Determination of Extracellular Hydrolytic Enzyme Production Capabilities of Acidithiobacillus Bacteria Isolated from Awasppi River in Sulaymaniyah, Iraq and 16S rDNA Analysis

Öz

In this study, the 16S rDNA gene regions’ phylogenetic positions based on the sequence analysis were determined by examining some hydrolytic extracellular enzyme activities of the Acidithiobacillus bacteria isolated from the water and mud samples obtained from the Awasspi River in Sulaymaniyah province, northern Iraq and from the Darzilais sulphur cave in Sangaw district. While the catalase activity of the isolates was positive; lipase, protease, xylanase, and amylase activities were found to be negative. Genomic DNAs of the isolates were isolated and 16S rDNA gene region was realized with PCR amplification. To determine the distance between the species, Maximum Likelihood algorithm was used to form a phylogenetic tree. It was observed that gene strands belonging to Acidithiobacillus species acquired from NCBI and 2 isolates we used in our study clustered with a strong homology and their genetic positions were revealed. 

Anahtar Kelimeler

• Acidithiobacillus,Sulaimani,Enzyme,16S rDNA

Awa Sppi Nehri’nden (Süleymaniye-Irak) İzole Edilen Acidithiobacillus Cinsi Bakterilerin Ektraselüler Hidrolitik Enzim Üretme Kabiliyetlerinin Belirlenmesi ve 16S rDNA Analizi

Öz

Bu araştırmada, Kuzey Irak'ın Süleymaniye şehrinde bulunan Awasppi nehrinden ve Sangaw'daki Darzilais sülfür kaynaklı mağaradan alınan su ve çamur örneklerinden izole edilen Acidithiobacillus bakterilerinin bazı hidrolitik ektraselüler enzim aktiviteleri incelenerek, 16S rDNA gen bölgelerinin sekans analizi temeline dayalı filogenetik pozisyonları belirlenmiştir. İzolatların katalaz aktivitesi pozitif iken, lipaz, proteaz, ksilanaz ve amilaz aktivitesi negatif olarak belirlenmiştir. İzolatların Genomik DNA’ları izole edilerek 16S rDNA gen bölgesi PZR amplifikasyonu ile gerçekleştirilmiştir. Türler arasındaki uzaklığı belirlemek için Maximum Likelihood algoritması kullanılarak oluşturulan filogenetik ağaç NCBI’dan elde edilmiş Acidithiobacillus türlerine ait gen dizileri ve çalışmamızda kullandığımız 2 izolat güçlü bir homoloji ile kümelendiği gözlemlenerek genetik pozisyonları ortaya konmuştur. Karşılaştırmalı 16S rDNA gen dizisi analizi sonucunda, G1 izolatının Acidithiobacillus thiooxidans’a % 99 benzerlik gösterdiği ve G2 izolatının Acidithiobacillus ferrooxidans’a % 99 benzerlik gösterdiği belirlenmiştir

Anahtar Kelimeler

• Acidithiobacillus,Süleymaniye,Enzim,16S rDNA

Kaynakça

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