Araştırma Makalesi
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Effect of Iron and Zinc Applications on the Growth and Development of Soybeans [Glycine max (L.) Merr.]

Yıl 2025, Cilt: 9 Sayı: 2, 220 - 226, 18.12.2025
https://doi.org/10.31594/commagene.1802380

Öz

This study was conducted to determine the effects of iron (Fe) and zinc (Zn) on the growth and development of soybeans (Glycine max (L.) Merr.). Healthy and germination-suitable soybean seeds were germinated in magentas with distilled water 25±2 °C. The germinated seeds were then transplanted into perlite pots and grown in a climate chamber. During the experimental phase, iron and zinc were applied separately in five groups: 0% (control), 20%, 30%, 40%, and 50%. Seedlings grown for 28 days were harvested and leaf samples from each pot were stored in a freezer for analysis. Proline, chlorophyll, malondialdehyde (MDA), and ion (Na⁺, K⁺, Ca²⁺, Cl⁻) analyses were performed on the leaf samples. Significant increases were observed in proline, chlorophyll, and MDA compared to the control group. The study concluded that a 40% dose of iron and zinc increased resistance in soybean plants and positively affected their growth and development depending on the applied ratios.

Kaynakça

  • Anonim, (2019). Karma yem sanayi raporu. Türkiye Yem Sanayicileri Birliği (TÜKİYEMBİR), Poyraz Ofset Matbaacılık, Ankara, 64pp.
  • Arıoğlu, H., Bakal, H., & Güllüoğlu, L. (2012). Tohumluk soya üretiminde hasat öncesi yağan yağmurların tohum kalitesi üzerine etkileri. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 27(2), 29–36.
  • Aydeniz, A., & Brohi, A.R. (1991). Gübreler ve gübreleme. Cumhuriyet Üniversitesi Tokat Ziraat Fakültesi Yayın No 10, Tokat, 271pp.
  • Bates, L.S., Waldren, R.P., & Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205–207.
  • Ben-Amor, N., Jimenez, A., Megdiche, W., Lundqvist, M., Sevilla, F., & Abdelly, C. (2006). Response of antioxidant systems to NaCl stress in the halophyte Cakile maritima. Physiologia Plantarum, 126, 446–457.
  • Boşgelmez, A., Boşgelmez, İ., Savaşçı, S., & Paslı, N. (2001). Ekoloji II (Toprak). Başkent Klişe Matbaacılık, Ankara, 872 pp.
  • Brady, N.C. (1990). The nature and properties of soils (10th ed.). Macmillan Publishing Company, New Yor, 621 pp.
  • Dalcorso, G., Fasani, E., & Furini, A. (2013). Recent advances in the analysis of metal hyperaccumulation and hypertolerance in plants using proteomics. Frontiers in Plant Science, 4, 280.
  • Dede, Ö., & Acar, İ. (2024). Effects of foliar and soil applied zinc on yield and its components in soybean (Glycine max L. Merr.). Akademik Ziraat Dergisi, 13(2), 362–368.
  • Demirevska, K., Simova-Stoilova, L., Fedina, I., Georgieva, K., & Kunert, K. (2010). Response of oryzacystatin I transformed tobacco plants to drought, heat and light stress. Journal of Agronomy and Crop Science, 196, 90–99.
  • Demirkıran, A.R., & Cengiz, M.C. (2010). Effect of different organic material and chemical fertilizers on nutrition of pistachio (Pistacia vera L.) in organic arboriculture. International Journal of Physical Sciences, 5(10), 1600–1606.
  • Doğan, M., & Avu, A. (2013). Kuraklık stresine karşı bor bileşiklerinin soyada (Glycine max L.) büyüme parametrelerine etkisi. Nevşehir Bilim ve Teknoloji Dergisi, 2(2), 1–13.
  • Doğan, Y. (2015). Investigation of micro and macro element content of wheat varieties grown commonly in Turkey. Oxidation Communications, 38(3), 1265–1272.
  • Ellis, R.H., Roberts, E.H., Summerfield, R.J., & Cooper, J.P. (1988). Environmental control of flowering in barley (Hordeum vulgare L.): II. Rate of development as a function of temperature and photoperiod and its modification by low temperature vernalization. Annals of Botany, 62, 145–158.
  • Eren, A. (2018). Bakırla kirlenmiş toprakların Xanthium strumarium L. bitkisi kullanılarak fitoremediasyonu. Türkiye Tarımsal Araştırmalar Dergisi, 5(2), 152–157.
  • Hoagland, D.R., & Arnon, D.I. (1938). The water culture method for growing plants without soil. California Agricultural Experiment Station Circular, 347, 1–32.
  • Islam, S., Malik, A.I., Islam, A.K.M.R., & Colmer, T.D. (2007). Salt tolerance in a Hordeum marinum–Triticum aestivum amphiploid and its parents. Journal of Experimental Botany, 58, 1219–1229.
  • Kaçar, B., & Katkat, V. (2023). Bitki besleme (5. baskı). Nobel Akademik Yayıncılık, Ankara, 678 pp.
