İnsan Amniyon Sıvısı Kökenli Mezenkimal Kök Hücrelerin İmmunosupresyon Etkileri: Treg Regülasyonu

Yıl 2019, , 313 - 321, 31.12.2019

Onur Uysal , Tuğba Semerci Sevimli , Sibel Güneş Ahmad Fahim Tokhi Ceren Özel Ayla Eker Sarıboyacı

https://doi.org/10.34087/cbusbed.616566

Öz

Amaç: Mezenkimal kök hücreler (MKH),
rejeneratif, immunosupresif, non-immunojenik, anjiyojenik, antiapoptotik ve
antiinflamatuvar özellikleri sayesinde hem preklinik araştırmalarda hem de
kilinikte önem taşımaktadır. MKH’lerin immunosupresif fonksiyonlarının keşfi,
otoimmün hastalıklarda patojenik immun yanıtı düşürmek için yeni terapötik araç
olarak araştırmalara konu olmuştur. Plasenta, göbek kordonu ve amniyon sıvısı;
kemik iliği ve yağ kökenli MKH’lere göre non-invazif eldesi ve pluripotensi
kapasitesiyle avantajlı kök hücre kaynakları arasındadır. Bu çalışmada immün
yanıtların incelenmesi için in vitro bir model kullanılarak insan amniyotik
sıvı kökenli MKH’lerin (iAS-MKH), fitohemaglütinin ile aktive edilmiş
T-hücreleri (PHA-T hücreleri) üzerindeki etkisini; hücre proliferasyonu,
apoptoz, sitokin düzeyleri ve regülatuar T hücre (Treg) farklılaşması açısından
analiz edilmesi amaçlandı.

Gereç ve yöntem: Periferik kandan immunoseleksiyon
yöntemi ile izole edilen T-hücreleri karakterize edilip PHA ile uyarıldıktan
sonra, iAS-MKH’ler ile insertler kullanılarak indirekt yolla 4 gün ko-kültür
(transwell) edildi. Kültür sonrasında PHA-T-hücreleri flow sitometri ve ELISA
ile hücre canlılığı ve proliferasyon, sitokin düzeyleri, Treg-farklılaşması ve
apoptotik açıdan analiz edildi.

Bulgular: iAS-MKH'lerin; in vitroda ko-kültür
edildikleri PHA-T hücrelerinin sitokin profilini değiştirerek, canlılık ve
proliferasyonu azaltarak ve apoptoz ve Treg farklılaşmasını indükleyerek
immunosupresif etki gösterdiği gözlendi.

Sonuç: Erken dönemde (prenatal-natal) ve
non-invazif tekniklerle eldesi ve atık bir doku olması şeklinde avantajları
olan amniyon sıvısındaki bu kök hücrelerin, immunosupresif etkileri olduğu ve
ileriye yönelik olarak daha ayrıntılı analizlerle ve in vivo çalışmalarla desteklenmesi
gerektiği sonucuna varıldı.

Anahtar Kelimeler

insan amniyon sıvısı, mezenkimal kök hücre, immunosupresyon

Destekleyen Kurum

Eskişehir osmangazi Üniversitesi

Proje Numarası

2018-46D16

Teşekkür

Bu çalışmada bizi destekleyen Eskişehir Osmangazi Üniversitesi Bilimsel Araştırma Projeleri Komisyonu’na (Proje No: 2018-46D16) sonsuz teşekkürlerimizi sunarız.

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