Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora)
Besiyeri Bileşiminin Çayır Salkımotunda (Poa pratensis L. cv. Evora) in vitro Tohum Çimlenmesi ve Bitki Gelişimi Üzerindeki Etkisi

Arda ACEMİ [1]


In the current study, the effects of De Greef & Jacobs (DG), Linsmaier & Skoog (LS), Murashige & Skoog (MS), and Schenk & Hildebrandt (SH) media were tested on seed germination and plant development in Poa pratensis cv. Evora. The highest germination rate (83±2.74%) was found on SH medium, whereas LS medium gave the lowest (46±4.18%) germination rate. The statistically same leaf numbers were recorded from SH (2.10±0.27) and DG (2.12±0.18) media. SH and DG media gave 4.28±0.28 cm and 4.16±0.31 cm mean leaf lengths, respectively. SH medium gave the maximum mean root number (3.09±0.26). However, the LS medium gave the lowest mean root number (1.84±0.10). The longest roots (1.43±0.19 cm) were observed in the plants grown in SH medium. However, DG medium had the minimum mean root length (0.81±0.08 cm). In conclusion, SH medium should be preferred over the other medium tested in in vitro tissue culture studies on the species to increase the biomass production yield. The efficacy of SH medium in the in vitro propagation of P. pratensis could be increased using growth promoters.
Bu çalışmada, Poa pratensis cv. Evora’da De Greef & Jacobs (DG), Linsmaier & Skoog (LS), Murashige & Skoog (MS) ve Schenk & Hildebrandt (SH) besiyerlerinin tohum çimlenmesi ve bitki gelişimi üzerindeki etkileri test edilmiştir. En yüksek çimlenme oranı (%83±2.74) SH ortamında bulunurken, LS ortamı en düşük (%46±4.18) çimlenme oranını vermiştir. Ortalama yaprak sayıları SH (2.10±0.27) ve DG (2.12±0.18) ortamlarından istatistiksel olarak aynı kaydedilmiştir. Ortalama yaprak uzunlukları SH ve DG ortamlarında sırasıyla 4.28±0.28 cm ve 4.16±0.31 cm olarak ölçülmüştür. Maksimum ortalama kök sayısı (3.09±0.26) SH ortamından elde edilmiştir. Bununla birlikte, en düşük ortalama kök sayısı (1.84±0.10) LS ortamında kaydedilmiştir. En uzun kökler (1.43±0.19 cm) SH ortamında yetiştirilen bitkilerde gözlenmiştir. Bununla birlikte, DG ortamı minimum ortalama kök uzunluğunu (0.81±0.08 cm) vermiştir. Sonuç olarak, biyokütle üretimini artırmak için bu tür üzerinde yapılan in vitro doku kültürü çalışmalarında test edilen diğer besiyerlerine kıyasla SH besiyeri tercih edilmelidir. SH ortamının P. pratensis'in in vitro gelişimindeki etkinliği büyüme destekleyicileri kullanılarak artırılabilir.
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Birincil Dil en
Konular Biyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Orcid: 0000-0003-0270-8507
Yazar: Arda ACEMİ (Sorumlu Yazar)
Kurum: KOCAELİ ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Ülke: Turkey


Teşekkür The author would like to thank Duhan Tırlı and Serdar Yıldız for their help with the inoculation process of the cultures used in this paper.
Tarihler

Başvuru Tarihi : 18 Kasım 2020
Kabul Tarihi : 12 Ocak 2021
Yayımlanma Tarihi : 31 Mart 2021

Bibtex @araştırma makalesi { commagene827909, journal = {Commagene Journal of Biology}, issn = {}, eissn = {2602-456X}, address = {Adıyaman Bilimsel Araştırmalar Derneği,}, publisher = {ABADER (Adıyaman Bilimsel Araştırmalar Derneği)}, year = {2021}, volume = {Erken Görünüm}, pages = {1 - 6}, doi = {10.31594/commagene.827909}, title = {Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora)}, key = {cite}, author = {Acemi, Arda} }
APA Acemi, A . (2021). Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora) . Commagene Journal of Biology , Erken Görünüm , 1-6 . DOI: 10.31594/commagene.827909
MLA Acemi, A . "Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora)" . Commagene Journal of Biology Erken Görünüm (2021 ): 1-6 <https://dergipark.org.tr/tr/pub/commagene/issue/60100/827909>
Chicago Acemi, A . "Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora)". Commagene Journal of Biology Erken Görünüm (2021 ): 1-6
RIS TY - JOUR T1 - Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora) AU - Arda Acemi Y1 - 2021 PY - 2021 N1 - doi: 10.31594/commagene.827909 DO - 10.31594/commagene.827909 T2 - Commagene Journal of Biology JF - Journal JO - JOR SP - 1 EP - 6 VL - Erken Görünüm IS - SN - -2602-456X M3 - doi: 10.31594/commagene.827909 UR - https://doi.org/10.31594/commagene.827909 Y2 - 2021 ER -
EndNote %0 Kommagene Biyoloji Dergisi Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora) %A Arda Acemi %T Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora) %D 2021 %J Commagene Journal of Biology %P -2602-456X %V Erken Görünüm %N %R doi: 10.31594/commagene.827909 %U 10.31594/commagene.827909
ISNAD Acemi, Arda . "Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora)". Commagene Journal of Biology Erken Görünüm / (Mart 2021): 1-6 . https://doi.org/10.31594/commagene.827909
AMA Acemi A . Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora). Comm. J. Biol.. 2021; Erken Görünüm: 1-6.
Vancouver Acemi A . Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora). Commagene Journal of Biology. 2021; Erken Görünüm: 1-6.
IEEE A. Acemi , "Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora)", Commagene Journal of Biology, c. Erken Görünüm, ss. 1-6, Mar. 2021, doi:10.31594/commagene.827909