  • Kumar, S.S., Kadier, A., Malyan, S.K., Ahmad, A., & Bishnoi, N.R. (2017). Phytoremediation and rhizoremediation: Uptake, mobilization and sequestration of heavy metals by plants. In Singh, D., Singh, H., Prabha, R. (eds): Plant–microbe interactions in agro-ecological perspectives. Springer, Singapore, 367–394.
  • Kumar, S.A., Kumar, A., Bhargava, C.R., Patel, R., Dwarka, D., Singh, O., Sharma, S., Jain, S.K., Jain, D., & Ahirwar, M.K. (2023). The effect of various doses of zinc on the growth and yield of soybean crop (Glycine max L.). International Journal of Plant & Soil Science, 35(24), 308–312.
  • Larcher, W. (2003). Physiological plant ecology. Berlin, Springer-Verlag. 107pp.
  • Luna, C., Seffino, L.G., Arias, C., & Taleisnik, E. (2000). Oxidative stress indicators as selection tools for salt tolerance in Chloris gayana. Plant Breeding, 119, 341–345.
  • Lutts, S., Kinet, J.M., & Bouharmont, J. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany, 78, 389–398.
  • Maes, W.H., Achten, W.M.J., Reubens, B., Raes, D., Samson, R., & Muys, B. (2009). Plant–water relationships and growth strategies of Jatropha curcas L. seedlings under different levels of drought stress. Journal of Arid Environments, 73, 877–884.
  • McCauley, A., Jones, C., & Jacobsen, J. (2009). Nutrient management module No. 9. S, 16, Montana State University Extension Service. Publication, Bozeman, USA.
  • Neto, A.D.A., Prisco, J.T., Eneas-Filho, J., Abreu, C.E.B., & Gomez-Filho, E. (2005). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Journal of Experimental Botany, 56, 87–94.
  • Özer, İ. (2025). Effects of foliar application of iron (Fe) and zinc (Zn) on agronomic characteristics of soybean (Glycine max L.). International Journal of Environmental Trends, 9(1), 48–55.
  • Özüstün, A. (2022). Farklı ekolojik koşullarda soya fasulyesi (Glycine max (L.) Merr.) çeşitlerinin verim ve kalite özelliklerinin belirlenmesi (742590). Retrieved from https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
  • Parvaiz, A., & Satyawati, S. (2008). Salt stress and phytochemical responses of plants – a review. Plant, Soil and Environment, 54, 89–99.
  • Ribeiro, R.V., Machado, E.C., Santos, M.G., & Oliveira, R.F. (2009). Photosynthesis and water relations of well-watered orange plants as affected by winter and summer conditions. Photosynthetica, 47, 215–222.
  • Sharma, P., & Dietz, K.-J. (2009). The relationship between metal toxicity and oxidative stress in plants. Journal of Plant Physiology, 166(10), 1132–1141.
  • Sharma, R., Bhardwaj, R., Thukral, A.K., Al-Huqail, A.A., Siddiqui, M.H., & Ahmad, P. (2019). Oxidative stress mitigation and initiation of antioxidant and osmoprotectant responses mediated by ascorbic acid in Brassica juncea L. subjected to copper (II) stress. Ecotoxicology and Environmental Safety, 182, 109436.
  • Taleisnik, E., Peyrano, G., & Arias, C. (1997). Response of Chloris gayana cultivars to salinity. I. Germination and early vegetative growth. Tropical Grasslands, 31, 232–240.
  • Turfan, N. (2022). Bitkilerde ağır metal stresi. In: Seçkin,Dinler, B.&Gül, V. (Ed.) Bitkilerde abiyotik ve biyotik stres yönetimi. Ankara, İKSAD Publishing House, 165–192.
  • Tunçtürk, R., Kulaz, H., & Tunçtürk, M. (2016). Effect of humic acid applications on some nutrient contents of soybean (Glycine max L.) cultivars. Communications, 39(1-II), 503–510.
  • Ünsal, H. (2007). Alkalin topraklarda humik asit ve çinko uygulamalarının iki farklı nohut çeşidinde verim ve N, P, K içeriğine etkisi (213618). Retrieved from https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
  • Vernay, P., Gauthier-Moussard, C., Jean, L., Bordas, F., Faure, O., Ledoigt, G., & Hitmi, A. (2008). Effect of chromium species on phytochemical and physiological parameters in Datura innoxia. Chemosphere, 72(5), 763–771.
  • Verbruggen, N., & Hermans, C. (2008). Proline accumulation in plants: A review. Amino Acids, 35, 753–759.
  • Walia, H., Wilson, C., Condamine, P., Liu, X., Abdelbagi, M.I., Zeng, L., Mandal, J., Xu, J., Cui, X., & Timothy, C.J. (2005). Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage. Plant Physiology, 139, 833–835.
  • Wolterbeek, H.T., & De Bruin, M. (1986). Xylem and phloem import of Na+, K+, Rb+, Cs+ and Sb(SO4)2 in tomato fruits: Differential contributions from stem and leaf. Journal of Experimental Botany, 37, 928–939.
  • Yiğit, A., Erekul, O., & Yaraşır, N. (2021). Kısıtlı Sulama ve Kükürt Dozu Uygulamalarının Soya Fasulyesinde Bitki Gelişimi ve Klorofil (SPAD) İçeriğine Etkisi. ÇOMÜ Ziraat Fakültesi Dergisi, 9(1), 105-117.

