Kıbrıs’tan Macrovipera lebetina lebetina ve Türkiye’den M. l. obtusa (Serpentes: Viperidae) Ham Zehirlerinin İnsan Kordon Veni Endotel Hücreleri Üzerindeki Sitotoksik Etkileri

Öz

Bu çalışmada, Kuzey Kıbrısʼtan Macrovipera lebetina lebetina ve güneydoğu Anadoluʼdan M. l. obtusa olmak üzere iki M. lebetina alt türünün zehirlerinin (venomlarının) sitotoksik aktivitelerini karşılaştırmak için in vitro bir model olarak insan kordon veni endotel hücreleri (HUVEC) kullandık. Sitotoksik etkinin değerlendirilmesinde iyi bilinen bir yöntem olan 3-(4,5-dimethyl-2- hiazolyl)-2,5-diphenyl-2H-tetrazolium bromür (MTT) testi tercih edilmiştir. Çalışmamızın sonuçları her iki zehrin de hücre canlılığını zamana ve doza bağlı olarak azalttığını göstermiştir. M. l. lebetina zehrinin HUVEC üzerindeki sitotoksik etkisi ilk kez bu çalışmada rapor edilmiştir.

Anahtar Kelimeler

• hemoraji,HUVEC,koca engerek,toksinoloji

Cytotoxic Activities of the Crude Venoms of Macrovipera lebetina lebetina from Cyprus and M. l. obtusa from Turkey (Serpentes: Viperidae) on Human Umbilical Vein Endothelial Cells

Öz

In this study, we used human umbilical vein endothelial cells (HUVEC) as an in vitro model to compare the cytotoxic activities of the venoms of two Macrovipera lebetina subspecies, M. l. obtusa from southern Anatolia and M. l. lebetina from northern Cyprus. Well-established 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was preferred to assess the cytotoxicity. Our results showed that venom reduced cell viability both in a time and dose-dependent manner. The cytotoxic effect of M. l. lebetina venom on HUVEC is reported for the first time in the present study.

Anahtar Kelimeler

• hemorrhage,HUVEC,levantine viper,toxinology

Kaynakça

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