semerci Sevimli, Sibel Güneş, Ahmad Fahim Tokhi, Ceren Özel, Ayla Eker Sarıboyacı. İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. 2019 Dec. 1;6(4):313-21. doi:10.34087/cbusbed.616566