Demir ve Çinko Uygulamalarının Soya [Glycine max (L.) Merr.] Bitkisinin Büyüme ve Gelişimine Etkisi

Yıl 2025, Cilt: 9 Sayı: 2, 220 - 226, 18.12.2025
https://doi.org/10.31594/commagene.1802380

Öz

Bu araştırmayla, demir (Fe) ile çinkonun (Zn), soya (Glycine max (L.) Merr.) bitkisinin büyüme ve gelişimine etkisi amaçlanmıştır. Sağlam ve çimlenmeye uygun soya tohumları mağentalarda 25±2 °C sıcaklıkta saf su ile çimlendirilmiş, ardından perlit ortamında saksılara alınarak iklim odasında büyütülmüştür. Deneme aşamasında bitkilere demir ve çinko uygulamaları ayrı ayrı %0 (kontrol), %20, %30, %40 ve %50 oranlarında beş grupta yapılmıştır. 28 gün büyütülen fideler hasat edilerek her saksıdan alınan yaprak örnekleri dondurucuda saklanmış ve yapraklarda prolin, klorofil, malondialdehid (MDA) ve iyon (Na⁺, K⁺, Ca²⁺, Cl⁻) analizleri gerçekleştirilmiştir. Özellikle prolin, klorofil ve MDA analizlerinde kontrol grubuna göre anlamlı artışlar saptanmıştır. Araştırma sonucunda %40 oranında demir ve çinkonun, soya bitkisinin direnç mekanizmalarını güçlendirdiği ve büyüme gelişimini olumlu yönde etkilediği anlaşılmıştır.

