Anti-Colorectal Cancer Effects of Medicinal Plants: Euphorbia helioscopia, Ferula elaeochytris, and Sideritis albiflora

Öz

Phytochemicals, extracts, and mixtures obtained from plants have been offered as an option for cancer treatment and prevention for modern drug discovery in recent years. For this purpose, in this study, anti-colorectal cancer effects of the hexane, acetone, methanol, and water extracts obtained by sequential extraction from Euphorbia helioscopia L., Ferula elaeochytris Korovin, and Sideritis albiflora Hub.-Mor. on DLD-1 cell line were investigated in vitro by using Alamar blue assay. Dose-dependent inhibition was detected in the viability of DLD-1 cell line. In all three plants species, E. helioscopia (IC50: 140.83±0.31 µg/mL), F. elaeochytris (IC50: 67.93±0.12 µg/mL), and S. albiflora (IC50: 85.12±0.10 µg/mL) methanol extracts showed higher anti-colorectal effects on DLD-1 cell line compared to other extracts tested for the same species. In addition, the IC50 value of doxorubicin used as a standard was found as 6.10±0.55 µg/mL. With the results obtained, as the first report highlighting in vitro anti-colorectal cancer effects of the studied plant species on DLD-1 cell line, promising marks were obtained from the analysis of the extracts as anti-cancer sources for plant-derived drug applications.

Anahtar Kelimeler

• Plant species
• extracts
• cytotoxic activity
• DLD-1 cell line
• Alamar blue assay

Tıbbi Bitkilerin Anti-Kolorektal Kanser Etkileri: Euphorbia helioscopia, Ferula elaeochytris ve Sideritis albiflora

Öz

Bitkilerden elde edilen fitokimyasallar, ekstreler ve karışımlar, son yıllarda modern ilaç keşfi için kanser tedavisinde ve önlenmesinde bir seçenek olarak sunulmuştur. Bu amaçla, bu çalışmada sıralı ekstraksiyon kullanılarak Euphorbia helioscopia L., Ferula elaeochytris Korovin ve Sideritis albiflora Hub.-Mor. bitkilerinden elde edilen hekzan, aseton ve metanol ekstrelerinin DLD-1 hücre hattı üzerindeki anti-kolorektal kanser etkileri Alamar mavisi testi kullanılarak in vitro olarak incelendi. DLD-1 hücre hattının canlılığında doza bağlı inhibisyon tespit edildi. Üç bitki türünün hepsinde, E. helioscopia (IC50: 140.83±0.31 µg/mL), F. elaeochytris (IC50: 67.93±0.12 µg/mL) ve S. albiflora (IC50: 85.12±0.10 µg/mL) metanol ekstreleri aynı türler için test edilen diğer ekstrelere kıyasla DLD-1 hücre hattı üzerine daha yüksek anti-kolorektal kanser etkisi gösterdi. İlaveten, standart olarak kullanılan doksorubisinin IC50 değeri 6.10±0.55 µg/mL olarak belirlendi. İncelenen bitki türlerinin DLD-1 hücre hattı üzerindeki in vitro anti-kolorektal kanser etkilerini vurgulayan bu ilk çalışma ile bitki kaynaklı ilaç uygulamaları için anti-kanser kaynakları olarak ekstraktların analizinden umut verici sonuçlar ortaya çıkarıldı.

Anahtar Kelimeler

• Bitki türleri
• ekstre
• sitotoksik aktivite
• DLD-1 hücre hattı
• Alamar mavisi testi

Kaynakça

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