Determination of Pesticide-Induced Genotoxicity on Soybean (Glycine max L.)

Yıl 2019, Cilt: 3 Sayı: 2, 83 - 87, 20.12.2019

Asuman Deveci Özkan Özlem Aksoy

https://doi.org/10.31594/commagene.615488

Cited By: 1

Öz

Pesticides are used in agriculture and cause
side effects in plants and can be transported to products which we consume. Genotoxic
chemical substances distributed to environment and higher plants such as Glycine max have been used as an
indicator plants that show the genotoxic effects of environmental chemical
pollutants. In this respect we investigated the potential genotoxic effect of
three different pesticides (Pomarsol Forte WP 80 as a fungucide, Arrivo 25 EC
as an insecticide, and The End EC as an herbicide) on G. max (Glycine max L.) for the first time. In order to
determine the genotoxic effects of these pesticides on G max. Median EC
(effective concentration) determination analysis, RAPD-PCR (randomly amplified
polymorphic DNA-polymerase chain reaction) assay and protein analysis were
used. Our results indicated that The End as a herbicide had more inhibitory
effects on G. max root growth compare
to the other pesticides. 20 RAPD primers were used, eighteen primers gave
stable results while 11 of them were polymorphic and 7 of them showed the same
band profile. Percentage of polymorphism was found as 20%. Total protein
content was significantly decreased by insecticide treatment but increased in
herbicide treatment (p<0.05). In
conclusion these results suggest that these pesticides have genotoxic effects
on G. max and the use of these
chemicals must be reduced to avoid exposure to humans and the environment.

Anahtar Kelimeler

cypermethrin, quizalofop-p-ethyl, RAPD-PCR, SDS-PAGE, thiram

Destekleyen Kurum

Kocaeli University Scientific Research Projects Coordination Unit

Proje Numarası

2011/43

Teşekkür

This study is supported by Kocaeli University Scientific Research Projects Coordination Unit. Project Number: 2011/43.

Pestisit-Kaynaklı Genotoksisitenin Soya Fasulyesinde (Glycine max L.) Belirlenmesi

Öz

Pestisitler, tarımsal alanlarda yaygın olarak
kullanılırken bitkilerde yan etkilere neden olmaktadır ve tükettiğimiz ürünlere
de taşınabilmektedir. Genotoksik kimyasal maddeler çevreye yayılmaktadır ve Glycine max gibi yüksek yapılı bitkiler,
kimyasal çevre kirleticilerinin genotoksik etkilerini gösteren indikatör
bitkiler olarak kullanılmaktadır. Bu bağlamda, mevcut çalışmada ilk kez üç
farklı pestisitin (fungusit olarak Pomarsol Forte WP 80, insektisit olarak
Arrivo 25 EC ve herbisit olarak The End EC) potansiyel genotoksik etkisi soya
fasulyesi (Glycine max L.) üzerinde
araştırılmıştır. G. max üzerindeki genotoksik etkiyi belirlemek için
ortalama etkili konsantrasyon (median EC), RAPD-PCR (rastgele amplifiye
polimorfik DNA-polimeraz zincir reaksiyonu) ve protein analizleri
kullanılmıştır. Elde edilen sonuçlar bir herbisit olarak The END pestisitinin
diğerlerine göre G. max kök büyümesi
üzerinde daha fazla inhibe edici etkisi olduğu saptanmıştır. 20 RAPD primeri
kullanılmış; bunlardan 18 primer stabil sonuç verirken, 11 tanesi polimorfik ve
7 tanesi benzer bant profili göstermiştir. Polimorfizm yüzdesi, % 20 olarak
bulunmuştur. Toplam protein içeriği insektisit muamelesi ile kontrol grubuna
göre belirgin şekilde azalırken, herbisit muamelesi sonrası anlamlı olarak
artmıştır (p <0.05). Sonuç olarak elde edilen veriler bu pestisitlerin G. max üzerinde belirgin genotoksik
etkilerinin olduğunu ve bu kimyasallara karşı insan ve çevresel maruziyetin
atmasından dolayı kullanımdan kaçınılması gerektiğini göstermektedir.

Anahtar Kelimeler

cypermethrin, quizalofop-p-ethyl, RAPD-PCR, SDS-PAGE, thiram

Proje Numarası

2011/43

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