Metastatik Kastrasyona Dirençli Prostat Kanserinde Narenciye Nobiletin'in Oksidatif Stres Düzeyleri ve Süperoksit Dismutaz Aktiviteleri Üzerindeki Rolü

Öz

Nobiletin (NOB) polimetoksile bir flavondur ve birçok kanser türünde oksidatif stresi modüle edebilen çok sayıda biyolojik aktiviteye sahiptir. Bununla birlikte, literatürde, MCRPC'de Metastatik Kastrasyona Dirençli Prostat Kanserinde (MCRPC) NOB'nin oksidatif stres seviyeleri üzerindeki etkilerine dair henüz bir çalışma bulunmamaktadır. Bu nedenle, ön çalışma olarak MCRPC'de NOB'nin oksidatif stres ve süperoksit dismutaz (SOD) enzim aktiviteleri üzerindeki etkilerini belirlemeyi amaçladık. Bu amaçla, çalışmada NOB'nin Malondialdehit (MDA), hidrojen peroksit (H2O2) ve prolin miktarı ile SOD enzim aktiviteleri üzerindeki etkilerini belirlemek için PC-3 ve HUVEC hücreleri kullanıldı. NOB’un potansiyel olarak SOD enzim aktivitelerini indüklediği, ancak PC-3 hücrelerinde NOB ile inkübasyondan sonra MDA, H2O2 ve prolin seviyesinin azaldığı tespit edildi (p<.05 ve p <.001 istatistiksel olarak anlamlı kabul edildi). Elde edilen veriler, NOB'nin kanser hücreleri için koruyucu bir ajan olarak hareket ettiğini ve MCRPC hücrelerinde oksidan durumunu seçici olarak düzenleyebildiğini doğruladı. Sonuç olarak, bu ön bulgular, MCRPC'de turunçgil NOB'nin oksidatif stres seviyeleri ve antioksidan enzim aktiviteleri üzerindeki rolü hakkında daha iyi fikir vermektedir. Ek olarak, potansiyel bir kemoterapötik ajan olarak NOB'nin bu sitoprotektif etkisinin moleküler mekanizmalarının aydınlatılmasına ihtiyaç vardır.

Anahtar Kelimeler

• Antioksidan Etki;
• Flavanoid;
• Kanser;
• Malondialdehyde;
• Proline

The Role of Citrus Nobiletin on Oxidative Stress Levels and Superoxide Dismutase Activities in Metastatic Castration-Resistant Prostate Cancer

Öz

Nobiletin (NOB) is a polymethoxylated flavone. It has multiple biologic activities that can modulate oxidative stress in many cancer types. However, there is no study in the literature that has examined the effects of NOB on oxidative stress levels in Metastatic Castration-Resistant Prostate Cancer (MCRPC) yet. Motivated from this gap, we investigated the impact of NOB on oxidative stress and superoxide dismutase (SOD) enzyme activities in MCRPC as a preliminary study. For this purpose, PC-3 and HUVEC cells were used to determine the effects of NOB on the amount of Malondialdehyde (MDA), hydrogen peroxide (H2O2), and proline as well as SOD enzyme activities. NOB potentially induced SOD enzyme activities but the level of MDA, H2O2, and proline decreased after incubation with NOB in PC-3 cells (p<.05 and p<.001 were considered statistically significant). Our results confirmed that NOB acted as a protective agent for cancer cells and could selectively regulate oxidant status in MCRPC cells. Consequently, these preliminary findings provide better insight into the role of citrus NOB on oxidative stress levels and antioxidant enzyme activities in MCRPC. Additionally, there is a need to elucidate the molecular mechanisms of this cytoprotective effect of NOB as a potential chemotherapeutic agent.

Anahtar Kelimeler

• Antioxidant Effect;
• Malondialdehyde;
• Cancer;
• Flavanoid;
• Malondialdehyde;
• Proline

Kaynakça

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