Etik Beyan

ETİK RAPORU GEREKMİYOR

Kaynakça

  • Anonim, (2019). Karma yem sanayi raporu. Türkiye Yem Sanayicileri Birliği (TÜKİYEMBİR), Poyraz Ofset Matbaacılık, Ankara, 64pp.
  • Arıoğlu, H., Bakal, H., & Güllüoğlu, L. (2012). Tohumluk soya üretiminde hasat öncesi yağan yağmurların tohum kalitesi üzerine etkileri. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 27(2), 29–36.
  • Aydeniz, A., & Brohi, A.R. (1991). Gübreler ve gübreleme. Cumhuriyet Üniversitesi Tokat Ziraat Fakültesi Yayın No 10, Tokat, 271pp.
  • Bates, L.S., Waldren, R.P., & Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205–207.
  • Ben-Amor, N., Jimenez, A., Megdiche, W., Lundqvist, M., Sevilla, F., & Abdelly, C. (2006). Response of antioxidant systems to NaCl stress in the halophyte Cakile maritima. Physiologia Plantarum, 126, 446–457.
  • Boşgelmez, A., Boşgelmez, İ., Savaşçı, S., & Paslı, N. (2001). Ekoloji II (Toprak). Başkent Klişe Matbaacılık, Ankara, 872 pp.
  • Brady, N.C. (1990). The nature and properties of soils (10th ed.). Macmillan Publishing Company, New Yor, 621 pp.
  • Dalcorso, G., Fasani, E., & Furini, A. (2013). Recent advances in the analysis of metal hyperaccumulation and hypertolerance in plants using proteomics. Frontiers in Plant Science, 4, 280.
  • Dede, Ö., & Acar, İ. (2024). Effects of foliar and soil applied zinc on yield and its components in soybean (Glycine max L. Merr.). Akademik Ziraat Dergisi, 13(2), 362–368.
  • Demirevska, K., Simova-Stoilova, L., Fedina, I., Georgieva, K., & Kunert, K. (2010). Response of oryzacystatin I transformed tobacco plants to drought, heat and light stress. Journal of Agronomy and Crop Science, 196, 90–99.
  • Demirkıran, A.R., & Cengiz, M.C. (2010). Effect of different organic material and chemical fertilizers on nutrition of pistachio (Pistacia vera L.) in organic arboriculture. International Journal of Physical Sciences, 5(10), 1600–1606.
  • Doğan, M., & Avu, A. (2013). Kuraklık stresine karşı bor bileşiklerinin soyada (Glycine max L.) büyüme parametrelerine etkisi. Nevşehir Bilim ve Teknoloji Dergisi, 2(2), 1–13.
  • Doğan, Y. (2015). Investigation of micro and macro element content of wheat varieties grown commonly in Turkey. Oxidation Communications, 38(3), 1265–1272.
  • Ellis, R.H., Roberts, E.H., Summerfield, R.J., & Cooper, J.P. (1988). Environmental control of flowering in barley (Hordeum vulgare L.): II. Rate of development as a function of temperature and photoperiod and its modification by low temperature vernalization. Annals of Botany, 62, 145–158.
  • Eren, A. (2018). Bakırla kirlenmiş toprakların Xanthium strumarium L. bitkisi kullanılarak fitoremediasyonu. Türkiye Tarımsal Araştırmalar Dergisi, 5(2), 152–157.
  • Hoagland, D.R., & Arnon, D.I. (1938). The water culture method for growing plants without soil. California Agricultural Experiment Station Circular, 347, 1–32.
  • Islam, S., Malik, A.I., Islam, A.K.M.R., & Colmer, T.D. (2007). Salt tolerance in a Hordeum marinum–Triticum aestivum amphiploid and its parents. Journal of Experimental Botany, 58, 1219–1229.
  • Kaçar, B., & Katkat, V. (2023). Bitki besleme (5. baskı). Nobel Akademik Yayıncılık, Ankara, 678 pp.
  • Kumar, S.S., Kadier, A., Malyan, S.K., Ahmad, A., & Bishnoi, N.R. (2017). Phytoremediation and rhizoremediation: Uptake, mobilization and sequestration of heavy metals by plants. In Singh, D., Singh, H., Prabha, R. (eds): Plant–microbe interactions in agro-ecological perspectives. Springer, Singapore, 367–394.
  • Kumar, S.A., Kumar, A., Bhargava, C.R., Patel, R., Dwarka, D., Singh, O., Sharma, S., Jain, S.K., Jain, D., & Ahirwar, M.K. (2023). The effect of various doses of zinc on the growth and yield of soybean crop (Glycine max L.). International Journal of Plant & Soil Science, 35(24), 308–312.
  • Larcher, W. (2003). Physiological plant ecology. Berlin, Springer-Verlag. 107pp.
  • Luna, C., Seffino, L.G., Arias, C., & Taleisnik, E. (2000). Oxidative stress indicators as selection tools for salt tolerance in Chloris gayana. Plant Breeding, 119, 341–345.
  • Lutts, S., Kinet, J.M., & Bouharmont, J. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany, 78, 389–398.
