Effects of Acute Sodium Nitrite and Sodium Nitrate on the Development of Drosophila melanogaster

Yıl 2020, Cilt: 4 Sayı: 2, 96 - 99, 31.12.2020

Emel Atlı , Hadi Eshraghi , Alper Orhan

https://doi.org/10.31594/commagene.754697

https://izlik.org/JA69SB53WG

Öz

Sodium nitrite (NaNO2) and sodium nitrate (NaNO3) are used as food preservatives. In addition to teratogenic, mutagenic, and carcinogenic effects, these products are also thought to have endocrine disrupting effects. This study aimed to investigate the effects of sodium nitrite (NaNO2) and sodium nitrate (NaNO3) on the development of Drosophila melanogaster. Third instar larvae of Canton S strain (wild type) were treated to 25 mM, 50 mM, 75 mM NaNO2, and NaNO3 for 6 hours. The pupation and maturation percentages and times were determined. There was no significant difference in the percentage of pupation and maturation compared to the control group (p>0.05). However, 50 and 75 mM NaNO2 exposures caused developmental delay in both the mean pupation and the mean maturation times (p<0.05). Additionally, it was found that mean maturation time was delayed with 50 and 75 mM NaNO3 exposures (p<0.05).

Anahtar Kelimeler

pupation , maturation , developmental time delay , Food additives , Drosophila

Akut Sodyum Nitrat ve Sodyum Nitrit Uygulamasının Drosophila melanogaster’in Gelişimi Üzerine Etkileri

https://izlik.org/JA69SB53WG

Öz

Sodyum nitrit (NaNO2) ve sodyum nitrat (NaNO3) gıda koruyucusu olarak kullanılmaktadır. Teratojenik, mutagenic ve kanserojen etkilere ek olarak, bu ürünlerin endokrin bozucu etkileri de vardır. Bu çalışmada, sodyum nitrit (NaNO2) ve sodyum nitratın (NaNO3) Drosophila melanogaster gelişimi üzerindeki etkileri araştırılmıştır. Yabanıl tip Canton S soyunun üçüncü evre larvalarına, 6 saat boyunca 25 mM, 50 mM, 75 mM NaNO2 ve NaNO3 uygulanmıştır. Pupalaşma ve erginleşme yüzdeleri ve süreleri belirlenmiştir. Pupalaşma ve erginleşme yüzdelerinde kontrol grubuna göre istatistiksel olarak anlamlı bir fark saptanmamıştır (p>0.05). Ancak, 50 ve 75 mM NaNO2 uygulamaları hem ortalama pupalaşma hem de ortalama erginleşme sürelerinde gelişimsel gecikmeye neden olmuştur (p<0.05). Ayrıca ortalama erginleşme süresinin 50 ve 75 mM NaNO3 uygulaması ile ertelendiği saptanmıştır (p<0.05).

Anahtar Kelimeler

Gıda katkı maddeleri , Drosophila , pupalaşma , erginleşme , gelişim zamanı gecikmesi

