Cytotoxic Activities of the Crude Venoms of Macrovipera lebetina lebetina from Cyprus and M. l. obtusa from Turkey (Serpentes: Viperidae) on Human Umbilical Vein Endothelial Cells

Yıl 2019, , 110 - 113, 20.12.2019

Naşit İğci , F. Duygu Özel , Mehmet Zülfü Yıldız

https://doi.org/10.31594/commagene.655929

Cited By: 4

Öz

In this study, we used human umbilical vein endothelial cells (HUVEC) as an in vitro model to compare the cytotoxic activities of the venoms of two Macrovipera lebetina subspecies, M. l. obtusa from southern Anatolia and M. l. lebetina from northern Cyprus. Well-established 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was preferred to assess the cytotoxicity. Our results showed that venom reduced cell viability both in a time and dose-dependent manner. The cytotoxic effect of M. l. lebetina venom on HUVEC is reported for the first time in the present study.

Anahtar Kelimeler

hemorrhage, HUVEC, levantine viper, toxinology

Teşekkür

We greatly acknowledge and dedicate this article to late Prof. Dr. Bayram GÖÇMEN who passed away tragically on 22 March 2019, for his great contributions to the field herpetology and his invaluable support to the studies related to snake venoms in Turkey by providing venom samples. We also thank Ankara University Biotechnology Institute for providing laboratory access and to Dr. Erkan YILMAZ, Ankara University Biotechnology Institute for providing Human umbilical vein endothelial cells.

Kıbrıs’tan Macrovipera lebetina lebetina ve Türkiye’den M. l. obtusa (Serpentes: Viperidae) Ham Zehirlerinin İnsan Kordon Veni Endotel Hücreleri Üzerindeki Sitotoksik Etkileri

Öz

Bu çalışmada, Kuzey Kıbrısʼtan Macrovipera lebetina lebetina ve güneydoğu Anadoluʼdan M. l. obtusa olmak üzere iki M. lebetina alt türünün zehirlerinin (venomlarının) sitotoksik aktivitelerini karşılaştırmak için in vitro bir model olarak insan kordon veni endotel hücreleri (HUVEC) kullandık. Sitotoksik etkinin değerlendirilmesinde iyi bilinen bir yöntem olan 3-(4,5-dimethyl-2- hiazolyl)-2,5-diphenyl-2H-tetrazolium bromür (MTT) testi tercih edilmiştir. Çalışmamızın sonuçları her iki zehrin de hücre canlılığını zamana ve doza bağlı olarak azalttığını göstermiştir. M. l. lebetina zehrinin HUVEC üzerindeki sitotoksik etkisi ilk kez bu çalışmada rapor edilmiştir.

Anahtar Kelimeler

hemoraji, HUVEC, koca engerek, toksinoloji

Kaynakça

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