  • Maes, W.H., Achten, W.M.J., Reubens, B., Raes, D., Samson, R., & Muys, B. (2009). Plant–water relationships and growth strategies of Jatropha curcas L. seedlings under different levels of drought stress. Journal of Arid Environments, 73, 877–884.
  • McCauley, A., Jones, C., & Jacobsen, J. (2009). Nutrient management module No. 9. S, 16, Montana State University Extension Service. Publication, Bozeman, USA.
  • Neto, A.D.A., Prisco, J.T., Eneas-Filho, J., Abreu, C.E.B., & Gomez-Filho, E. (2005). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Journal of Experimental Botany, 56, 87–94.
  • Özer, İ. (2025). Effects of foliar application of iron (Fe) and zinc (Zn) on agronomic characteristics of soybean (Glycine max L.). International Journal of Environmental Trends, 9(1), 48–55.
  • Özüstün, A. (2022). Farklı ekolojik koşullarda soya fasulyesi (Glycine max (L.) Merr.) çeşitlerinin verim ve kalite özelliklerinin belirlenmesi (742590). Retrieved from https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
  • Parvaiz, A., & Satyawati, S. (2008). Salt stress and phytochemical responses of plants – a review. Plant, Soil and Environment, 54, 89–99.
  • Ribeiro, R.V., Machado, E.C., Santos, M.G., & Oliveira, R.F. (2009). Photosynthesis and water relations of well-watered orange plants as affected by winter and summer conditions. Photosynthetica, 47, 215–222.
  • Sharma, P., & Dietz, K.-J. (2009). The relationship between metal toxicity and oxidative stress in plants. Journal of Plant Physiology, 166(10), 1132–1141.
  • Sharma, R., Bhardwaj, R., Thukral, A.K., Al-Huqail, A.A., Siddiqui, M.H., & Ahmad, P. (2019). Oxidative stress mitigation and initiation of antioxidant and osmoprotectant responses mediated by ascorbic acid in Brassica juncea L. subjected to copper (II) stress. Ecotoxicology and Environmental Safety, 182, 109436.
  • Taleisnik, E., Peyrano, G., & Arias, C. (1997). Response of Chloris gayana cultivars to salinity. I. Germination and early vegetative growth. Tropical Grasslands, 31, 232–240.
  • Turfan, N. (2022). Bitkilerde ağır metal stresi. In: Seçkin,Dinler, B.&Gül, V. (Ed.) Bitkilerde abiyotik ve biyotik stres yönetimi. Ankara, İKSAD Publishing House, 165–192.
  • Tunçtürk, R., Kulaz, H., & Tunçtürk, M. (2016). Effect of humic acid applications on some nutrient contents of soybean (Glycine max L.) cultivars. Communications, 39(1-II), 503–510.
  • Ünsal, H. (2007). Alkalin topraklarda humik asit ve çinko uygulamalarının iki farklı nohut çeşidinde verim ve N, P, K içeriğine etkisi (213618). Retrieved from https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
  • Vernay, P., Gauthier-Moussard, C., Jean, L., Bordas, F., Faure, O., Ledoigt, G., & Hitmi, A. (2008). Effect of chromium species on phytochemical and physiological parameters in Datura innoxia. Chemosphere, 72(5), 763–771.
  • Verbruggen, N., & Hermans, C. (2008). Proline accumulation in plants: A review. Amino Acids, 35, 753–759.
  • Walia, H., Wilson, C., Condamine, P., Liu, X., Abdelbagi, M.I., Zeng, L., Mandal, J., Xu, J., Cui, X., & Timothy, C.J. (2005). Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage. Plant Physiology, 139, 833–835.
  • Wolterbeek, H.T., & De Bruin, M. (1986). Xylem and phloem import of Na+, K+, Rb+, Cs+ and Sb(SO4)2 in tomato fruits: Differential contributions from stem and leaf. Journal of Experimental Botany, 37, 928–939.
  • Yiğit, A., Erekul, O., & Yaraşır, N. (2021). Kısıtlı Sulama ve Kükürt Dozu Uygulamalarının Soya Fasulyesinde Bitki Gelişimi ve Klorofil (SPAD) İçeriğine Etkisi. ÇOMÜ Ziraat Fakültesi Dergisi, 9(1), 105-117.
Toplam 41 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Bitki Fizyolojisi
Bölüm Araştırma Makalesi
Yazarlar

Mahmut Doğan 0000-0002-4377-2751

Asra Aygün 0009-0000-2192-5297

Gönderilme Tarihi 13 Ekim 2025
Kabul Tarihi 12 Kasım 2025
Erken Görünüm Tarihi 20 Kasım 2025
Yayımlanma Tarihi 18 Aralık 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 9 Sayı: 2

Kaynak Göster

APA Doğan, M., & Aygün, A. (2025). Demir ve Çinko Uygulamalarının Soya [Glycine max (L.) Merr.] Bitkisinin Büyüme ve Gelişimine Etkisi. Commagene Journal of Biology, 9(2), 220-226. https://doi.org/10.31594/commagene.1802380
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