Kaynakça

• Ansari, F.A., Ali, S.N., & Mahmood, R. (2015). Sodium nitrite induced oxidative stress causes membrane damage, protein oxidation, lipid peroxidation and alters major metabolic pathways in human erythrocytes. Toxicology in Vitro, 29, 1878-1886. https://doi.org/10.1016/j.tiv.2015.07.022
• Atlı, E., & Ünlü, H. (2012). Developmental and Reproductive Effects of Bisphenol A (Bpa) in Drosophila melanogaster. Hacettepe Journal of Biology and Chemistry, 40(1), 61-68.
• Balangoda, A., Deepananda, K.H.M.A., & Wegiriya, H.C.E. (2018). Effects of environmental contamination and acute toxicity of n-nitrate on early life stages of endemic arboreal frog, Polypedatesm cruciger (Blyth, 1852). Bulletin of Environmental Contamination and Toxicology, 100, 195–201. https://doi.org/10.1007/s00128-017-2261-9
• Basak, A.K., Chatterjee, T., Ghosh, S.K., & Chakravarty, A. (2017). Impacts of dietary exposure to sodium or potassium salts of nitrate and nitrite on the development of Drosophila melanogaster. Interdisciplinary Toxicology, 10(2), 70-78. https://doi.org/10.1515/intox-2017-0012
• Brender, J.D., Werler, M.M., Kelley, K.E., Vuong, A.M., Shinde, M.U., Zheng, Q., … Canfield, M.A. (2011). Nitrosatable drug exposure during early pregnancy and neural tube defects in offspring: National Birth Defects Prevention Study. American Journal of Epidemiology, 174(11), 1286–1295.
• Cheng, S.Y., & Chen, J.C. (2002). Accumulations of nitrite and nitrate in the tissues of Penaeus monodon exposed to a combined environment of elevated nitrite and nitrate. Archives of Environmental Contamination and Toxicology, 43, 64–74. https://doi.org/10.1007/s00244-001-0056-8
• Griffis-Kyle, K.L. (2007). Sublethal effects of nitrite on eastern tiger salamander (Ambystoma tigrinum tigrinum) and wood frog (Rana sylvatica) embryos and larvae: implications for field populations. Aquatic Ecology, 41, 119-127. https://doi.org/10.1007/s10452-006-9047-1
• Guillette, L.J., & Edwards, T.M. (2005). Is nitrate an ecologically relevant endocrine disruptor in vertebrates? Integrative and Comparative Biology, 45, 19-27. https://doi.org/10.1093/icb/45.1.19
• Hannas, B.R., Das, P.C., Li, H., & LeBlanc, G.A. (2010). Intracellular conversion of environmental nitrate and nitrite to nitric oxide with resulting developmental toxicity to the crustacean Daphnia magna. Plos One, 5(8), 1-8. https://doi.org/10.1371/journal.pone.0012453
• Helal, E., Zahkok, S., Soliman, G.Z.A., Al-Kassas, M., & Wahed, H. A. (2008). Biochemical studies on the effect of sodium nitrite and/or glutathione treatment on male rats. The Egyptian Journal of Hospital Medicine, 30, 25-38.
• Ikemoto, Y., Teraguchi, M., & Kobayashi, Y. (2002). Plasma levels of nitrate in congenital heart disease: Comparison with healthy children. Pediatric Cardiology, 23(2), 132-136. https://doi.org/10.1007/s00246-001-0036-9
• Özcan, P.Ö. (2011). Propolisin antimutajenik etkilerinin Drosophila melanogaster'de araştırılması (MSc Thesis), Hacettepe University, Ankara, Turkey.
• Özdestan, Ö., & Üren, A. (2010). Nitrate and nitrite in foods. Academic Food Journal, 8(6), 35-43.
• Panesar, N.S., & Chan, K.W. (2000). Decreased steroid hormone synthesis from inorganic nitrite and nitrate: Studies in vitro and in vivo. Toxicology and Applied Pharmacology, 169, 222-230. https://doi.org/10.1006/taap.2000.9079
• Sarıkaya, R., & Cakır, S. (2005). Genotoxicity testing of four food preservatives and their combinations in the Drosophila wing spot test. Environmental Toxicology and Pharmacology, 20, 424-430. https://doi.org/10.1016/j.etap.2005.05.002
• Sarıkaya, R., Cakır, S., & Solak, K. (2006). Gıdalardaki koruyucu maddelerin Drosophila melanogaster’de (mwhxflr) ömür uzunluğuna etkisi. Kastamonu Eğitim Dergisi, 14(1), 173-184.
• Siikavuopio, S.I., & Sæther, B. (2006). Effects of chronic nitrite exposure on growth in juvenile Atlantic cod, Gadus morhua. Aquaculture, 255, 351–356. https://doi.org/10.1016/j.aquaculture.2005.11.058
• Simmons, A.E., Karimi, I., Talwar, M., & Simmons, T.W. (2012). Effects of nitrite on development of embryos and early larval stages of the zebrafish (Danio rerio). Zebrafish, 9, 200–206. https://doi.org/10.1089/zeb.2012.0746
• Suzuki, H., Iijima, K., Scobie, G., & McColl, K.E.L. (2005). Nitrate and nitrosative chemistry within Barrett’s oesophagus during acid reflux. Gut, 54, 1527-1535. https://doi.org/10.1136/gut.2005.066043
• Turp, Y.G., & Sucu, C. (2016). Et Ürünlerinde Nitrat ve Nitrit Kullanımına Potansiyel Alternatif Yöntemler. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 12(2), 231-242.