Çeşitli Anestezik İlaçların Antimikrobiyal ve Antioksidatif Özelliklerinin Belirlenmesi

Öz

Genel anestezi sırasında çeşitli nedenlerle oksidatif stres ile antioksidan savunma sistemi arasındaki denge bozulur. Öte yandan, antioksidan etkileri sayesinde bazı anestetiklerin patolojik durumların neden olduğu oksidatif stresten korunmaları önerilmiştir. Bu çalışmada ameliyat sonrası olası etkileri ortaya çıkarmak için yaygın olarak kullanılan anestezik ilaçların potansiyel antimikrobiyal ve antioksidatif aktiviteleri değerlendirildi. Kirby-Bauer disk difüzyon yöntemine göre steril fizyolojik tuzlu su ile seyreltilmiş ticari olarak satın alınan anestezik ilaçların antimikrobiyal aktiviteleri araştırıldı. Ayrıca minimum inhibitör konsantrasyon ve minimum bakterisidal konsantrasyonlar belirlendi. İlaçların antioksidan potansiyelleri 2,2 difenil-1-pikrilhidrazil (DPPH) ve hidroksil (OH●) radikal süpürücü yöntemleri ile tarandı. Ana bileşen olarak ketamin hidroklorür içeren test edilen ilaçlardan biri olan Ketalar’ın, test edilen tüm patojenik mikroorganizmalar üzerinde inhibisyon etkisi olduğu bulundu. Aynı zamanda, Mivacron ve Pental Sodium, Micrococcus luteus üzerinde en büyük inhibisyon bölgelerini oluşturmuştur. Beklendiği gibi, Propofol, test edilen organizmaların çoğunda antimikrobiyal aktivite göstermemiş olup, antioksidan aktivitesi diğer ilaçlar arasında en yüksek değere sahiptir. Ultiva, Blok-L ve Zolamid'in DPPH serbest radikali ve hidroksil radikal süpürücü aktiviteleri için hesaplanan SC50 değerleri Propofol'e çok yakın olduğu belirlendi. Bu ilaçların birçok durumda anestezi amaçlı kullanımı sırasında oluşabilecek kontaminasyon ve oksidatif stres riskini baskılayabileceği sonucuna varılabilir.

Anahtar Kelimeler

• DPPH
• Kirby-Bauer disk difüzyon metodu
• MİK
• Propofol

Determination of Antimicrobial and Antioxidative Properties of Several Anesthetic Drugs

Öz

For various reasons, the balance between oxidative stress and the antioxidative defence system is disturbed during general anesthesia. On the other hand, thanks to their antioxidant effect, certain anesthetics have been suggested to protect from oxidative stress caused due to pathological states. In this study, potential antimicrobial and antioxidative activities of commonly used anesthetic drugs were evaluated to reveal possible effects after surgery. The antimicrobial activities of commercially purchased anesthetic drugs diluted with sterile physiological saline were investigated according to the Kirby-Bauer disc diffusion method. Furthermore, minimum inhibitory concentration and minimum bactericidal concentrations were determined. Antioxidative potentials of the drugs were screened according to 2,2 diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH●) radical scavenging assays. One of the tested drugs, Ketalar, containing ketamine hydrochloride was found to have an inhibition effect on all tested pathogenic microorganisms. At the same time, Mivacron and Pental Sodium formed the most significant inhibition zones on Micrococcus luteus. As expected, Propofol had no antimicrobial activity on most tested organisms. However, its antioxidant activity was the highest among the other drugs. Calculated SC50 values for DPPH-free and hydroxyl radical scavenging activities of Ultiva, Blok-L, and Zolamid were very close to Propofol. It can be concluded that using these drugs for anesthesia may suppress the risk of contamination and oxidative stress that may occur during use in many cases.

Anahtar Kelimeler

• DPPH
• Kirby-Bauer disc diffusion method
• MIC
• Propofol

Kaynakça

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